Blog

Published: 2020-04-02

Uppsala University is a comprehensive research-intensive university with a strong international standing. Our mission is to pursue top-quality research and education and to interact constructively with society. Our most important assets are all the individuals whose curiosity and dedication make Uppsala University one of Sweden’s most exciting workplaces. Uppsala University has 46.000 students, 7.300 employees and a turnover of SEK 7.3 billion.

The Department of Immunology, Genetics and Pathology at Uppsala University (www.igp.uu.se) has a broad research profile with strong research groups focused on cancer, autoimmune and genetic diseases. A fundamental idea at the department is to stimulate translational research and thereby closer interactions between medical research and health care. Research is presently conducted in the following areas: medical and clinical genetics, clinical immunology, pathology, neuro-oncology, vascular biology, radiation science and molecular tools. Department activities are also integrated with the units for Oncology, Clinical Genetics, Clinical Immunology, Clinical Pathology, and Hospital Physics at Akademiska sjukhuset, Uppsala. The department has teaching assignments in several education programmes, including Master Programmes, at the Faculty of Medicine, and in a number of educations at the Disciplinary Domain of Science and Technology. The department has a yearly turnover of around SEK 420 million, out of which more than half is made up of external funding. The staff amounts to approximately 345 employees, out of which 100 are PhD-students, and there are in total more than 700 affiliated people.

Project description: Diabetes is the leading cause of kidney disease, and about 1 in 4 adults with diabetes has kidney disease. So far, genome-wide association studies identified at least 16 DNA regions that are robustly associated with the risk of diabetic kidney disease. However, only a few of these DNA regions contain genes known to play a role in kidney disease. Identifying and characterizing mechanisms by which causal genes act is essential, since such genes may encode targets that can be translated into efficient medication in the future, and there are currently no drugs that can prevent or cure diabetic kidney disease. What’s more, model systems to systematically characterise candidate genes in vivo are not currently available.
In this project, we aim to develop and validate an image and CRISPR-Cas9-based zebrafish model system that is suitable for systematic characterization of genes and drugs with an anticipated role in kidney disease, and to use this model system to characterise candidate genes.

Work description: To make this project a success, the candidate will: 1) identify traits of relevance for kidney disease that can be visualised in zebrafish embryos or larvae using an automated microscopy set-up; 2) help develop scripts to automatically quantify such traits objectively and in high throughput, in collaboration with local image-analysis experts; 3) develop additional readouts that can be characterised at the single larval level after imaging; 4) characterise the effect of proof-of-concept exposures (i.e. metabolic challenges, exposure to chemicals or drugs, CRISPR-induced mutations in proof of concept genes) on anticipated disease-related traits; 5) use validated readouts to characterise previously unanticipated candidate genes.
This workflow additionally requires the successful candidate to 6) micro-inject to generate CRISPR founders; 7) prepare samples for downstream biochemical and sequencing analyses using a pipetting robot; 8) manage and statistically analyse large amounts of data efficiently; 9) place new results in the context of the existing knowledge base; and 10) disseminate new insights into manuscripts for publication in peer-reviewed journals and presentations at (inter)national conferences.

Qualifications: Applications are accepted from highly motivated candidates with a PhD in Molecular Biology, Molecular Epidemiology, or similar. The PhD must have been completed within three years of the application deadline. If you received your PhD earlier but special circumstances apply (i.e. prolonged periods of illness, parental leave, military service, union duties and others of similar character) then you may also be eligible to apply. Applicants must have a documented and broad competence in basic molecular biology methodology, including working experience with the zebrafish as a model system, DNA mutagenesis, and fluorescence or confocal microscopy. A successful candidate should be a highly motivated, organised, reliable team player that can also work independently and is proficient in communicating in English, both orally and in writing. Prior knowledge of and experience in working with the kidneys,  data management, microinjections, epidemiology, biostatistics and/or image- based analyses are considered additional strenghts.

The application should include a cover letter describing yourself, your research interests and your experience relevant to this position (as described above); a CV; a list of publications in peer-reviewed journals; and contact details for at least two reference persons. If available, letters of recommendation can also be included.

Salary: Individual salary.

Starting date: 01-06-2020 or as otherwise agreed.

Type of employment: Temporary position of 2 years according to central collective agreement.

Scope of employment: 100 %

For further information about the position please contact: Marcel den Hoed, marcel.den_hoed@igp.uu.se, 070-4250752.

Please submit your application by 23 April 2020, UFV-PA 2020/1041.

Are you considering moving to Sweden to work at Uppsala University? If so, you will find a lot of information about working and living in Sweden at www.uu.se/joinus. You are also welcome to contact International Faculty and Staff Services at ifss@uadm.uu.se.

Please do not send offers of recruitment or advertising services.

Submit your application through Uppsala University´s recruitment system, which can be accessed using the following link:

https://www.uu.se/en/about-uu/join-us/details/?positionId=323985


Placement: Department of Immunology, Genetics and Pathology

Type of employment: Full time , Temporary position longer than 6 months

Pay: Fixed salary

Number of positions: 1

Working hours: 100%

Town: Uppsala

County: Uppsala län

Country: Sweden

Union representative: Seko Universitetsklubben seko@uadm.uu.se
ST/TCO tco@fackorg.uu.se
Saco-rådet saco@uadm.uu.se

Number of reference: UFV-PA 2020/1041

Last application date: 2020-04-23

Published: 2020-04-02

Uppsala University is a comprehensive research-intensive university with a strong international standing. Our mission is to pursue top-quality research and education and to interact constructively with society. Our most important assets are all the individuals whose curiosity and dedication make Uppsala University one of Sweden’s most exciting workplaces. Uppsala University has 46.000 students, 7.300 employees and a turnover of SEK 7.3 billion.

The Department of Immunology, Genetics and Pathology at Uppsala University (www.igp.uu.se) has a broad research profile with strong research groups focused on cancer, autoimmune and genetic diseases. A fundamental idea at the department is to stimulate translational research and thereby closer interactions between medical research and health care. Research is presently conducted in the following areas: medical and clinical genetics, clinical immunology, pathology, neuro-oncology, vascular biology, radiation science and molecular tools. Department activities are also integrated with the units for Oncology, Clinical Genetics, Clinical Immunology, Clinical Pathology, and Hospital Physics at Akademiska sjukhuset, Uppsala. The department has teaching assignments in several education programmes, including Master Programmes, at the Faculty of Medicine, and in a number of educations at the Disciplinary Domain of Science and Technology. The department has a yearly turnover of around SEK 420 million, out of which more than half is made up of external funding. The staff amounts to approximately 345 employees, out of which 100 are PhD-students, and there are in total more than 700 affiliated people.

Project description: Diabetes is the leading cause of kidney disease, and about 1 in 4 adults with diabetes has kidney disease. So far, genome-wide association studies identified at least 16 DNA regions that are robustly associated with the risk of diabetic kidney disease. However, only a few of these DNA regions contain genes known to play a role in kidney disease. Identifying and characterizing mechanisms by which causal genes act is essential, since such genes may encode targets that can be translated into efficient medication in the future, and there are currently no drugs that can prevent or cure diabetic kidney disease. What’s more, model systems to systematically characterise candidate genes in vivo are not currently available.
In this project, we aim to develop and validate an image and CRISPR-Cas9-based zebrafish model system that is suitable for systematic characterization of genes and drugs with an anticipated role in kidney disease, and to use this model system to characterise candidate genes.

Work description: To make this project a success, the candidate will: 1) identify traits of relevance for kidney disease that can be visualised in zebrafish embryos or larvae using an automated microscopy set-up; 2) help develop scripts to automatically quantify such traits objectively and in high throughput, in collaboration with local image-analysis experts; 3) develop additional readouts that can be characterised at the single larval level after imaging; 4) characterise the effect of proof-of-concept exposures (i.e. metabolic challenges, exposure to chemicals or drugs, CRISPR-induced mutations in proof of concept genes) on anticipated disease-related traits; 5) use validated readouts to characterise previously unanticipated candidate genes.
This workflow additionally requires the successful candidate to 6) micro-inject to generate CRISPR founders; 7) prepare samples for downstream biochemical and sequencing analyses using a pipetting robot; 8) manage and statistically analyse large amounts of data efficiently; 9) place new results in the context of the existing knowledge base; and 10) disseminate new insights into manuscripts for publication in peer-reviewed journals and presentations at (inter)national conferences.

Qualifications: Applications are accepted from highly motivated candidates with a PhD in Molecular Biology, Molecular Epidemiology, or similar.
Applicants must have a documented and broad competence in basic molecular biology methodology, including working experience with the zebrafish as a model system, DNA mutagenesis, and fluorescence or confocal microscopy. A successful candidate should be a highly motivated, organised, reliable team player that can also work independently and is proficient in communicating in English, both orally and in writing. Prior knowledge of and experience in working with the kidneys,  data management, microinjections, epidemiology, biostatistics and/or image- based analyses are considered additional strenghts.

The application should include a cover letter describing yourself, your research interests and your experience relevant to this position (as described above); a CV; a list of publications in peer-reviewed journals; and contact details for at least two reference persons. If available, letters of recommendation can also be included.

Salary: Individual salary.

Starting date: 01-06-2020 or as otherwise agreed.

Type of employment: Temporary position ending 31-05-2022.

Scope of employment: 100 %

For further information about the position please contact: Marcel den Hoed, marcel.den_hoed@igp.uu.se, 070-4250752.

Please submit your application by 23 April 2020, UFV-PA 2020/1042.

Are you considering moving to Sweden to work at Uppsala University? If so, you will find a lot of information about working and living in Sweden at www.uu.se/joinus. You are also welcome to contact International Faculty and Staff Services at [ifss@uadm.uu.se|mailto:ifss@uadm.uu.se].

Please do not send offers of recruitment or advertising services.

Submit your application through Uppsala University´s recruitment system, which can be accessed using the link below:

https://www.uu.se/en/about-uu/join-us/details/?positionId=323980


Placement: Department of Immunology, Genetics and Pathology

Type of employment: Full time , Temporary position longer than 6 months

Pay: Individual salary

Number of positions: 1

Working hours: 100%

Town: Uppsala

County: Uppsala län

Country: Sweden

Union representative: Seko Universitetsklubben seko@uadm.uu.se
ST/TCO tco@fackorg.uu.se
Saco-rådet saco@uadm.uu.se

Number of reference: UFV-PA 2020/1042

Last application date: 2020-04-23  Page Title

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A postdoctoral position is available in the group of Professor Felix B. Engel. The team studies heart development and applies this knowledge to enhance endogenous cardiac repair processes or to engineer cardiac tissues for transplantation. Main topics are currently adhesion GPCRs, cardiomyocyte proliferation, centrosome biology/non-centrosomal microtubule-organizing center, electroconductive hydrogels, and spider silk based materials. To achieve this, the group utilizes for example zebrafish and mouse as model organisms, hiPSCs, as well as state-of-the-art molecular and cell biology techniques. The Engel group is located in the Translational Research Center of the University Hospital Erlangen. Our laboratory publishes regularly in high profile journals such as Cell Research, Advanced Functional Materials, PNAS, eLife, Development, and Biomaterials.


Background of the project: Cardiomyocytes establish after birth a cell cycle arrest associated with cytokinesis failure and centrosome disintegration, which appears strict as cardiac tumors are very rare. In addition, it is extremely difficult to induce tumor-relevant processes like proliferation and migration in adult cardiomyocytes. Thus, we hypothesize that cardiomyocyte differentiation represent an excellent model to identify new potential targets to treat cancer.


Funding: The project is funded by the Wilhelm Sander-Foundation. Salary: E13, 100% for 2 years with possible extension.


Job description: We are seeking a highly motivated and ambitious postdoctoral candidate with a strong background in cell, developmental and/or molecular biology with experience in life cell imaging, the model system zebrafish and/or cancer biology to join our team. The candidate will investigate the effect of candidate genes obtained from studying heart development on colorectal cancer growth and metastasis in an embryonic zebrafish xenograft model and in vitro systems.Starting date for this position is the 1 July 2020, or upon agreement with the chosen candidate.


Application: Please contact Prof. Engel for further information and send your application preferable by email to:


Prof. Dr. rer. nat. Dipl. Ing. Felix B. Engel

Universitätsklinikum Erlangen

Experimentelle Nieren- und Kreislaufforschung

Nephropathologische Abteilung, Pathologisches Institut

Schwabachanlage 12 (TRC)

91054 Erlangen


phone:            +49 (0) 9131-8525699  

e-mail:             felix.engel@uk-erlangen.de


Deadline for applications: 9 May 2020, 23.59 pm.

 

 

Research Technician position investigating the mechanisms underlying cardiac development and disease

The Bloomekatz laboratory in the Department of Biology at the University of Mississippi in Oxford, MS is seeking a research technician to assist in our investigations of cardiac morphogenesis and disease using zebrafish. We utilize an innovative combination of genetic, live-imaging, biophysical and system-biology approaches to elucidate the mechanisms underlying morphogenetic and identity transformations in the heart. Please see our website https://thebloomekatzlaboratory.org for details on our research. The successful candidate will have the opportunity to be involved in all aspects of the research and publication process, from experimental design to data analysis and publication. Duties may involve - conducting developmental/cell biological experiments, analyzing imaging and large datasets, zebrafish husbandry.  

-Interested in joining our dynamic group apply online: https://careers.olemiss.edu, search zebrafish.  Please submit a cover letter indicating your scientific interests and how you think they fit with those of the Bloomekatz laboratory and a resume/CV indicating your scientific background. Salary dependent on experience. This position is eligible for benefits. Anticipated start date Summer or Fall 2020. This is a yearly position, which may renew dependent on funding.

-Further questions can be directed to Dr. Bloomekatz at josh@olemiss.edu. The University of Mississippi is an EOE/AA/Minorities/Females/Vet/Disability/Sexual Orientation/Gender Identity/Title VI/Title VII/Title IX/ADA/ADEA employer.

Uppsala University is a comprehensive research-intensive university with a strong international standing. Our mission is to pursue top-quality research and education and to interact constructively with society. Our most important assets are all the individuals whose curiosity and dedication make Uppsala University one of Sweden’s most exciting workplaces. Uppsala University has 46.000 students, 7.300 employees and a turnover of SEK 7.3 billion.

The Department of Immunology, Genetics and Pathology at Uppsala University (www.igp.uu.se) has a broad research profile with strong research groups focused on cancer, autoimmune and genetic diseases. A fundamental idea at the department is to stimulate translational research and thereby closer interactions between medical research and health care. Research is presently conducted in the following areas: medical and clinical genetics, clinical immunology, pathology, neuro-oncology, vascular biology, radiation science and molecular tools. Department activities are also integrated with the units for Oncology, Clinical Genetics, Clinical Immunology, Clinical Pathology, and Hospital Physics at Akademiska sjukhuset, Uppsala. The department has teaching assignments in several education programmes, including Master Programmes, at the Faculty of Medicine, and in a number of educations at the Disciplinary Domain of Science and Technology. The department has a yearly turnover of around SEK 420 million, out of which more than half is made up of external funding. The staff amounts to approximately 345 employees, out of which 100 are PhD-students, and there are in total more than 700 affiliated people.

1-2 Research technician positions in translational genomics - Translation of findings from largescale genetic association studies for cardiometabolic diseases in humans using zebrafish model systems.

Project description: Genome-wide association studies (GWAS) have identified hundreds of genetic loci that are robustly associated with cardiometabolic risk factors and diseases. With few exceptions, the causal genes in these loci are currently unknown. Before results from GWAS can be translated into the clinic - for example as novel biomarkers or drug targets - we need to identify and characterise causal genes and mechanisms. Recent developments in CRISPR-Cas9-based mutagenesis, high-throughput imaging, and image-based analyses have highlighted the zebrafish as a promising model system for systematic, large-scale genetic screens in vivo.

The overall aim of the project is to identify and characterise causal genes in GWAS-identified loci for cardiometabolic risk factors and diseases using zebrafish model systems. 

The planned project aims to use image- and CRISPR-Cas9 based zebrafish model systems we developed and validated in-house to characterise 200 candidate genes with an anticipated role in cardiometabolic diseases. Successful candidates will work together in a team that will additionally consist of a research engineer and the PI.

Duties: Tasks performed by the team include: 1) designing of CRISPR-Cas9 guide RNAs (gRNAs) to target candidate genes; 2) gRNA synthesis and microinjection with Cas9 mRNA; 3) testing mutagenic efficiency of gRNAs using a fragment length PCR analysis; 4) raising CRISPR founders to adulthood, i. e. zebrafish husbandry; 5) crossing founders, pre-screening for the presence of fluorescently labelled transgenes, and preparing larvae for screening; 6) imaging larvae for disease-related traits using an automated positioning system and fluorescence microscope; 7) preparing samples for additional experiments - like biochemistry; and multiplexed, paired-end sequencing – using a robot;

8) objective quantification of imaging data using existing pipelines; 9) data management and quality control. Weekend work can occur.

Successful candidates will be placed in the den Hoed research group (http://igp.uu.se/forskning/ genetik_genomik/marcel-den-hoed/). The project is funded by a collaboration with industry.

Requirements: Applications are accepted from highly motivated candidates with a degree in Biomedicine, (Molecular) Medicine, Molecular (Cell) Biology, Marine Biology or similar and at least two years of relevant work experience. Written and oral proficiency in English is a prerequisite, as are basic knowledge of, and skills in molecular biology or fluorescence microscopy. A successful candidate should be a highly motivated, organised, reliable team player that can also work independently. Prior knowledge of and experience in zebrafish husbandry is required. Prior knowledge of and experience with microinjections, CRISPR-based mutagenesis, data handling, epidemiology, biostatistics and/or image-based analyses.

The application should include a cover letter describing yourself, your research interests, your experience with each of the tasks described above, and your availability. A CV; a verified list of course grades; and contact details for at least two reference persons should also be included. If available, a BSc and/or MSc certificate, and letters of recommendation can also be included.

Salary: Individual salary.

Starting date: 01-05-2020 or as otherwise agreed.

Type of employment: Temporary position ending 48 months.

Scope of employment: 100 %

For further information about the position please contact: Marcel den Hoed, PhD, phone +46-70-425 07 52,  marcel.den_hoed@igp.uu.se

Please submit your application by April 6 2020, UFV-PA 2020/834.

Are you considering moving to Sweden to work at Uppsala University? If so, you will find a lot of information about working and living in Sweden at www.uu.se/joinus. You are also welcome to contact International Faculty and Staff Services at ifss@uadm.uu.se.

Please do not send offers of recruitment or advertising services.

Submit your application through Uppsala University´s recruitment system, which can be accessed here:

https://www.uu.se/en/about-uu/join-us/details/?positionId=321160

 

Placement: Department of Immunology, Genetics and Pathology

Type of employment: Full time , Temporary position longer than 6 months

Pay: Individual salary

Number of positions: 1

Working hours: 100%

Town: Uppsala

County: Uppsala län

Country: Sweden

Union representative: Seko Universitetsklubben seko@uadm.uu.se
ST/TCO tco@fackorg.uu.se
Saco-rådet saco@uadm.uu.se

Number of reference: UFV-PA 2020/834

Last application date: 2020-04-06




Uppsala University is a comprehensive research-intensive university with a strong international standing. Our mission is to pursue top-quality research and education and to interact constructively with society. Our most important assets are all the individuals whose curiosity and dedication make Uppsala University one of Sweden’s most exciting workplaces. Uppsala University has 46.000 students, 7.300 employees and a turnover of SEK 7.3 billion.

The Department of Immunology, Genetics and Pathology at Uppsala University (www.igp.uu.se) has a broad research profile with strong research groups focused on cancer, autoimmune and genetic diseases. A fundamental idea at the department is to stimulate translational research and thereby closer interactions between medical research and health care. Research is presently conducted in the following areas: medical and clinical genetics, clinical immunology, pathology, neuro-oncology, vascular biology, radiation science and molecular tools. Department activities are also integrated with the units for Oncology, Clinical Genetics, Clinical Immunology, Clinical Pathology, and Hospital Physics at Akademiska sjukhuset, Uppsala. The department has teaching assignments in several education programmes, including Master Programmes, at the Faculty of Medicine, and in a number of educations at the Disciplinary Domain of Science and Technology. The department has a yearly turnover of around SEK 420 million, out of which more than half is made up of external funding. The staff amounts to approximately 345 employees, out of which 100 are PhD-students, and there are in total more than 700 affiliated people.

1-3 Research assistant positions in translational genomics - Translation of findings from large-scale genetic association studies for cardiometabolic diseases in humans using zebrafish model systems

Project description: Genome-wide association studies (GWAS) have identified hundreds of genetic loci that are robustly associated with cardiometabolic risk factors and diseases. With few exceptions, the causal genes in these loci are currently unknown. Before results from GWAS can be translated into the clinic - for example as novel biomarkers or drug targets - we need to identify and characterise causal genes and mechanisms. Recent developments in CRISPR-Cas9-based mutagenesis, high-throughput imaging, and image-based analyses have highlighted the zebrafish as a promising model system for systematic, large-scale genetic screens in vivo.

The overall aim of the project is to identify and characterise causal genes in GWAS-identified loci for cardiometabolic risk factors and diseases using zebrafish model systems.

The planned project aims to use image- and CRISPR-Cas9 based zebrafish model systems we developed and validated in-house to characterise 200 candidate genes with an anticipated role in cardiometabolic diseases. Successful candidates will work together in a team that will additionally consist of a research engineer and the PI.

Duties: Tasks performed by the team include: 1) designing of CRISPR-Cas9 guide RNAs (gRNAs) to target candidate genes; 2) gRNA synthesis and microinjection with Cas9 mRNA; 3) testing mutagenic efficiency of gRNAs using a fragment length PCR analysis; 4) raising CRISPR founders to adulthood, i. e. zebrafish husbandry; 5) crossing founders, pre-screening for the presence of fluorescently labelled transgenes, and preparing larvae for screening; 6) imaging larvae for disease-related traits using an automated positioning system and fluorescence microscope; 7) preparing samples for additional experiments - like biochemistry; and multiplexed, paired-end sequencing – using a robot; 8) objective quantification of imaging data using existing pipelines; 9) data management and quality control. Weekend work can occur.

Successful candidates will be placed in the den Hoed research group (https://igp.uu.se/forskning/ genetik_genomik/marcel-den-hoed/). The project is funded by a collaboration with industry.

Requirements: Applications are accepted from highly motivated candidates with a degree in Biomedicine, (Molecular) Medicine, Molecular (Cell) Biology, Marine Biology or similar. Written and oral proficiency in English is a prerequisite, as are basic knowledge of, and skills in molecular biology or fluorescence imaging. A successful candidate should be a highly motivated, organised, reliable team player that can also work independently. Prior knowledge and experience in CRISPR-based mutagenesis, microinjections, data handling, epidemiology, biostatistics, zebrafish husbandry and/or image-based analyses are a bonus.

The application should include a cover letter describing yourself, your research interests, your experience with each of the requirements described above, and your availability. A CV, a verified list of course grades, and contact details for at least two reference persons should also be included. If available, a BSc and/or MSc certificate, and letters of recommendation can also be included.

Salary: Individual salary.

Starting date: 01-05-2020 or as otherwise agreed.

Type of employment: Temporary position ending 48 months.

Extent of employment: 100 %.

For further information about the position please contact: Marcel den Hoed, PhD, phone +46-70-425 07 52, marcel.den_hoed@igp.uu.se.

Please submit your application by April 6 2020, UFV-PA 2020/833.

Are you considering moving to Sweden to work at Uppsala University? If so, you will find a lot of information about working and living in Sweden at www.uu.se/joinus. You are also welcome to contact International Faculty and Staff Services at ifss@uadm.uu.se.

Please do not send offers of recruitment or advertising services.

Submit your application through Uppsala University´s recruitment system, which can be accessed here:

https://www.uu.se/en/about-uu/join-us/details/?positionId=321159

 

Placement: Department of Immunology, Genetics and Pathology

Type of employment: Full time , Temporary position longer than 6 months

Pay: Individual salary

Number of positions: 1

Working hours: 100%

Town: Uppsala

County: Uppsala län

Country: Sweden

Union representative: Seko Universitetsklubben seko@uadm.uu.se
ST/TCO tco@fackorg.uu.se
Saco-rådet saco@uadm.uu.se

Number of reference: UFV-PA 2020/833

Last application date: 2020-04-06

 

Zebrafish Animal Technician II / 40 Hours / Day / BWH Center for Comparative

Medicine - (3121622)

Job Description

 

-Works under the general supervision of the Facility Manager and carries out the daily activities

of the aquatics facility network at Brigham and Women’s Hospital which include:

-Provide appropriate daily husbandry (feeding, tank exchanges and cleaning) to Zebra fish

and maintenance of the Zebra fish facility (equipment and facility sanitation) at 221

Longwood Ave and the BTM satellite facility


-Performs daily health checks and reports sick fish or high mortality events to veterinary

staff

-Monitors tanks required maintenance and contacts investigators for overdue tasks

-Performs facility cleaning and sanitation tasks

-Monitors facility for known pests and reports findings

-Keep daily records of facility parameters, documentation of breeding and stock

maintenance

-Monitor the water quality and the room environmental parameters and report out-of-range

findings.

-Feeds the zebrafish colony and prepares live for feeding the adult and juvenile fish at both

facilities.

-Uses the large-scale breeding chambers for generating embryos for study.

-Maintains inventory of feed and facilities supplies.

-Performs colony maintenance tasks for facility stock fish.

-Perform routine and non-routine maintenance and repairs at both facilities.

-Performs duties as assigned by the Facility Manager.

-Maintains and understands facilities SOPs and SOP manual.

-Trains new laboratory personnel on the proper use of the zebrafish facility, specialized

research protocols, and proper handling of the zebrafish in the most humane manner and

in compliance with regulatory mandates.

-Conducts independent experiments and assist users with zebrafish husbandry.


Qualifications

BS or BA degree is required

1 year of work experience is preferred but equivalent combination of fish biology or aquatic

husbandry experience will be taken into consideration.


ADDITIONAL SKILLS/ABILITIES/COMPETENCIES REQUIRED:

Must be willing to work weekends

Ability to understand spoken English to follow basic safety instructions and take direction from

supervisors; communicate effectively in basic English

Must have good manual dexterity

Ability to quickly learn new techniques and work independently

Must demonstrate careful attention to detail

Ability to lift 20lbs


EEO Statement

Brigham and Women’s Hospital is an Equal Opportunity Employer. All qualified applicants will receive consideration

for employment without race, sex, color, religion, national origin, sexual orientation, protected veteran status, or on the

basis of disability.


Primary Location MA-Boston-BWH Longwood Medical Area

Work Locations

75 Francis Street

Boston 02115

Job Research - Other

Organization Brigham & Women's Hospital(BWH)

Schedule Full-time

Standard Hours: 40

Shift Day Job

Employee Status Regular

Recruiting Department: BWH Research Administration

Posting Date Mar 4, 2020

If you are interested, please send a cover letter and your CV by email to Dr. Eva Buys (contact information below) or apply to Job#3121622 at the BWH job website https://www.brighamandwomens.org/about-bwh/careers


Hirschsprung disease: Zebrafish modelling to study cellular interactions involved in ENS development

Hirschsprung disease (HSCR) is a rare congenital gastrointestinal malformation characterized by lack of the enteric nervous system (ENS), aganglionosis, in the distal gut. The aganglionic segment is often limited to the sigmoid colon and rectum. Current treatment for HSCR is surgical removal of the affected aganglionic region. However, surgical intervention does not prevent further complications for the patient, including enterocolitis, fecal incontinence or chronic constipation. The search for alternative therapy is therefore, warranted.


The current theory about the mechanism underlying the aganglionosis is that the ENS progenitors, the enteric neural crest cells (ENCCs), do not reach the distal part of the colon, due to migratory or proliferation problems during embryonic development. It is generally accepted that HSCR is an inherited (genetic) disease, and to date more than 20 genes have been identified to be involved in its development. Since most of these genes are expressed in the ENS, or have functions in the establishment of the enteric neuronal network, HSCR is believed to be a neuronal disease.


In this PhD project we aim to identify interactions between ENCCs and the surrounding (non-neuronal) cells in their environment, that are involved in the development of the ENS. The zebrafish will be used as a model organism in this project, as it has several advantages over other model organisms to study the development of the ENS. Zebrafish embryonic development occurs ex-utero and rather rapid (4 days), which makes them assessable to study. The transparency of their skin enables us to study cellular processes real-time in a living organism.


Experimental techniques include:

  • gene editing using CRISPR/Cas9

  • sanger sequencing

  • single cell RNA sequencing and bioinformatic analysis

  • in vivo imaging using fluorescent microscopy and time-lapse confocal microscopy

  • image analysis

  • immunohistochemistry


Requirements:

  • Master's degree in Biology, Molecular Life Sciences or Biomedical Science.

  • Hands on working experience in molecular biology and cell biology.

  • Experience and affinity with zebrafish handling (article 9 certified).

  • Enthusiastic team player.

  • Accurate.

  • Pro-active: you take initiative and have a strong interest in academic research.

  • Analytical and flexible.

  • Excellent knowledge of the English language (spoken and written).

  • Academic writing and presentation skills.


Conditions of employment

We offer a full-time PhD position for initially one year. After a positive evaluation of the progress of the thesis, personal capabilities and compatibility, the appointment will be extended by a further three years.


Erasmus MC - Clinical genetics

Distinguishes fundamental research to understand the mechanisms which cause hereditary diseases, translational research for a translation of knowledge and renewing technology.


We use state of the art methods for studying hereditary monogenic and polygenic disorders. Next Generation

Sequencing and functional studies play an important role in the unraveling of the diseases. For the functional

genetics, in vitro as well as in vivo models are used. We follow the most recent developments like the use of

induced pluripotent stem cells (so-called iPS-cells) from isolated skin cells of patients. Widely applied animal

models for the functional research are genetically modified mice and zebrafish. The functional work is performed


in close cooperation with the Functional Unit of the Diagnostic section.


The research will be performed at the Clinical genetics department of the Erasmus MC (Rotterdam, NL) in the GI genetics group, headed by Prof.dr. Robert Hofstra.

If you are interested, please send a letter of motivation and your CV by email to Dr. Laura Kuil 

Deadline: April 1st 2020. l.kuil@erasmusmc.nl

 

Post doc position to investigate how wound inflammation drives various aspects of tissue repair

A Wellcome Trust funded post-doc position is available to join a team of researchers in Bristol investigating how inflammation triggered by tissue damage impacts on all other cell lineages at the wound site to drive good and bad repair outcomes. Experiments will include live-imaging, molecular biology, FACS and epigenetic/transcriptomic/proteomic analysis of inflammatory cells in various zebrafish models of wound inflammation.

 

The successful applicant will join the lab of Professor Paul Martin. The role will involve the day-to-day running of the project, performing a range of research tasks, collaborating with other RAs, in particular, a sister post-doc funded on the same grant and also investigating wound inflammation in Drosophila. You will also assist with training and supervision of postgraduate members of the lab.

 

A PhD (awarded or imminent) in a biological or biomedical science is essential and the successful candidate should have prior knowledge of tissue repair/regeneration or inflammation and experience of live imaging or working with tissue samples. Previous experience working with zebrafish is highly desirable but not essential. The successful applicant will be highly organised, eager to learn new skills, ambitious, and able to interact with a broad range of colleagues.  The funds for this position are available for up to 5 years.

 

Apply For job description, further particulars, and to apply: https://tinyurl.com/v2t8d47

 

Post doc position investigating how circadian influences impact inflammatory cells during tissue repair

The successful candidate will join team of researchers in Bristol and Manchester investigating how circadian influences impact on inflammatory cells and other cell lineages at sites of tissue damage to regulate collagen deposition and wound repair in zebrafish. Experiments will include live-imaging, molecular biology, FACS and transcriptomic analysis. The work will contribute to a 5-year UKRI-BBSRC-funded sLoLa project entitled “Opportunities to modulate extracellular matrix secretion and assembly for long term health”. The primary “home” for this researcher will be the laboratories of Professor Paul Martin, Professor David Stephens, and Dr. Chrissy Hammond, Bristol, but they will be expected to undertake short-term secondments in other collaborative labs, including that of Professor Qing-Jun Meng in Manchester.

 

The successful applicant will join a close-knit and enthusiastic research team. The role will involve the day-to-day running of the project, performing a range of research tasks and assisting with training and supervision of postgraduate members of the lab.

 

A PhD (awarded or imminent) in a biological or biomedical science is essential and the successful candidate should have prior knowledge of tissue repair/regeneration or inflammation and experience of live imaging or working with tissue samples. Previous experience working with zebrafish is highly desirable but not essential. The successful applicant will be highly organised, eager to learn new skills and to interact with a broad range of colleagues. The funds for this position are available for up to 5 years.

 

Apply For job description, further particulars, and to apply: https://tinyurl.com/sng423z


Our recent fishy wound papers include:

Gurevich et al (2020) J. Cell Sci.

Van den Berg et al (2019) Cell Reports

Gurevich et al (2018) EMBO J.

Morris et al (2018) Dev. Biol.


Closing date for both positions Sunday 5th April 2020

Informal enquiries Please contact Professor Paul Martin, Paul.Martin@bristol.ac.uk

Martin lab website http://www.bristol.ac.uk/biochemistry/research/martin-group/

A two years postdoctoral fellowship funding from the Japan Society for the
Promotion of Science (JSPS) is now available for application (funding rate
is around 15%) in William TSE lab in the Faculty of Agriculture, Kyushu
University.  The laboratory was relocated to the band new campus two years
ago. The applicant will work with the PI on various zebrafish projects
(Craniofacial disease model/ Developmental toxicity)

The ideal candidate should have experience on cell culture and zebrafish
model, and will be familiar with immunohistochemistry, confocal microscopy
and basic molecular biology.  Prior experience with embryonic
microinjection and /or  genome editing  methods is a plus. Fresh Ph.D
graduate around the world is welcome.

Interested applicants should send an email with CV to William
(kftse@agr.kyushu-u.ac.jp) for future information and discussion. The
information of the PI could be found at

https://urldefense.com/v3/__http://hyoka.ofc.kyushu-u.ac.jp/search/details/K006247/english.html*Papers__;Iw!!C5qS4YX3!RhHa-eOGl_OMVGAI7Vk77noc-0KhV_hC5ev6jfBuaFxtT96OG_EKsKenOXMewYmP$

while the fellowship program could be found at
Standard 2-year program
https://urldefense.com/v3/__https://www.jsps.go.jp/english/e-ippan/index.html__;!!C5qS4YX3!RhHa-eOGl_OMVGAI7Vk77noc-0KhV_hC5ev6jfBuaFxtT96OG_EKsKenOTthRckq$

Short-team 1 year program for EU and North America
https://urldefense.com/v3/__https://www.jsps.go.jp/english/e-oubei-s/index.html__;!!C5qS4YX3!RhHa-eOGl_OMVGAI7Vk77noc-0KhV_hC5ev6jfBuaFxtT96OG_EKsKenOXsGglmv$

Light-driven circadian clock entrainment and DNA repair: Decoding transcriptional regulation by the D-box enhancer.

A PhD student position is available immediately in the Chronobiology lab of Nicholas S. Foulkes at the Institute for Biological and Chemical Systems (Karlsruhe Institute of Technology, Germany, https://urldefense.com/v3/__https://bip.ibcs.kit.edu/foulkes.php__;!!C5qS4YX3!UGK3Iq55W1kh-Pj_oDRqMti0nXpKaDXVDffjXcRJYOPgF0Nir8iPutCPfVNiC3A3$ . This project that is supported jointly by DFG (Germany) and NSFC (China) funding involves a close collaboration with the laboratory of Prof. Han Wang at the University of Soochow, China (https://urldefense.com/v3/__https://zfin.org/ZDB-LAB-__;!!C5qS4YX3!UGK3Iq55W1kh-Pj_oDRqMti0nXpKaDXVDffjXcRJYOPgF0Nir8iPutCPfSgjWcec$  150413-1). The position is available for 1+2 years with remuneration based on the Collective Agreement for the Public Service Sector. The application process will remain open until the position is filled. Applications from disabled persons are welcome. The successful candidate will also be enrolled in the BioInterfaces International Graduate School (BIF-IGS).

In fish, unlike mammals, direct exposure of cells, tissues and even cell cultures to visible light induces the transcription of a subset of clock genes and consequently entrains the local circadian clocks. Many genes involved in DNA repair are also induced upon direct light exposure. We have demonstrated that the expression of light-regulated genes is also induced upon exposure to UV as well as the oxidative stress associated with exposure to sunlight. Importantly, these sunlight-regulated genes all share so-called D-box enhancer elements in their promoters. We aim i) to pinpoint the D-box binding transcription factors which direct sunlight-induced gene expression in zebrafish, ii) to explore their role in regulating the circadian clock and DNA repair and iii) to understand how evolution in complete darkness has affected these factors in blind cavefish. We are currently generating a zebrafish panel where each of a set of 13 D-box-binding transcription factors is either inactivated or inducibly over-expressed as well as establishing cell cultures from each line. We will study the circadian clock, DNA repair and the light-induced transcriptome in these lines and also examine the function of key D-box-binding factors in blind cavefish.

The Institute for Biological and Chemical Systems at the Karlsruhe Institute of Technology, hosts the European Zebrafish Resource Center (https://urldefense.com/v3/__https://www.ezrc.kit.edu__;!!C5qS4YX3!UGK3Iq55W1kh-Pj_oDRqMti0nXpKaDXVDffjXcRJYOPgF0Nir8iPutCPfS32gJ8b$ ). The Foulkes lab has pioneered the use of cell lines derived from various fish species including zebrafish, medaka and also blind cavefish to study the circadian clock and so represents one of the best-equipped laboratories world-wide for establishing and studying these unique cell culture models. Importantly, this collaborative project will also involve exchange visits and close cooperation with the laboratory of Prof. Han Wang at the University of Soochow. The Wang lab has considerable expertise in generating genetic models for studying the zebrafish circadian clock and has been a major player in the use of zebrafish to explore various aspects of chronobiology.

The candidate should hold an M.Sc. in biology (or equivalent) and ideally have experience in molecular biology techniques, cell and tissue culture as well as working with zebrafish. Some experience in bioinformatics would also be advantageous. The ideal candidate should be able to think independently, have excellent technical and organisational skills, have enthusiasm for team work and be fluent in English.

Please send your application to Prof. Nicholas S. Foulkes (nicholas.foulkes@kit.edu). The application should be sent as a single PDF. See https://urldefense.com/v3/__http://www.bif-igs.kit.edu/125.php__;!!C5qS4YX3!UGK3Iq55W1kh-Pj_oDRqMti0nXpKaDXVDffjXcRJYOPgF0Nir8iPutCPfa9uCrC8$  for details about documents that will ultimately be required to complete the application.

A postdoctoral fellow position is available immediately in the Lien Lab (Heart Regeneration Laboratory) at Children’s Hospital Los Angeles. We are looking for a hard-working and passionate candidate interested in using zebrafish, stem cell/organ culture, and/or mice to study molecular mechanisms of heart development, disease, and regeneration. We are focusing on the molecular mechanisms of coronary and lymphatic vessel formation and how these vessels affect cardiomyocytes. The candidate will analyze gene expression of different cell types and compare mutants with controls using single cell RNAseq and molecular imaging and generate and characterize CRISPR mutants of candidate genes involved in coronary and lymphatic vessel development and determine their roles in heart regeneration.

 

A strong background in molecular biology, confocal imaging, and/or cell culture is required. Previous experience with generating transgenic and CRISPR mutant zebrafish is preferred but not required. Experience in (micro) surgery/dissection in mouse or zebrafish and in situ hybridization is also preferred but not required. A Ph.D. degree is required.

 

We offer competitive salary and excellent benefits. Interested candidates should submit their CV and names of 3 references.

 

See the links below for details of our research and publications:

https://keck.usc.edu/faculty/ching-ling-lien/

https://www.ncbi.nlm.nih.gov/pubmed/31702553

https://www.ncbi.nlm.nih.gov/pubmed/26017769

https://www.ncbi.nlm.nih.gov/pubmed/22262006

https://www.ncbi.nlm.nih.gov/pubmed/20858732

https://careers.regeneron.com/job/REGEA002618058BR5080/Scientist-Zebrafish-Core


Position Summary

Scientist Zebrafish Core

Regeneron Pharmaceuticals, Inc.

Tarrytown, NY, United States

Requisition Number: 18058BR

Employment Type: Regular Employee


Known for its scientific and operational excellence, Regeneron is a leading science-based biopharmaceutical company that discovers, invents, develops, manufactures, and commercializes medicines for the treatment of serious medical conditions. Regeneron commercializes medicines for eye diseases, high LDL-cholesterol, atopic dermatitis and a rare inflammatory condition and has product candidates in development in other areas of high unmet medical need, including rheumatoid arthritis, asthma, pain, cancer and infectious diseases.

Summary:
We are interested in establishing a small genetics core group, using zebrafish as a model organism.
We are seeking a highly motivated and experienced scientist to help establish this group, and to drive our discovery and development efforts pertaining to mechanisms induced from in vivo biological experiments, applying target and therapeutic questions to the zebrafish model. It will be necessary to help establish and guide a small group of people to work collaboratively in developing the vision for future studies. Specific work will be around developing genetic models for testing of experimental therapeutics and for mechanistic biology studies, by also establishing innovative ways to study these models..
The secondary objective of this role is to provide outstanding scientific and people leadership, in addition to planning and management of in vivo, pre-clinical science, to a new team of researchers focused on target discovery and validation efforts across a wide range of areas in the various therapeutic areas, by applying the zebrafish approach. This role includes overseeing the non-clinical evaluation of drug candidates in animal models; establishing and validating animal models that are relevant for understanding mechanisms of aging, cardiovascular disease, neurological disease, fibrosis, etc that may give rise to, or create a permissive environment for, human clinically-relevant disorders. The team has access to REGN’s industry leading and proprietary technologies for target validation (VelociGene and VelociMouse), antibody generation (VelocImmune) and protein production (VelociMab) to support rapid target discovery and validation efforts.

Responsibilities:

o Provides scientific leadership in understanding new biology and mechanisms of vulnerability which can be tested and modelled in zebrafish.
o Provides strategic and innovative scientific leadership to achieve the Core Zebrafish goals in conjunction with Sr. Scientific management input and direction
o Manages and coordinates non-PhD scientific staff in the execution and design of experiments to discover and validate new targets within the various therapeutic realms, including establishing aged pre-clinical models, and disease models.
o Has primary and direct responsibility for evaluating the results of ongoing studies and sets clear priorities across the core group, to progress the best drug targets and candidates into the clinic
o Interacts with and presents to other groups in Discovery Research and Sr. Scientific management to inform them of progress, invite collaboration and to provide an in-depth review of the science
o Works collaboratively and openly invites scientific questioning from peers and the TFA teams alike
o Provides high quality and timely reports to support pre-IND and post IND activities and /or government agencies as needed
o Develops scientific team members in their ability to evaluate data critically, design experiments, present data in audience appropriate ways, and to ready our next generation of scientific talent for potential broader scientific and/or people leadership roles. Contributes to the REGN post doc program as needed/
o Establishes and leads extramural collaborations
o Writes and/or supports writing of manuscripts for publication in peer reviewed journals by the team
o Drives scientific rigor and innovation throughout the TFAs through keeping current on scientific topics and discoveries through literature review and conference attendance; ensures that the team does the same and new data and approaches are incorporated into the TFAs as appropriate

Requirements:
This position requires a minimum of a PhD/MD with at least 3 years of relevant academic and /or industry experience leading teams, focused on using zebrafish as a preclinical model. Some prior experience in muscle disease is helpful but not required. Some experience in industry is preferred. A proven track record of innovation via discoveries and publications that underscore his/her ability to understand the biology and mechanisms of vulnerability to age-related signaling changes.
The ideal candidate will have in-depth in vivo technical expertise, be hardworking, flexible, innovative and able to function effectively in a fast-paced environment. They will also have excellent communication skills and be able to work well in a team.

This is an opportunity to join our select team that is already leading the way in the Pharmaceutical/Biotech industry. Apply today and learn more about Regeneron’s unwavering commitment to combining good science & good business.

To all agencies: Please, no phone calls or emails to any employee of Regeneron about this opening. All resumes submitted by search firms/employment agencies to any employee at Regeneron via-email, the internet or in any form and/or method will be deemed the sole property of Regeneron, unless such search firms/employment agencies were engaged by Regeneron for this position and a valid agreement with Regeneron is in place. In the event a candidate who was submitted outside of the Regeneron agency engagement process is hired, no fee or payment of any kind will be paid.

Regeneron is an equal opportunity employer and all qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, sexual orientation, gender identity, disability status, protected veteran status, or any other characteristic protected by law.



University of Nebraska at Omaha

Tenure-Track Faculty Position in Neuroscience

College of Arts & Sciences

The Neuroscience Program at the University of Nebraska at Omaha (UNO) invites applications for a tenure-track Assistant Professor position. The preferred start date is August 2020, with a possible start date of January 2021. We seek applicants who are broadly trained and primarily interested in teaching. Undergraduate courses may include (but are not limited to) Introduction to Neuroscience, Neuroscience Methods, Statistics, Behavioral Neuroscience, Molecular and Cellular Neurobiology, and Advanced Neuroscience Laboratory. Coursework in the candidate’s area of expertise will be welcome. A molecular/cellular emphasis with a background in areas such as neuroimmunology, neuropharmacology, developmental, clinical and/or systems neuroscience are particularly welcome. As a faculty member, the successful applicant will teach three courses per semester, and receive a quarter-time research assignment. The successful applicant will be expected to mentor undergraduates in research and will have access to training graduate students at the Master’s and Ph.D. levels. Research expectations include applying for grants and funding. Tenure home will be the Psychology Department.


Candidates must have completed an earned doctorate in Neuroscience or a related field prior to the start date of employment. Area of specialization within Neuroscience is open. The ideal candidate will have a strong background of excellence in teaching with demonstrated commitments to supporting a diverse undergraduate student population.


The successful candidate will become part of a dynamic and growing program in the College of Arts & Sciences that is actively engaged with others on the campus as well as community partners. The university and department have a strong commitment to diversity in its varied forms and to fostering an inclusive and welcoming learning environment where students, faculty and staff thrive and succeed. With a metropolitan mission, UNO serves a diverse student body with a large number of first generation college students and is also actively engaged with the community in myriad ways. For more information about UNO’s Neuroscience program, please visit https://www.unomaha.edu/college-of-arts-and-sciences/neuroscience/academics/index.php


Please visit the UNO careers website at https://unomaha.peopleadmin.com to apply. Create your account, and search for the Tenure-track Assistant Professor in Neuroscience posting. Your online application should include a cover letter and curriculum vitae. Statements of teaching experience and goals, experience and dedication toward diversity and inclusion, research interests and goals, teaching evaluations, and optional supplemental information such as copies of publications (up to 3) should be attached as a single file in the portfolio area of the application. Include names and contact information of three references. Your references will be requested by the system to submit letters of recommendation electronically. We begin the review of applicants on March 17, 2020 and will continue until the position is filled. Please address questions to Dr. Suzanne Sollars at ssollars@unomaha.edu.


The University of Nebraska does not discriminate based on race, color, ethnicity, national origin, sex, pregnancy, sexual orientation, gender identity, religion, disability, age, genetic information, veteran status, marital status, and/or political affiliation in its programs, activities, or employment. UNO is a VEVRAA Federal Contractor and an E-Verify employer.

Postdoctoral position in the Lawson Lab - Modeling vascular malformations using the zebrafish


A postdoctoral position is available in the Lawson Lab at the University of Massachusetts Medical School to establish zebrafish models for vascular malformations. This project is focused on modeling the R183Q mutation in the G-protein, GNAQ, which is causative for Sturge-Weber syndrome (SWS). This work is part of a collaborative effort with Dr. Joyce Bischoff at Boston Children's Hospital and provides the opportunity to work in a multidisciplinary team focused on identifying developmental mechanisms of SWS progression and logical therapeutic targets. The project in the Lawson Lab will focus on establishing GNAQ R183Q knock-in zebrafish followed by detailed cellular and molecular characterization of the resulting phenotypes. Follow-up studies will include application of small molecule screening to identify therapeutic leads using the zebrafish model.


The ideal candidate must have experience in molecular biology as documented through their publication record. Prior expertise in the application of genome editing is welcome and experience with model organisms is preferred.  Applicants must otherwise be highly motivated and technically accomplished as documented by exceptional publication record.


Please send or email CV and reprints of relevant publications to the contact information below.  Applicants accepted for initial consideration will be subsequently contacted for letters of reference.


Nathan D. Lawson, PhD

Professor

Department of Molecular, Cell, and Cancer Biology

University of Massachusetts Medical School

Lazare Research Building, Room 617

364 Plantation Street

Worcester, MA 01605

e-mail: nathan.lawson@umassmed.edu

website: lawsonlab.umassmed.edu

Twitter: @LawsonZFLab