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Assays Tested

Assay

Prep

Worked

Notes

immunohistochemistry

yes

from ZFIN curation

Notes

  • Imported from ZFIN Antibody page Ab1-3G10
  • The antibody (F69-3G10 clone) reacts with heparan sulfate neo-epitope 3G10, generated by digesting heparan sulfate with heparitinase I (Heparinase III) from Flovobacterium heparinum (EC 4.2.2.8). The desaturated hexuronate (glucuronate) that is present at the non-reducing end of the heparan sulfate fragments created by the enzyme is critical for the reactivity of the antibody. The 3G10 antibody dose not react with oligosaccharides generated from chondroitin sulfates with bacterial chondroitinase ABC or AC, or generated from heparan sulfate with heparinase (Heparinase I): EC 4.2.2.7). (ZFIN Staff)
  • Citations for Ab1-3G10 at ZFIN
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