Assays Tested
Assay |
Prep |
Worked |
Notes |
|---|---|---|---|
immunohistochemistry | yes | from ZFIN curation | |
western blot | yes | from ZFIN curation |
Notes
- Imported from ZFIN Antibody page Ab1-fscn2
- Lin-Jones and Burnside (2007) generated fascin 2 antibodies using maltose-binding protein (MBP) fusions to Xenopus Fscn2, Danio rerio Fscn2A, and Danio rerio Fscn2B were expressed in BL21 cells and purified with amylose resin. A mixture of the three purified proteins was injected into rabbits to produce polyclonal antisera (Bethyl Laboratories, Montgomery, TX). (Lin-Jones, J., and Burnside, B.)
- Citations for Ab1-fscn2 at ZFIN