Position Type: Postdoctoral Position at the NIH
Position Title: OPTIMIZING PRECISE GENOME-EDITING METHODS IN ZEBRAFISH
The NICHD Zebrafish Core and NICHD Molecular Genomics Core are seeking a postdoctoral researcher to commence no later than September 2018 on a joint project to optimize precise genome-editing methods in zebrafish. In the first phase under Dr. Benjamin Feldman’s mentorship at the NICHD Zebrafish Core, previously described CRISPR/Cas9-based strategies for generating amino-acid coding substitutions in endogenous genes will be applied to loci with orthology to human disease-causing genes under study in NICHD’s Division of Intramural Research. In the second phase under Dr. Steven Coon’s mentorship at the NICHD Molecular Genomics Core, frequency of targeted alterations for the above methods and loci will be compared using NextGen sequencing. In a third phase working with both cores, a NextGen-sequencing based method for economically identifying infrequent germ-line transmitters will be developed and potential zebrafish disease models will be recovered. The NICHD and NIH offer an exceptional postdoctoral training environment through its collegial, supportive and rigorous approach to scientific research, its access to state-of-art equipment and resources, and its world-class seminar series.
This position requires an individual with a recent PhD and substantial experience and skills in (1) experimental design and troubleshooting, (2) molecular biology (cloning, PCR, etc.), (3) microscopy and fine-scale manipulation (needed for microinjection and handling of zebrafish embryos) and (4) bioinformatic and computational analysis of DNA sequence. Experience working with a small animal model is also desirable, though not essential.
Please email a CV, the names of three references, and a cover letter briefly describing research experience, interests, and career goals to the contact information below.
Benjamin Feldman, Ph.D.
Director of the NICHD Zebrafish Core
Eunice Kennedy Shriver National Institute for Child Health and Human Development (NICHD)
Bethesda, MD 20892
Employer Name: EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE FOR CHILD HEALTH AND HUMAN DEVELOPMENT
Position Location: Main NIH campus, Bethesda, MD
Application Deadline: July 7th, 2018
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