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Antibody Name:

Ab1-asic4b from the ZFIN antibody database.

Other names, clone ids, catalog ids etc.

anti-zASIC4.2 , anti-zebrafish ASIC4b , asic4b

Antibody Registry ID.

Does it work on zebrafish?

yes

Host organism

Rabbit

Immunogen organism

Zebrafish

Antibody isotype

Antibody type

polyclonal

Anatomical structures recognized
(use terms from the ZFIN Anatomical Ontology)

gill; gill filament vH ionocyte; gill lamella; vH ionocyte

Recognized target molecules (gene names, domains, epitopes ...)

asic4b  

Suppliers

Assays Tested

Assay

Prep

Worked

Notes

immunohistochemistry

yes

from ZFIN curation

western blot

yes

from ZFIN curation

Notes

  • Imported from ZFIN Antibody page Ab1-asic4b
  • No notes imported.
  • An anti-zebrafish ASIC4b (zASIC4.2) polyclonal antibody against two peptides corresponding to regions near the NH2 terminus (aa 146-160; PKSRKGHRPSELQYP) and the COOH terminus (aa 519-533; CFEEVKVKAANDVAQP) of ASIC4.2 protein (accession no. Q708S3.1) was custom-made by 21st Century Biochemicals (Marlboro, MA). The polyserum was affinity-purified against each of these peptides independently. (Dymowska, A.K., Schultz, A.G., Blair, S.D., Chamot, D., Goss, G.G.)
  • Citations for Ab1-asic4b at ZFIN