4. If the gene is new to zebrafish but has a human or mouse ortholog, check the Human Nomenclature Database or the Mouse Genome Informatics resources for the name and symbol used in those species:
Human and mouse gene names in these two resources are approved by nomenclature committees.
HUGO Gene Nomenclature Committee: http://www.genenames.org/
The Genome Database: http://gdbwww.gdb.org/gdb/
- Provide a sequence accession number if the sequence is public in an on-line sequence database. It is very helpful if you include the amino acid sequence, nucleotide sequence, or both with your request if they are not publicly available.
- If you have mapped the gene on a mapping panel, provide the map position and on which mapping panel the gene is mapped. Preferably indicate its location with respect to the two nearest flanking "z" markers (MGH SSLPs - http://zebrafish.mgh.harvard.edu/). If If you have placed the gene on the Sanger Institute's Ensembl contig (http://www.ensembl.org/Danio_rerio/), please provide the coordinates.
- If you believe the zebrafish gene is the ortholog of a gene in another species, provide the evidence (amino acid sequence comparison, nucleotide sequence comparison, conserved synteny, probe cross hybridization, etc.).
- If possible, please provide a phylogenetic tree or description of the relationships between your zebrafish gene and orthologous or related genes in other species.
- Include any other information or commentary you view as pertinent in assigning proper gene nomenclature.