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Assays Tested
Assay | Prep | Worked | Notes |
|---|---|---|---|
western blot | yes | from ZFIN curation |
Notes
- Imported from ZFIN Antibody page Ab1-camk1da
- GST-fused CaMKId-LL(334-433) was expressed in E. coli and CaMKId-LL (334-433) was purified by glutathione Sepharose 4B and PreScission protease, a specific antibody against CaMKId-LL was raised by immunizing a rabbit with purified CaMKId-LL(334-433) as an antigen. The anti-CaMKId-LL antibody was purified by affinity chromatography using CNBr-activated Sepharose 4B (GE Healthcare Bio-Sciences) coupled by CaMKId-LL(334-433). (Senga, Y., Yoshioka, K., Kameshita, I., and Sueyoshi, N.)
- Citations for Ab1-camk1da at ZFIN