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{show-to:users=@authenticated} {add-page:template=antibody|parent=@home|labels=community_antibody|allowRename=true|live=true}Create New Antibody{add-page} {show-to} {link-to:add comment}Add Comment{link-to} {table-plus:columnAttributes=style=width:20%|sortIcon=true} || Antibody Name: | [Ab1-fscn2|http://zfin.org/action/marker/view/ZDB-ATB-110901-1] from the [ZFIN antibody database|http://zfin.org/action/antibody/antibody-search]. || || Other names, clone ids, catalog ids etc. | fscn2 {hidden-data}ab1-fscn2{hidden-data} || || Does it work on zebrafish\? | yes || || Host organism | Rabbit || || Immunogen organism| Zebrafish || || Antibody isotype | || || Antibody type | polyclonal || || Anatomical structures recognized \\ (use terms from the [ZFIN Anatomical Ontology|http://zfin.org/action/anatomy/anatomy-search]) | [eye|http://zfin.org//action/anatomy/term-detail?anatomyItem.zdbID=anatomy-view/ZFA:0000107] [hair cell anterior macula|http://zfin.org//action/anatomy/term-detail?anatomyItem.zdbID=anatomy-view/ZFA:0000678] [macula lagena|http://zfin.org//action/anatomy/term-detail?anatomyItem.zdbID=anatomy-view/ZFA:0000116] [posterior crista|http://zfin.org//action/anatomy/term-detail?anatomyItem.zdbID=anatomy-view/ZFA:0000566] [retinal cone cell|http://zfin.org//action/anatomy/term-detail?anatomyItem.zdbID=anatomy-view/ZFA:0009262] || || Recognized target molecules (gene names, domains, epitopes ...) | {text-data:RecognizedTargetMolecules}{text-data} || || Recognized ZFIN genes \\ current ZFIN-gene symbols only [ZFIN Gene Search|http://zfin.org/cgi-bin/webdriver?MIval=aa-newmrkrselect.apg] | [_fscn2a_|http://zfin.org/cgi-bin/webdrivermarker/view/ZDB-GENE-110901-1|fascin homolog 2, actin-bundling protein, retinal (Strongylocentrotus purpuratus) a] [_fscn2b_|http://zfin.org/cgi-bin/webdrivermarker/view/ZDB-GENE-040426-1740|fascin homolog 2, actin-bundling protein, retinal (Strongylocentrotus purpuratus) b] || || Supplier(s) | || {table-plus} h3. Assays Tested || Assay || Prep ||Worked || Notes || | immunohistochemistry | | yes | from [ZFIN curation|http://zfin.org/action/marker/view/ZDB-ATB-110901-1] | | western blot | | yes | from [ZFIN curation|http://zfin.org/action/marker/view/ZDB-ATB-110901-1] | h3. Notes * Imported from ZFIN Antibody page [Ab1-fscn2|http://zfin.org//action/marker/view/ZDB-ATB-110901-1|Ab1-fscn2] * Lin-Jones and Burnside (2007)generated fascin 2 antibodies using maltose-binding protein (MBP) fusions to Xenopus Fscn2, Danio rerio Fscn2A, and Danio rerio Fscn2B were expressed in BL21 cells and purified with amylose resin. A mixture of the three purified proteins was injected into rabbits to produce polyclonal antisera (Bethyl Laboratories, Montgomery, TX). ([Lin-Jones, J., and Burnside, B.|http://zfin.org/cgi-bin/webdriver?MIval=aa-pubview2.apg&OID=ZDB-PUB-110823-22|Retina-specific protein fascin 2 is an actin cross-linker associated with actin bundles in photoreceptor inner segments and calycal processes]) * [Citations for Ab1-fscn2 at ZFIN |http://zfin.org//action/antibody/publication-list?orderBy=author&antibody.zdbID=ZDB-ATB-110901-1] |
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