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{show-to:users=@authenticated} {add-page:template=antibody|parent=@home|labels=community_antibody|allowRename=true|live=true}Create New Antibody{add-page} {show-to} {link-to:add comment}Add Comment{link-to} {table-plus:columnAttributes=style=width:20%|sortIcon=true} || Antibody Name: | [Ab1-fscn2|http://zfin.org/action/marker/view/ZDB-ATB-110901-1] from the [ZFIN antibody database|http://zfin.org/action/antibody/antibody-search]. || || Other names, clone ids, catalog ids etc. | fscn2 {hidden-data}ab1-fscn2{hidden-data} || || Does it work on zebrafish\? | yes || || Host organism | Rabbit || || Immunogen organism| Zebrafish || || Antibody isotype | || || Antibody type | polyclonal || || Anatomical structures recognized \\ (use terms from the [ZFIN Anatomical Ontology|http://zfin.org/action/anatomy/anatomy-search]) | [eye|http://zfin.org//action/anatomy/anatomy-view/ZFA:0000107]  [hair cell anterior macula|http://zfin.org//action/anatomy/anatomy-view/ZFA:0000678]  [macula lagena|http://zfin.org//action/anatomy/anatomy-view/ZFA:0000116]  [posterior crista|http://zfin.org//action/anatomy/anatomy-view/ZFA:0000566]  [retinal cone cell|http://zfin.org//action/anatomy/anatomy-view/ZFA:0009262]   || || Recognized target molecules (gene names, domains, epitopes ...) | {text-data:RecognizedTargetMolecules}{text-data} || || Recognized ZFIN genes \\ current ZFIN-gene symbols only [ZFIN Gene Search|http://zfin.org/cgi-bin/webdriver?MIval=aa-newmrkrselect.apg] | [_fscn2a_|http://zfin.org/action/marker/view/ZDB-GENE-110901-1|fascin homolog 2, actin-bundling protein, retinal (Strongylocentrotus purpuratus) a]  [_fscn2b_|http://zfin.org/action/marker/view/ZDB-GENE-040426-1740|fascin homolog 2, actin-bundling protein, retinal (Strongylocentrotus purpuratus) b]   || || Supplier(s) | || {table-plus} h3. Assays Tested || Assay || Prep ||Worked || Notes || | immunohistochemistry | | yes | from [ZFIN curation|http://zfin.org/action/marker/view/ZDB-ATB-110901-1] | | western blot | | yes | from [ZFIN curation|http://zfin.org/action/marker/view/ZDB-ATB-110901-1] | h3. Notes * Imported from ZFIN Antibody page [Ab1-fscn2|http://zfin.org//action/marker/view/ZDB-ATB-110901-1|Ab1-fscn2] * Lin-Jones and Burnside (2007)generated fascin 2 antibodies using maltose-binding protein (MBP) fusions to Xenopus Fscn2, Danio rerio Fscn2A, and Danio rerio Fscn2B were expressed in BL21 cells and purified with amylose resin. A mixture of the three purified proteins was injected into rabbits to produce polyclonal antisera (Bethyl Laboratories, Montgomery, TX). ([Lin-Jones, J., and Burnside, B.|http://zfin.org/cgi-bin/webdriver?MIval=aa-pubview2.apg&OID=ZDB-PUB-110823-22|Retina-specific protein fascin 2 is an actin cross-linker associated with actin bundles in photoreceptor inner segments and calycal processes]) * [Citations for Ab1-fscn2 at ZFIN |http://zfin.org/action/antibody/antibody-publication-list?antibodyID=ZDB-ATB-110901-1]
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Antibody Name:

Ab1-fscn2 from the ZFIN antibody database.

Other names, clone ids, catalog ids etc.

fscn2

Antibody Registry ID.

Does it work on zebrafish?

yes

Host organism

Rabbit

Immunogen organism

Zebrafish

Antibody isotype

Antibody type

polyclonal

Anatomical structures recognized
(use terms from the ZFIN Anatomical Ontology)

eye; hair cell anterior macula stereocilium bundle; macula lagena stereocilium bundle; posterior crista stereocilium bundle; retinal cone cell photoreceptor inner segment

Recognized target molecules (gene names, domains, epitopes ...)

fscn2a  fscn2b  

Suppliers

Assays Tested

Assay

Prep

Worked

Notes

immunohistochemistry

yes

from ZFIN curation

western blot

yes

from ZFIN curation

Notes

  • Imported from ZFIN Antibody page Ab1-fscn2
  • Lin-Jones and Burnside (2007) generated fascin 2 antibodies using maltose-binding protein (MBP) fusions to Xenopus Fscn2, Danio rerio Fscn2A, and Danio rerio Fscn2B were expressed in BL21 cells and purified with amylose resin. A mixture of the three purified proteins was injected into rabbits to produce polyclonal antisera (Bethyl Laboratories, Montgomery, TX). (Lin-Jones, J., and Burnside, B.)
  • Citations for Ab1-fscn2 at ZFIN