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Antibody Name: | Ab1-oprd1b from the ZFIN antibody database. |
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Other names, clone ids, catalog ids etc. | oprd1b |
Does it work on zebrafish? | yes |
Host organism | Rabbit |
Immunogen organism | Zebrafish |
Antibody isotype | |
Antibody type | |
Anatomical structures recognized | brain; central nervous system; cerebellum; dorsal telencephalon; hypothalamus; inferior lobe; medulla oblongata; optic tectum; telencephalon |
Recognized target molecules (gene names, domains, epitopes ...) | |
Suppliers |
Assays Tested
Assay | Prep | Worked | Notes |
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immunohistochemistry | yes | from ZFIN curation |
Notes
- Imported from ZFIN Antibody page Ab1-oprd1b
- The 3′ DNA fragments corresponding to the nucleotides 1030–1122 of DOR2 (NM_21275), from zebrafish was amplified from cDNA by polymerase chain reaction (PCR) using primers flanked by EcoRI and XhoI sequences and cloned in the pGEX-6P-1 vector. These plasmids were expressed in bacteria in the presence of IPTG (0.1 mM) to generate recombinant proteins consisting of GST fused to the last 30 (I344-T373) amino acids of oprd1b. (Arévalo, J.C., Hernández-Jiménez, E., Jiménez-González, A., Torres-Valle, M., Iwasaki, R.S., López-Bellido, R., Vicente-García, C., Rodríguez, R.E.)
- Citations for Ab1-oprd1b at ZFIN