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4.3.1 Transgenic constructs

Construct Nomenclature

Tg(promoterregulatory sequence:genecoding sequence)

Tg indicates transgene. Within the parentheses, the most salient features of the transgene should be described. Brevity and clarity in the transgene name are favored, in general, over exhaustive detail. Regulatory sequences, which can be derived from either an enhancer or promoter, should be listed to the left of the colon, and coding sequences . In general, the regulatory sequence is named for the gene from which it was derived or the gene/transcript that it regulates. Coding sequences are placed to the right of the colon. Not all transgenic constructs will have both regulatory and coding elements, and in this case, the colon will not be used. In cases where a construct utilizes sequences from a named gene, it should contain the standard zebrafish lowercase symbol for that gene.  The entire transgene name should be italicized. 

For those cases where a specific transcript or transcript promoter of a gene is used, the transcript number or name should be used. It should be noted that the use of hyphens here is distinct from the use of hyphens in regulatory or coding sequence fusions as discussed below. The hyphen in transcript names is an integral part of the transcript name and demarcates the transcript number for a gene.

Enhancer trap, promoter trap, gene trap constructs: These all use the same nomenclature conventions as described for transgenic constructs, substituting Et, Pt, Gt as necessary.


     Example: Tg(pitx2-002:GFP) In this case an internal pitx2 gene promoter that generates the pitx2-002 transcript is driving expression of GFP.


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