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{add-page:template=antibody|parent=@home|labels=community_antibody|allowRename=true|live=true}Create New Antibody{add-page}
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|| Antibody Name: | [Ab1-fscn2|http://zfin.org/action/marker/view/ZDB-ATB-110901-1] from the [ZFIN antibody database|http://zfin.org/action/antibody/antibody-search]. ||
|| Other names, clone ids, catalog ids etc. | fscn2  {hidden-data}ab1-fscn2{hidden-data}  ||
|| Does it work on zebrafish\? | yes ||
|| Host organism | Rabbit ||
|| Immunogen organism| Zebrafish ||
|| Antibody isotype |  ||
|| Antibody type | polyclonal ||
|| Anatomical structures recognized \\
(use terms from the [ZFIN Anatomical Ontology|http://zfin.org/action/ontology/ontology-search]) | [eye|http://zfin.org//action/ontology/term-detail/ZFA:0000107]  [hair cell anterior macula|http://zfin.org//action/ontology/term-detail/ZFA:0000678]  [macula lagena|http://zfin.org//action/ontology/term-detail/ZFA:0000116]  [posterior crista|http://zfin.org//action/ontology/term-detail/ZFA:0000566]  [retinal cone cell|http://zfin.org//action/ontology/term-detail/ZFA:0009262]   ||
|| Recognized target molecules (gene names, domains, epitopes ...)  | {text-data:RecognizedTargetMolecules}{text-data} ||
|| Recognized ZFIN genes \\
current ZFIN-gene symbols only [ZFIN Gene Search|http://zfin.org/cgi-bin/webdriver?MIval=aa-newmrkrselect.apg] | [_fscn2a_|http://zfin.org/action/marker/view/ZDB-GENE-110901-1|fascin homolog 2, actin-bundling protein, retinal (Strongylocentrotus purpuratus) a]  [_fscn2b_|http://zfin.org/action/marker/view/ZDB-GENE-040426-1740|fascin homolog 2, actin-bundling protein, retinal (Strongylocentrotus purpuratus) b]   ||
|| Supplier(s) |  ||
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Assays

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Tested

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Assay

Prep

Worked

Notes

immunohistochemistry

 

yes

from ZFIN curation

western blot

 

yes

from ZFIN curation

Notes

  • Imported from ZFIN Antibody page Ab1-fscn2
  • Lin-Jones and Burnside (2007)generated fascin 2 antibodies using maltose-binding protein (MBP) fusions to Xenopus Fscn2, Danio rerio Fscn2A, and Danio rerio Fscn2B were
    expressed in BL21 cells and purified with amylose resin. A mixture of
    the three purified proteins was injected into rabbits to produce polyclonal
    antisera (Bethyl Laboratories, Montgomery, TX). (Lin-Jones, J., and Burnside, B.)
  • Citations for Ab1-fscn2 at ZFIN