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1μg linearized DNA
Transcription buffer (T3 or T7 RNA polymerase) 4 μl
NTP DIG RNA (Boehringer) 2 μl
RNAse inhibitor (35 units/μl) 1 μl
T3/T7 RNA polymerase (20 units/μl, Stratagene) 1 μl
Sterile water to 20 μl total