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116 mM NaCl
2.9 mM KCl
1.8 mM CaCl2
5.0 mM HEPES, pH 7.2.

High calcium:
116 mM NaCl
2.9 mM KCl
10 mM CaCl2
5 mM HEPES, pH 7.2.

Calcium free:
116 mM NaCl
2.9 mM KCl
5.0 mM HEPES, pH 7.2





  1. Does anyone know how long you can store ringer's solution? Do you store it at RT, at 4 or at -20 degrees celcius?

    Thanks in advance,


  2. From an online MSDS:

    Storage: Store in General Storage Area (Green Storage) with other items with no specific storage hazards. Store in a cool, dry, well-ventilated, locked store room away from incompatible materials.

    Stability: Stable under normal conditions of use and storage.
    Shelf life: Indefinite shelf life if stored properly

    From another site:

    Store at room temperature between 59 and 86 degrees F (15 to 30 degrees C) away from light and moisture.

  3. Dear Barbara,

    Thank you for this information. One more question: that it is stable, does that mean you can store it forever, or do I have to change it every 6 months? What would you recommend?

    Yours sincerely,


    1. Hi Suzanne,

      If it is stored according to the instructions, and you have no reason to believe there's been contamination, it can be stored and used forever.