GROWTH MEDIUM
L-15 (Sigma)
0.30 mg/ml glutamine
50 U/ml penicillin
0.05 mg/ml streptomycin
0.80 mM CaCl2
10% embryo extract
3% fetal calf serum
L-15 (Sigma)
0.30 mg/ml glutamine
50 U/ml penicillin
0.05 mg/ml streptomycin
0.80 mM CaCl2
10% embryo extract
3% fetal calf serum