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(Source: D. Frost and D. Sepich from Zebrafish Book 5th Edition)

  1. Place embryos into high calcium Ringer. Cut or pull into pieces using sharpened forceps or needles.
  2. Rinse by dipping into growth medium twice.
  3. Plate onto tissue culture dish in a single drop of medium with 5% serum (to help attachment).
  4. Incubate 4 hours at 28.5°C.
  5. Gently fill the dish with growth medium without serum.