FIFTH (and FINAL) Solicitation for NIH-funded Resource to Target Zebrafish Genes with Engineered Nucleases

We are writing again to solicit recommendations for appropriate genes
to target in our FIFTH (and FINAL) solicitation of zebrafish genes to
target with engineered nucleases. As in the previous (fourth) round,
we plan to construct 96 TALEN pairs. (Note that plasmids for the 96
pairs made for the fourth solicitation are nearly completed and will
be deposited with Addgene by the end of February.)

Each laboratory may submit up to TWO gene targets by completing an
Excel form that can be downloaded here: http://www.talengineering.org/download.htm.
Note that one gene recommendation should be placed on each of the
"Sheets" in the file. Place your first choice gene on the first sheet
and your second choice gene on the second sheet. Please note that ALL
required fields must be filled out - we will not accept or consider
any incomplete forms. For a gene to be considered appropriate for
this effort, we ask each recommender to verify on the form that
mutations in the gene of interest do not already exist and that
efforts to generate mutations in the recommended gene are not underway
elsewhere. VERY IMPORTANT NOTE: Each gene submitted MUST include its
Ensembl Gene ID (ENSDARG) the Ensembl Transcript ID (ENSDART), and the
gene name (e.g.-- Example: ENSDARG00000024771, ENSDART00000033574,
slc24a5). In the event that Ensembl has incorrectly annotated the
gene of interest, the full genomic sequence of your gene should be
included with exons denoted by upper case and introns in lower case.
Genes not correctly submitted in one of these formats will NOT be
considered for targeting.

Recommendations will be considered in the order in which they are
received. We will choose one gene for targeting from each lab until
we reach 96 gene targets. If we receive fewer than 96 targetable
"first choice" gene requests, we will then consider "second choice"
requests in the order in which we received the e-mails until we reach
96 gene targets.

Please e-mail your completed form to zebrafishzfs@zincfingers.org at
or after (and not before) 12:00 Noon (Eastern US time) Friday,
February 22, 2013. Requests received before this date/time will not
be considered. Gene recommendations will only be accepted via e-
mail. Note that recommendations from scientists at commercial
entities will not be accepted.

Keith Joung
Randall Peterson
Joanna Yeh
Jeffry Sander
Deepak Reyon
Jon Foley

Massachusetts General Hospital