Molecular Biology Scientist - Eugene, OR

Created by Jonathan Knight
Oct 15, 2019
3 min read

Our Company

NemaMetrix Inc. is a fast-paced, biotechnology company whose mission is to enable scientists and researchers around the world to better understand human health by offering a more affordable and accessible platform. We develop solutions for the genetic modification of C. elegans and D. rerio (Zebrafish) to act as human proxies for disease research and drug discovery and help our customers reveal new insights into the true nature of genetic mutations.  

NemaMetrix is headquartered in Eugene, Oregon with a satellite office located in Salt Lake City, Utah.


Role: Molecular Biology Scientist

Location: Eugene, OR

Overview:

Working as part of our Research & Development team, this role’s principal focus is to deliver the necessary biological constructs, such as plasmids, to downstream teams for the creation and ultimate analysis of transgenic C elegans and Zebrafish. The incumbent will oversee most of the day to day lab activities including scheduling, daily/weekly prioritization, data collection, reprocessing, training, development of and adherence to SOPs, maintenance requests, and related activities.

What You’ll be Doing:

  • Designing and generating constructs for CRISPR mediated insertion of disease relevant genetic mutations, transgenic reporters and protein tags into model organisms, primarily Zebrafish.

  • Applying standard and advanced molecular biology techniques for detecting precise edits in vivo (insertion & integration).

  • Generation and propagation of de novo genome edited animals.  

  • Identifying and troubleshooting innovative technologies to validate design and capture integration events.

  • Working independently and within a team to meet commercial project schedules, to optimize timelines and increase workflow of the engineering pipeline.


What You Bring to the Table: NemaMetrix is looking for individuals that possess a positive can-do attitude, are motivated by doing a job well, are adaptable and flexible, have a continuous improvement mindset and will thrive in a fast-paced collaborative and diverse environment. In addition, successful candidates will have the following:


Required Qualifications

  • PhD in Molecular Biology or a related field; Or BS or MS with 8+ years of experience.

  • Hands on experience with CRISPR genome editing methodologies, specifically with zebrafish.

  • Demonstrated success with site directed mutagenesis and homology driven repair.

  • Functional knowledge of advanced homology based cloning methods such as Gibson Assembly, Golden Gate or Infusion.

  • Proficient in standard molecular and biochemistry analysis.

  • Ability to design and build plasmids and transgenic constructs.

  • In-vitro transcription and RNA purification.

  • DNA and RNA Sequence Analysis.

  • Zebrafish Husbandry and Genotyping experience.


Preferred Qualifications

  • Proficiency with Morpholino design and microinjection

  • Familiarity with the tol2 transposon system

  • Molecular Phenotyping using qPCR analysis

  • Experience with Western Blotting, specifically in Zebrafish

  • Ability to design and interpret High Resolution Melting (HRM) analyses

  • Clinical Genetics experience


Perks of Working with Us

In addition to our friendly and collegial staff, NemaMetrix offers a benefits package that includes:

  • Medical, Dental, Vision and Life/AD&D insurance

  • 401k plan

  • Paid Time Off

  • Flexible work schedules

  • Employee Events – including Summer BBQs, community service and holiday related events.

  • Dog friendly work environment in our Eugene, OR office


Our Commitment to You

NemaMetrix is an equal opportunity employer. NemaMetrix recruits, employs, trains, compensates and promotes individuals regardless of race, religion, color, national origin, sex, disability, age, sexual orientation, gender identity, veteran status, and any other protected category as required by applicable law.

Our dedication to promoting diversity, multiculturalism, and inclusion is clearly reflected in our content and across all our efforts. Diversity is more than a commitment at NemaMetrix—it is the foundation of what we do. We are fully focused on equality and believe deeply in diversity of all the fascinating characteristics that make us different.