Blog from March, 2019

RESEARCH TECHNICIAN II IN ZEBRAFISH MICROBIOME LAB

Department of Molecular Genetics and Microbiology

Duke University School of Medicine

 

Apply here: http://tinyurl.com/y47aagzg

 

Occupational Summary:

The Department of Molecular Genetics and Microbiology at Duke University Medical Center is seeking a research technician with near-immediate availability. This position will involve performing a variety of technical and research duties to support the laboratory of Dr. John Rawls (https://sites.duke.edu/rawlslab/) and the Duke University School of Medicine Zebrafish Core Facility (http://sites.duke.edu/zebrafishfacility/).

 

Description of research activities:

The successful candidate will have the demonstrated ability to perform, or be willing to learn, the following tasks in a research laboratory:

 

  1. Technical duties conducted routinely in an animal physiology laboratory such as molecular cloning, DNA and RNA extraction, DNA sequencing, genotyping, gene expression studies, microscopy, imaging, microinjection, animal handling and manipulation. The candidate will be expected to document procedures and collect data in an organized, complete, and accurate fashion.
  2. Laboratory maintenance duties including the preparation of culture media, chemicals, reagents and solutions, as well as assisting with maintaining equipment and lab reagent inventories.
  3. Support duties include assisting faculty, postdocs and students in the development and execution of new research procedures and techniques.

 

The successful candidate will also have the demonstrated ability to perform, or be willing to learn, the following tasks in a zebrafish aquaculture facility:

 

  1. Zebrafish care and maintenance duties include providing daily fish feedings, caring for Artemia cultures, checking fish health daily, transferring embryos and providing care to nursery racks.
  2. Facility maintenance duties include recording daily water parameters and reviewing for abnormal readings, handling basic needs of the facility during weekends, changing system filters, cleaning, maintenance, and organization of tanks and aquaculture components, assisting facility management in resolving any problems in facility operation, supporting facility sanitation protocols, and assisting in writing and maintaining compliance with IACUC protocols.

 

Preferred qualifications:

Education/Training: Preference of a Bachelor’s degree in biology, marine biology, aquaculture, zoology, or other related fields.

Experience: Previous laboratory experience is preferred but not required. Individuals with zebrafish or other aquaculture experience will be given priority, and candidates with relevant experience in other animal models are also encouraged to apply.

 

Duke University is an Affirmative Action/Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, gender, gender expression, gender identity, genetic information, national origin, race, religion, sex, sexual orientation, or veteran status.

 

Duke aspires to create a community built on collaboration, innovation, creativity, and belonging. Our collective success depends on the robust exchange of ideas—an exchange that is best when the rich diversity of our perspectives, backgrounds, and experiences flourishes. To achieve this exchange, it is essential that all members of the community feel secure and welcome, that the contributions of all individuals are respected, and that all voices are heard. All members of our community have a responsibility to uphold these values.

 

Essential Physical Job Functions: Certain jobs at Duke University and Duke University Health System may include essential job functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.

 

 

A postdoctoral position is available in the Lawson Lab at the University of Massachusetts Medical School. The Lawson Lab takes advantage of the zebrafish as a model system to study multiple aspects of vascular and lymphatic development. Current areas of interests include: origins of endothelial and mural cell heterogeneity, developing zebrafish as a platform to identify small molecule therapeutics, and optimization of genome editing approaches for targeted gene knock-in in zebrafish. Our efforts are supported by a recently awarded R35 grant from NHLBI that provides generous support for the next 6 years. An overview of our past and ongoing research can be found at our website (http://lawsonlab.umassmed.edu).   

Applicants must be highly motivated and technically accomplished as documented by exceptional publication record.  Candidates must have demonstrated experience in molecular biology. Experience with developmental model systems is preferred. 

Please send or email CV and reprints of relevant publications to the contact information below.  Applicants accepted for initial consideration will be subsequently contacted for letters of reference.

Nathan D. Lawson, PhD
Professor
Department of Molecular, Cell, and Cancer Biology
University of Massachusetts Medical School
Lazare Research Building, Room 617
364 Plantation Street
Worcester, MA 01605
e-mail: nathan.lawson@umassmed.edu

The McMenamin Lab at Boston College is looking for a qualified and highly motivated person to serve as a technician. Our lab uses highly interdisciplinary approaches answer fundamental questions about skeleton development, the molecular and endocrine regulation of developmental coordination, and the evolution of life history variation in vertebrates. We use the zebrafish model to study the impacts of thyroid hormone on bone morphogenesis, patterning and development, see https://sites.google.com/a/bc.edu/mcmenamin-lab/. The McMenamin Lab is a diverse environment and we particularly encourage applications from women, researchers of color, LGBTQ individuals, and people from disadvantaged backgrounds.
 

Primary responsibilities:

• Helping to manage and maintain our zebrafish colony; overseeing the schedules for feeding and husbandry; breeding and maintaining fish lines; interfacing with the animal care facility staff

• Helping to manage and maintain our molecular lab; ordering reagents and other supplies and maintaining laboratory equipment; lab administration, including lab safety, reporting and record keeping

• Training and supervising students in the lab and in the animal care facility

• Ideally, performing bench work and microscopy; contributing to data analysis and interpretation

Requirements:

• Minimum of a bachelor’s degree in Biology or a related field

• 2 or more years laboratory experience

• Working knowledge of fundamental biological concepts and ability to apply knowledge in varied situations

• Excellent communications skills and attention to detail; ability to work both independently and as part of a team

Preferred characteristics:

• Experience with zebrafish or other aquatic system, fluorescent microscopy and molecular biology

• Experience as a supervisor and manager

• Highly interested in our research program and ability to contribute as a productive researcher

Position:

Full time position, salary will be commensurate with experience. Initially a 6 month appointment, to be renewed as appropriate.

Application:

Please submit a single PDF of (1) a cover letter that includes a description of research interests and experience, (2) CV or resume, including undergraduate relevant coursework and GPA (3) a list of 3 or more professional references to mcmenams@bc.edu. Applications submitted by April 12, 2019 will receive priority consideration, but position is open until filled. Preferred start date will be in May or sooner.

Boston College conducts background checks as part of the hiring process. Boston College is an Affirmative Action/Equal Opportunity Employer and does not discriminate on the basis of any legally protected category including disability and protected veteran status. To learn more about how BC supports diversity and inclusion throughout the university please visit the Office for Institutional Diversity at http://www.bc.edu/offices/diversity.

The Parichy Lab (dparichy.as.virginia.edu/) at University of Virginia seeks a Research Assistant or Research Associate for studies of stem cell and regenerative biology employing methods of developmental genetics and genomics, using both zebrafish and amphibian systems.

The laboratory has dedicated, state of the art facilities for molecular biology, genomics, transgenesis, CRISPR/Cas9 screening, and high resolution time-lapse imaging. The group is affiliated with Departments of Biology and Cell Biology, each having highly interactive faculty and trainees housed in outstanding facilities.

The position includes numerous opportunities for professional growth, mentoring and outreach.

Applicants must have at least 2 years of research experience and skills in molecular biology. Experience in genomics (e.g., RNA-seq, next generation sequencing library preparation), bioinformatics (especially in R), microscopy, or developmental model organisms are desirable. Applicants having or anticipating bachelor’s, master’s or doctoral degrees are welcome.

For more information contact Dr. David Parichy and include a cover letter with current CV.

Please send CV, cover letter and references to: 

David M. Parichy, Professor
Department of Biology and Department of Cell Biology
University of Virginia
dparichy@virginia.edu

We are seeking a highly motivated, organized and enthusiastic postdoctoral fellow to study embryonic kidney development and its relationship to developmental kidney diseases. We are interested in the role of Wnt signaling in shaping nephric tubules, utilizing Xenopus (frog) embryos and mammalian tissue culture as models. Current goals include: 1) Determining how junctions are formed during tubule formation; 2) Modeling how patient mutations result in human developmental kidney diseases; 3) Discovering novel components affecting nephron development; 4) Visualizing in vivo tube formation using advanced live imaging techniques; 5) Generating transgenic animals to visualize nephrogenesis in vivo

 

Current projects utilize developmental, molecular and cell biological approaches including imaging in living embryos. Applicants with a Ph.D., M.D. or equivalent and a strong background in Developmental Biology, Embryology, Cell Biology, Molecular Biology and/or Stem Cell Biology are highly encouraged to apply. Salary and benefits are commensurate with relevant experience. Review of applications will continue until the position has been filled.

 

Please send your CV, cover letter indicating current and future research interests, and the name/email address of three references to:

 

Rachel K. Miller, Ph.D. 

Department of Pediatrics 

The University of Texas Medical School at Houston 

Email: Rachel.K.Miller@uth.tmc.edu

https://med.uth.edu/pediatrics/miller-lab/

The Duke University School of Medicine Zebrafish Core Facilities (Z-Core) is a shared resource that offers aquaculture services to ten biomedical research labs using fish as a model organisms.  Having gone through several expansions since its inception in 2003, the Duke Z-Core now houses over 12,000 tanks. The facility utilizes seven large multi-rack recirculating aquaculture systems (RAS), a flow through system, and many small RAS units to house thousands of transgenic and mutant zebrafish and stickleback strains.  The facility staff provides fish husbandry and RAS maintenance in support of studies at the forefront of tissue regeneration, microbiota interaction, morphogenesis, cellular disease, immunology, and vertebrate evolution research.  The Z-Core’s growth and use of advanced aquaculture equipment creates a unique and growth-oriented environment for professionals looking to advance in aquaculture and aquatic science disciplines.

 

The Z-Core has an opening for an Aquaculture Manager (Lab Research Analyst 1) to be part of a dedicated husbandry staff. Candidates should have at minimum a bachelor’s degree in a scientific discipline, with a master’s degree preferred.  Ideal candidates will have at least 2-3 years of experience working with zebrafish or in a biomedical aquaculture facility.  An advanced understanding of aquaculture, husbandry, and water chemistry is expected.

 

Work responsibilities include:

1.)  Provide support to Shared Resource Manager and researchers in the daily facility functions. 

2.)  Supervise and train technicians and student helpers on required duties.

3.)  Respond to emergency situations (this may include responding at night or on weekends).

4.)  Prepare stock solutions and track inventory necessary for research procedures and fish care.

5.)  Manage quarantine room to ensure biosecurity measures are being exercised and fish strains are transitioned properly into general holding.

6.)  Coordinate logistics of importing and exporting fish shipments.

7.)  Assist in developing or revising standard operating procedures.

8.)  Orient new facility users.  

9.)  Feed fish, maintain cultures, and clean tanks as needed.

10.)  Maintain facility cleanliness in tandem with the facility staff.

11.)  Handle requirements of quarterly sanitation testing.

12.)  Record, review, and/or adjust daily water parameters.

13.)  Perform daily health checks and work with veterinarians and staff to resolve any health abnormalities.

14.)  Ensure facility equipment is maintained, calibrated, and operating correctly.

15.)  Monitor embryo care, survival, and transfer of tanks on or off of nursery racks.

16.)  Maintain common wild-type, transgenic, and mutant fish stocks.

17.)  Participate and occasionally lead in bi-weekly staff meetings.

18.)  Contribute ideas on facility improvements regarding equipment, procedures, and techniques.

19.)  Interact and consult with facility users to ensure research goals are being met by the most humane and ethical methods in accordance with approved protocols.

 

Other duties may be required as necessary.   Training will be provided as needed.  Occasional weekend and holiday work is required.

 

Working conditions:

The Aquaculture Manager will be on his/her feet a majority of the time and will periodically need to lift items up to 50lbs.  Work will be performed in or around wet conditions that require extra caution.  Work may result in occasional exposure to malodorous smells, chemicals, and fish and bacterial waste. 

 

Qualifications:

Candidates should have at minimum a bachelor’s degree in a scientific discipline related to aquatics or animal care, with a master’s degree preferred.  Two to three years of work experience contributing to an understanding of aquaculture equipment and maintenance, fish health and husbandry, and water chemistry is expected. 

 

Salary:  DOE/DOQ

 

If interested please send cover letter and resume to Jim Burris, Shared Resource Manager, at jim.burris@duke.edu.