Do you want to join a fast growing biotech company and support our Zebrafish genome editing technologies?
NemaMetrix Inc. is a fast paced, biotechnology company based in Eugene, Oregon. Our mission is to enable scientists and researchers around the world to better understand human health by offering affordable and accessible research platforms. We specialize in model-organism bioengineering and cutting edge CRISPR techniques to develop modified C. elegans and Zebrafish that act as human proxies for disease research and drug discovery and help our customers reveal new insights into the true nature of genetic mutations.
As a member of the zebrafish team, the ideal candidate will join a dynamic group of R&D scientists and contribute to both internal and external client projects and provide technical support to our rapidly growing Zebrafish division.
Responsibilities
Zebrafish husbandry including breeding, fin clip genotyping and screening/maintaining transgenic and mutant zebrafish lines
Microinjections of zebrafish embryos
Molecular Biology Techniques including PCR, Restriction Enzyme digestion, and gel electrophoresis
Preparation of solutions, buffers or similar, as necessary to complete the above activities
Maintain appropriate record keeping and related documentation of laboratory work
Other assignments as needed
In addition, the incumbent will be required to:
Effectively communicate with colleagues
Collaborate within a team environment
Complete tasks with a high level of accuracy and timeliness
Requirements
The ideal candidate possesses the following qualifications and attributes:
BSc in Biology or related field preferred
Preference given to candidates with prior zebrafish experience
12 months post education laboratory experience preferred
Ability to work both independently and in a team-based environment
Strong written and interpersonal communication skills
Well-organized and detail oriented
Ability to multitask, prioritize, work autonomously and manage time effectively
Familiarity with cloud-based applications such as Google Docs, Benchling, and Asana
Benefits:
Competitive salary
Health and Dental coverage
Potential Stock Option incentives
Parking and public transit assistance
Flexible work schedule
To become part of our team, please advance a CV/resume and cover letter to careers@nemametrix.com. Please include “Zebrafish Technician” in the subject line and opening of your cover letter.
All applicants must be legally authorized to work within the United States, as we are unable to provide any type of sponsorship for this position.
NemaMetrix is an equal opportunity employer.
NemaMetrix recruits, employs, trains, compensates and promotes regardless of race, religion, color, national origin, sex, disability, age, veteran status, and other protected status as required by applicable law.
At NemaMetrix, we have a clear vision: to be the place where a diverse mix of talented people want to come, to stay and do their best work. We pride ourselves on bringing amazing breakthrough scientific products to our customers around the world, and we know our company runs on the hard work and dedication of our passionate and creative employees.
NemaMetrix’s dedication to promoting diversity, multiculturalism, and inclusion is clearly reflected in all of our content and across all of our efforts. Diversity is more than a commitment at NemaMetrix—it is the foundation of what we do. We are fully focused on equality and believe deeply in diversity of race, gender, sexual orientation, religion, ethnicity, national origin and all the other fascinating characteristics that make us different.
Company Name: University of Massachusetts Medical School
Job Title: Research Lab Tech II
Start Date: 11/27/2018
Stop Date: 03/28/2019
GENERAL SUMMARY OF POSITION:
Under the direct supervision of a Principal Investigator or designee, the Research Lab Tech II performs and interprets routine and highly complex experiments.
MAJOR RESPONSIBILITIES:
- Performs a variety of laboratory experiments using both routine and special techniques and methods
- May assist in the training of others
- Provides input to improve current procedures, protocols, and/or methods
- Writes standard operating procedures, schedules work, orders supplies, and maintains an adequate inventory of supplies, reagents, etc.
- Prepares laboratory reagents, buffers, and media
- Records results, organizes data and performs computations
- Performs duties independently; requiring action and judgment to solve commonly encountered problems
- Complies with all safety and infection control standards appropriate to this position
- Perform other duties as required.
REQUIRED QUALIFICATIONS:
- Bachelor's degree in a Biological Science or equivalent experience
- 1 year of related experience
- Knowledge of Microsoft Office products
- Ability to perform duties independently
- Judgment and action skills required to solve commonly encountered problems
PREFERRED QUALIFICATIONS:
Experience Required:
- Experience with zebrafish husbandry.
- Experience with zebrafish embryo microinjection
Experience Desired (not required):
- Basic molecular biology techniques (PCR, Restriction Enzyme mediated cloning, Gel electrophoresis, Qiagen DNA miniprep).
- Bacteria Strain preparation and handling (competent cell preparation and agar plate preparation).
SUPERVISION RECEIVED:
Under the direct supervision of a Principal Investigator or designee
SUPERVISION EXERCISED:
None
ENVIRONMENTAL WORKING CONDITIONS:
Wet chemistry laboratory, and may require appropriate contact with biohazards, radionuclides, toxins, animals, and human specimens.
Apply Here: http://www.Click2apply.net/jqrt3rjtg8qms775
PI105817739
Postdoc position, #2018—31060, at UMass Medical School in the Dept of Molecular, Cell and Cancer Biology
Postdoctoral Position in Vascular Development
A postdoctoral position is available in the Lawson Lab at the University of Massachusetts Medical School. The Lawson Lab takes advantage of the zebrafish as a model system to study multiple aspects of vascular and lymphatic development. Current areas of interests include: origins of endothelial and mural cell heterogeneity, developing zebrafish as a platform to identify small molecule therapeutics, and optimization of genome editing approaches for targeted gene knock-in in zebrafish. Our efforts are supported by a recently awarded R35 grant from NHLBI that provides generous support for the next 7 years. An overview of our past and ongoing research can be found at our website (http://lawsonlab.umassmed.edu).
REQUIRED QUALIFICATIONS:
PhD degree required. Applicants must be highly motivated and technically accomplished as documented by exceptional publication record. Candidates must have demonstrated experience in molecular biology. Experience with developmental model systems is preferred.
TO APPLY:
Please email CV and reprints of relevant publications to the contact information below. Applicants accepted for initial consideration will be subsequently contacted for letters of reference.
Nathan D. Lawson, PhD
Professor
Department of Molecular, Cell, and Cancer Biology
University of Massachusetts Medical School
Lazare Research Building, Room 617
364 Plantation Street
Worcester, MA 01605
e-mail: nathan.lawson@umassmed.edu
UMass Medical School is committed to being an equal opportunity and affirmative action employer and recognizes the power of a diverse community. We encourage applications from protected veterans, individuals with disabilities and those with varied experiences, perspectives and backgrounds to consider UMass Medical School as their employer of choice.
The Group of “Neuro and Vascular Development” (Prof. Dr. Amparo Acker-Palmer) at the Goethe University Frankfurt am Main invites applications for a FOR-funded
PhD Position in Zebrafish Genetics and Imaging
(E13 TV-G-U, 65 %)
This position is initially available starting from 01.01.2019 until December 31st, 2021.
The wage classification conforms to the job characteristics of the collective agreement applicable to the Goethe-University.
We are looking for a highly motivated scientist, with a master in natural sciences and ideally with a cell- and developmental biology background, to join our team.
The Goethe University Frankfurt, positioned among the top international research universities, offers a wide variety of academic programmes, a diverse group of research institutes, and a focus on interdisciplinary approaches to solving complex problems. Today, the Goethe University is the only university in Germany which enjoys significant public funding alongside administrative autonomy and the ability to create a private endowment. Situated in Germany’s most cosmopolitan and international city, the university attracts a diverse body of students and researchers from around the world and is with about 46.000 students and 4.600 employees the biggest university in Hessen.
Our group is part of the Buchmann Institute for Molecular Life Sciences and of the Institute of Biosciences, located at the Campus Riedberg. The Institute is composed of nineteen scientific groups from various disciplines including biology, physics, chemistry, and medicine. We are dedicated to basic research with biomedical relevance, to train young scientists, and to develop frontier technologies in life sciences.
The Acker-Palmer Group investigates the molecular signals that coordinate the development and plasticity of the neurovascular interface. We are using a combination of state-of-the-art inducible and cell type-specific genetics, both in mouse and zebrafish, together with confocal and two-photon light microscopy, tissue explants, cell culture, proteomics and biochemical assays (see Nature Communications 6: 6576, Developmental Cell 28: 381-393, Neuron 84: 370-85, Nature 472: 356-60/478:274, Nature 465: 487–491, Nature Neuroscience 11: 1035-1043, Nature Neuroscience 10: 301-310, Science Vol. 361, Issue 6404). From our insights into basic molecular mechanisms, we hope to develop novel therapeutic strategies for the treatment of neurological and psychiatric diseases. Please visit our website www.neuro-vessel-frankfurt.de for more details on our research.
Your task:
You will work in a dynamic international team to study the neurovascular crosstalk in the zebrafish using genetics and state-of-the-art imaging.
Your qualifications:
We look for a candidate with a master in natural science and a solid experience in lab work and with the model organism zebrafish. Experience in cell biology, molecular biology methods and confocal imaging is required. Candidates with solid knowledge in microinjection, TALEN/CRISPR mutagenesis, in vivo imaging and image analysis (ImageJ, Metamorph, Imaris) will be preferred. Excellent knowledge of the English language (spoken and written) is required.
We offer:
We offer excellent facilities to conduct cutting-edge research in an international team atmosphere. You will collaborate with internal and external partners. You will participate in tailored interdisciplinary training and a framework of common scientific activities. The laboratory is affiliated with the Max Planck Institute of Brain Research in Frankfurt through the Max Planck Fellow program.
Application:
Please submit your application, containing a cover letter that states your experience and motivation and your curriculum vitae until December 11th, 2018 at the latest to: bataille@bio.uni-frankfurt.de (c/o Prof. Dr. Acker-Palmer, Fachbereich Biowissenschaften, Johann Wolfgang Goethe-University Frankfurt am Main).
The Department of Molecular Pathology/Cancer Center at Massachusetts General Hospital is seeking two research fellows to work in the laboratory of Dr. David Langenau. As part of the dynamic network of researchers in both the Department of Pathology and the Cancer Center at Massachusetts General Hospital, these individuals will be integrated into both the medical and research community at Harvard Medical School.
Research will focus on using zebrafish cancer models, mouse xenografts, and human cell lines to better understand how pediatric muscle tumors and leukemia relapse following conventional chemotherapeutic intervention. Projects will center on using cutting-edge live cell imaging to visualize cancer development in fluorescent-labeled zebrafish. Work will also involve use of human cell lines, biochemistry, molecular biology, and mouse xenografts. Dr. Langenau’s research interests are best summarized in the following manuscripts (Hayes et al., Cell Stem Cell 2018; Lobbardi et al., Cancer Discovery 2017; Tenente eLife 2017; Blackburn et al., Cancer Cell 2014; Ignatius et al., Cancer Cell 2012).
Please send a CV/resume and 2 references of contact to Dr. David Langenau (dlangenau@mgh.harvard.edu). Please title your email “Fellowship Application”. Salary will be based on years of experience and will be competitive with pay scales at all Harvard Affiliated Hospitals.
David M. Langenau, PhD
Associate Chief of Research and
Director of Molecular Pathology, Massachusetts General Hospital
Associate Professor of Pathology, Harvard Medical School
149 13th Street, office #6012
Charlestown, Massachusetts 02129
www.hsci.harvard.edu/people/david-m-langenau-phd
The Zebrafish Neurodevelopment Lab (PI: Julia Ganz) at Michigan State University is recruiting highly motivated PhD students interested in working on the development and regeneration of the nervous system.
In the Ganz Lab (https://www.ganzlab.org/), we are interested in understanding how a complex nervous system is generated from stem/progenitor cells and how regeneration of a nervous system is regulated using zebrafish as our model system. In our lab, we focus on development of the enteric nervous system that innervates the gut and controls important gut function, such as gut motility. We use a variety of molecular (i.e. CRISPR/Cas9 genome editing, single cell RNA-seq, in situ hybridization, immunohistochemistry) and imaging techniques (confocal and spinning disk microscope) to identify the signals that stem/progenitor cells require to generate different types of neurons and glia cells in the enteric nervous system. We are also evaluating if the enteric nervous system regenerates and if it does, how that process is regulated.
Possible projects fall within the following research areas in the lab:
How is the generation of neurons, glia cells and different neuronal subtypes regulated in the enteric nervous system?
We are testing candidate genes identified from RNA-seq screens to identify genes and signaling pathways that play a role in regulating neuronal differentiation or in neuronal subtype specification. We then dissect the molecular and cellular basis of their effect on neuronal or glial differentiation or neuronal subtype specification.
How is regeneration in the enteric nervous system regulated?
Using a genetic cell ablation system with spatio-temporal control, we will determine the cellular
responses after targeted neuronal ablation and the regenerative ability of the ENS. Using
molecular and imaging techniques, we will determine how the regenerative process is regulated
– is there a regenerative program or does the regenerative process recapitulate development?
Gene family evolution (shared project with the Braasch Lab at MSU)
In collaboration with the Braasch Lab we tackle the question: what is the role of gen(om)e duplications and gene losses in generating phenotypic diversity? We focus on gene families that play a role in neural crest and nervous system development/function combining developmental analyses with evolutionary approaches.
Our group is part of the Department of Integrative Biology (IBIO) (https://integrativebiology.natsci.msu.edu/), Neuroscience Program (https://neuroscience.natsci.msu.edu/), Reproductive and Developmental Sciences Program (http://rdsp.canr.msu.edu/), and the Genetics Graduate Program (https://www.genetics.msu.edu/) that is part of the BMS program (https://biomolecular.natsci.msu.edu/) at Michigan State University.
MSU IBIO has a strong research commitment to vertebrate biology with a highly collaborative community of groups working on the enteric nervous system, gastrointestinal research, development, stem cells, neuroscience, genomics, and fish evolution, allowing for vibrant exchange among fields, methods and model systems.
Qualifications: Applicants should have training in biology, neurobiology, genetics, molecular biology, cell biology, zoology or related fields. Suitable candidates should be enthusiastic about developmental biology and neurobiology. Previous research experience in a relevant area is desired.
PhD candidates should email Julia Ganz in advance of the MSU Graduate Program application deadlines on December 1, 2018.
Please include the following in your email:
1. Description of your research interests and how they align with research interests of my lab
2. Curriculum Vitae
3. Names and email contacts of 2-3 references
We are looking forward to your application!
Dr. Julia Ganz
Assistant Professor
Department of Integrative Biology
College of Natural Science
Michigan State University
ganz@msu.edu; phone: +1 (517) 432-3484
www.ganzlab.org
https://www.researchgate.net/profile/Julia_Ganz
Twitter: @brainyfishguts
PhD Positions in Fish Evolutionary Developmental Genomics
The Fish Evo Devo Geno Lab at Michigan State University (PI: Ingo Braasch) is recruiting highly motivated PhD students interested in working on the genomic basis of vertebrate evolution and development to start in Summer/Fall 2019.
The Braasch Lab focuses on genomic and developmental changes that contribute to major transitions during the course of vertebrate evolution and studies evolutionary novelties at the levels of genome structure, gene family evolution, and gene regulation. We combine sequencing and comparative analyses of fish genomes with analyses of molecular evolution and functional genetic and developmental approaches (CRISPR genome editing, transgenics, gene expression analyses, epigenomic profiling) in a variety of model species (zebrafish, spotted gar, medaka, and others).
Graduate projects fall within the following broader research areas of the group:
1. Evolutionary genomic analyses of zebrafish and other biomedical fish models
Combining genomic sequence comparisons, gene expression analyses and epigenomic profiling, we aim to improve connectivity of teleost biomedical fish models such as zebrafish, medaka, killifishes, etc. to human biology and disease. We use ‘bridge species’ such as spotted gar to translate biomedical research from zebrafish to human and vice versa. This work is support by the NIH.
2. Genomic and morphological evolution of fishes
How do morphological differences among fish and other vertebrate lineages arise from diversification of gene repertoires? What is the role of gen(om)e duplications and gene losses in generating phenotypic diversity? How do changes in gene regulation contribute to evolutionary novelties and key innovations? We study a number of gene families that are of particular importance for the evolution of the vertebrate body plan, e. g. genes involved in development of the vertebrate-specific neural crest cells.
3. Conquest of land and ‘fish-out-of-water’
We are studying genomic changes and their functional consequences leading to the evolution of tetrapods from fishes and other ‘fish-out-of-water’ scenarios, including the evolutionary loss of genes at the water-to-land transition and the gene regulatory basis of hatching.
For additional information on our research, see also Braasch et al. 2016, Nature Genetics (doi:10.1038/ng.3526) and Braasch et al. 2015, JEZB (doi:10.1002/jez.b.22589).
The Fish Evo Devo Geno Lab is part of the Department of Integrative Biology (IBIO), the Ecology, Evolutionary, and Behavior Program (EEBB), the Genetics Graduate Program at Michigan State University, and member of the NSF BEACON Center for the Study of Evolution in Action.
MSU IBIO has a strong research commitment to vertebrate biology with a highly collaborative community of groups working on vertebrate and fish evolution, genomics, development, population genetics, neuroscience, behavior, ecology, and conservation, allowing for vibrant exchange among fields, methods and model systems.
Qualifications:
Applicants should hold a bachelor’s degree in biology, genetics, genomics, molecular biology, bioinformatics, developmental biology, zoology or related fields. Suitable candidates should be enthusiastic about working in an interdisciplinary manner and have a passion for fish/vertebrate biology and evolution. Previous research experience in a relevant area is desired, but not required.
Admission:
Students will be admitted through the MSU IBIO Graduate Program (https://integrativebiology.natsci.msu.edu/graduate-program/) and the MSU Ecology, Evolutionary Biology, and Behavior Program (https://eebb.natsci.msu.edu/). Another possible route of admission is through the MSU Genetics Graduate Program within the MSU BioMolecular Science Gateway (https://biomolecular.natsci.msu.edu/applicants/how-to-apply/).
Application deadline for the MSU IBIO and Genetics Graduate Programs are December 1, 2019.
Funding:
Financial support is provided through research and teaching assistantships and the PI’s external funding. Competitive applicants will be eligible for university fellowships and supported in applying for graduate fellowships from NSF, NIH, and other agencies.
Interested candidates should email Ingo Braasch (braasch@msu.edu) in advance of the application deadlines on December 1.
Please include the following in your email:
1. Brief description of your research interests and how they align with pursuing a PhD in vertebrate Evo-Devo and genomics
2. Curriculum Vitae
3. Names and email contacts of 2-3 references
We are looking forward to your application!
Ingo Braasch
Assistant Professor
Department of Integrative Biology
College of Natural Science
Michigan State University
braasch@msu.edu; phone: +1 (517) 432-3484
Twitter: @fishevodevogeno
The University of Luxembourg has the following vacancy in the Luxembourg Centre for Systems Biomedicine (LCSB):
Postdoctoral Researcher in Systems Biomedicine of the Epilepsies (m/f)
- Ref. 50013760 – (R-AGR-3327-12-C)
- Fixed-term contract 3 years, renewable once, competitive salary
- Full-time position (40h/week)
- Start date: 1st December 2018 or asap
Your Role
We seek a highly skilled postdoc to study the genetics of epileptogenesis in zebrafish. The project will include generation of fish lines with epilepsy specific mutations and deep phenotyping of these by light sheet microscopy and single cell RNA sequencing. The position is part of German-Luxembourgish research unit focusing on the complex genetics of generalized epilepsies.
Your Profile
- The candidate has obtained a PhD in biology, biotechnology, bioinformatics or another life science related discipline.
- The candidate has experience in molecular biology, microscopy, neuroscience and/or zebrafish.
- Programming skills and knowledge of biomedical data are a plus but not obligatory.
- Fluency in oral and written English
Further Information
Dr. Alexander Skupin
Further Information
Applications should contain the following documents:
- A detailed Curriculum vitae
- A motivation letter, including a brief description of past research experience and future interests.
- Copies of Master’s and PhD degree or equivalent certificate if not yet received
- Name and contact details of at least two referees
The University of Luxembourg is a multilingual, international research University.
The University of Luxembourg is an equal opportunity employer. All applications will be treated in the strictest confidence.
Please apply online until November 15th, 2018 using this link: http://emea3.mrted.ly/1zxuj