Post-Doctoral Positions in Zebrafish Hematopoiesis and Metabolism - Virginia Commonwealth University
Post-Doctoral Positions in Zebrafish Hematopoiesis and Metabolism
Post-doctoral positions are available for highly-motivated scientists to work on generating and characterizing zebrafish models of hematopoiesis, metabolism, and cancer. Preference will be for individuals experienced in zebrafish breeding, microinjections, microscopy, and CRISPR-based editing. The zebrafish laboratory has 600 sq ft of new aquatic facilities and 600 sq ft of lab equipped for molecular biology and advanced microscopy in a strong collaborative environment with other zebrafish investigators. Please send CV with cover letter with scientific interests and goals to: Seth Corey, MD Children’s Hospital Foundation Endowed Professorship of Pediatric Cancer Research Professor, Pediatrics, Microbiology/Immunology, and Human and Molecular Genetics, and Co-Leader, Cancer Molecular Genetics Program at the Massey Cancer Center at Virginia Commonwealth University: coreylab@mac.com
A position for a research technician to manage a zebrafish facility is available starting immediately. The facility supports research in the Sadler laboratory, part of a thriving research community at NYUAD. The research technician will manage a colony of over 2,000 zebrafish from over 40 different genetically modified lines, which form the basis for research on liver development and disease. Responsibilities include breeding, feeding and rearing a colony of healthy zebrafish, genotyping using PCR, routine maintenance and occasional repair of aquaculture equipment and fish housing system, importing and exporting fish for collaborations, administrative responsibilities required for running the facility including maintaining service logs and colony inventories, training and oversight of technical staff and training new lab members on fish protocols. Candidates interested in research will have the opportunity to pursue independent projects related to the research interests of the labs. A high degree of organization, attention to detail, initiative and the ability to maintain a strict routine is absolutely required. In addition to the excellent benefits provided to employees of NYUAD and advantages of living in Abu Dhabi, this position has competitive remuneration and outstanding benefits.
Requirements:
- B.S. (or equivalent) in Biology, Fisheries or related fields.
- Experience with zebrafish.
- Experience with and understanding of molecular biology techniques including PCR, and genetic principles.
- Demonstrated ability to be highly organized and have an extraordinary dedication to excellence: This facility carries fish for on average 10 researchers, and responsibilities include tracking, maintaining an accurate inventory and sufficient numbers of all lines, with an extreme attention to detail.
- Outstanding ability to work as part of a team and a positive, friendly attitude and to communicate clearly and effectively.
- The ability to work weekend, holiday and evening shifts and to live in close proximity to the NYUAD campus to be on call for emergencies.
Strongly desired:
- Comfortable troubleshooting electrical/mechanical issues.
- Excellent grades in in a university level course in genetics and molecular biology.
- Aquaculture or animal management experience .
- Experience with microscopy.
- Ability to lift 15 kg.
TO APPLY:
Please email as a single PDF your cover letter stating your interest in and qualifications for this position and a CV includes and starts with the following information:
1. Name and current address (city and country)
3. Degree, date awarded and degree granting institution.
4. Date of availability.
5. Name, e-mail and phone number of three references who can comment on your abilities.
CONTACT:
Kirsten Sadler Edepli at Kirsten.Edepli@nyu.edu
http://nyuad.nyu.edu/en/academics/faculty/kirsten-sadler-edepli.html
About SickKids
Dedicated exclusively to children and their families, The Hospital for Sick Children (SickKids) is one of the largest and most respected paediatric healthcare centres in the world. As innovators in child health, we lead and partner to improve the health of children through the integration of healthcare, leading-edge research and education. Our reputation would not have been built – nor could it be maintained – without the skills, knowledge and experience of the extraordinary people who come to work here every day. SickKids is committed to ongoing learning and development, and features a caring and supportive work environment that combines exceptionally high standards of practice.
When you join SickKids, you become part of our community. We share a commitment and determination to fulfill our vision of Healthier Children. A Better World. Don’t miss out on the opportunity to work alongside the world’s best in paediatric healthcare.
Position Description
We require an enthusiastic individual with genetics and molecular biology experience. You will be a key team member in the Zebrafish Genetics and Disease Models Core Facility (Zebrafish Core) in the Peter Gilgan Centre for Research and Learning. The Zebrafish Core works closely with clinicians and scientists to create disease models and facilitate both phenotype analysis and drug discovery.
Here’s What You’ll Do:
In close collaboration with the facility manager, you will help establish the Zebrafish Core as an essential part of the SickKids research environment. This position includes, but is not limited to: ordering supplies, making reagents, managing equipment, generation of zebrafish mutants, designing PCR primers, sequence analysis, molecular cloning, tracking client orders, maintaining zebrafish lines, breeding zebrafish and microinjection on client request, occasional feeding of zebrafish, performing small molecule suppressor screens, writing project update reports, and learning new techniques as required.
Here’s What You’ll Need:
Candidates must have at minimum an MSc, or a BSc with at least 1 year experience in both molecular biology research and zebrafish husbandry, breeding and microinjection techniques. Individuals must be highly organized, be able to multi-task and handle multiple projects at the same time, have attention to detail, and be enthusiastic and inquisitive. Experience with the following techniques is preferred: PCR, primer design, CRISPR/Cas9 mutagenesis, agarose gel electrophoresis, molecular cloning, sequence analysis, brightfield and confocal imaging, and genetics. Candidates must have the ability to work independently while effectively communicating and coordinating with the facility manager. Excellent communication skills (written and spoken) in English required. Must be proficient in Microsoft Office applications.
Employment Type: Temporary Contract, 1 Year
Our Commitment to Diversity
SickKids is strongly committed to diversity within its community and welcomes applications from visible minority group members, women, Aboriginal persons, persons with disabilities, members of sexual minority groups and others who may contribute to diversity within our organization.
SickKids is also committed to providing services in both official languages and our preference is to hire employees who are fluent in both English and French whenever possible.
Thank you for your interest in joining SickKids. Only those applicants selected for an interview will be contacted. In accordance with our Hospital policy, employment at SickKids is conditional upon the Hospital’s receipt of all necessary documentation.
How to Apply
Tip: Combine your cover letter and resume into ONE document as you cannot upload multiple documents as part of your application.
Apply here:
Uppsala University is a comprehensive research-intensive university with a strong international standing. Our mission is to pursue top-quality research and education and to interact constructively with society. Our most important assets are all the individuals whose curiosity and dedication make Uppsala University one of Sweden’s most exciting workplaces. Uppsala University has 42,000 students, 7,000 employees and a turnover of SEK 6.7 billion.
The Department of Immunology, Genetics and Pathology at Uppsala University (www.igp.uu.se) has a broad research profile with strong research groups focused on cancer, autoimmune and genetic diseases. A fundamental idea at the department is to stimulate translational research and thereby closer interactions between medical research and health care. Research is presently conducted in the following areas: medical and clinical genetics, clinical immunology, pathology, neuro-oncology, vascular biology, radiation science and molecular tools. Department activities are also integrated with the units for Oncology, Clinical Genetics, Clinical Immunology, Clinical Pathology, and Hospital Physics at Akademiska sjukhuset, Uppsala. The department has teaching assignments in several education programmes, including Master Programmes, at the Faculty of Medicine, and in a number of educations at the Disciplinary Domain of Science and Technology. The department has a yearly turnover of around SEK 375 million, out of which more than half is made up of external funding. The staff amounts to approximately 340 employees, out of which 120 are PhD-students, and there are in total more than 600 affiliated people.
Project description
Genome-wide association studies (GWAS) have identified >100 genetic loci that are robustly associated with the risk of diabetes. With few exceptions, the causal genes in these loci are currently unknown. Before results from GWAS can be translated into the clinic - for example as novel biomarkers or drug targets - we need to identify and characterize the causal genes in these loci. Recent developments in CRISPR-Cas9-based mutagenesis, high-throughput imaging, and image-based analyses have highlighted the zebrafish as a promising model system for systematic, large-scale genetic screens.
The overall aim of the proposed project is to identify and characterize causal genes in GWAS-identified loci for diabetes using zebrafish model systems.
Work description:
The position is funded by an international collaborative grant that aims to characterize diabetes candidate genes using human beta-cells (Oxford), drosophila (Stanford) and zebrafish (Uppsala). The zebrafish arm of the project includes: 1) designing and testing of CRISPR-Cas9 gRNAs for efficiency; 2) generating and maintaining mutant lines using a multiplex approach, i.e. studying the effect of multiple genes simultaneously; 3) imaging diabetes-related traits using an automated positioning system and fluorescence microscope; 4) preparing samples for enzymatic assessment of whole-body lipid and glucose levels and paired-end sequencing; 5) objective quantification of image-based data; 6) quality control and analysis of sequencing results; 7) integration of multidisciplinary data and statistical analysis; and 8) dissemination of results into manuscripts for publication in peer-reviewed journals.
Qualifications:
Applications are accepted from highly motivated candidates with a PhD in Molecular Biology, Molecular Epidemiology, or similar.
Applicants must have a documented and broad competence in basic molecular biology methodology. A successful candidate should be a highly motivated, organized, reliable team player that can also work independently and is proficient in communicating in English, both orally and in writing.
Merits:
Prior knowledge and experience in fluorescence microscopy, epidemiology, bioinformatics, programming and/or image-based analyses are a bonus.
The application should include a cover letter describing yourself, your research interests and your experience relevant to this position; a CV/resume; a verified list of course grades; a list of publications; and contact details for at least two reference persons. If available, letters of recommendation can also be included.
Salary: According to individual qualifications
Start date: as soon as possible
Form of employment: general temporary employment of two years
Extent of employment: 100%
For more information contact:
Marcel den Hoed,marcel.den_hoed@igp.uu.se ,070-4250752
You are welcome to apply no later than March 31, 2018. UFV-PA 2018/303.
Apply here:
https://www.uu.se/en/about-uu/join-us/details/?positionId=192994
Uppsala University is a comprehensive research-intensive university with a strong international standing. Our mission is to pursue top-quality research and education and to interact constructively with society. Our most important assets are all the individuals whose curiosity and dedication make Uppsala University one of Sweden’s most exciting workplaces. Uppsala University has 42,000 students, 7,000 employees and a turnover of SEK 6.7 billion.
The Department of Immunology, Genetics and Pathology at Uppsala University (www.igp.uu.se) has a broad research profile with strong research groups focused on cancer, autoimmune and genetic diseases. A fundamental idea at the department is to stimulate translational research and thereby closer interactions between medical research and health care. Research is presently conducted in the following areas: medical and clinical genetics, clinical immunology, pathology, neuro-oncology, vascular biology, radiation science and molecular tools. Department activities are also integrated with the units for Oncology, Clinical Genetics, Clinical Immunology, Clinical Pathology, and Hospital Physics at Akademiska sjukhuset, Uppsala. The department has teaching assignments in several education programmes, including Master Programmes, at the Faculty of Medicine, and in a number of educations at the Disciplinary Domain of Science and Technology. The department has a yearly turnover of around SEK 375 million, out of which more than half is made up of external funding. The staff amounts to approximately 340 employees, out of which 120 are PhD-students, and there are in total more than 600 affiliated people.
Project description:
Genome-wide association studies (GWAS) have identified >100 genetic loci that are robustly associated with the risk of diabetes. With few exceptions, the causal genes in these loci are currently unknown. Before results from GWAS can be translated into the clinic - for example as novel biomarkers or drug targets - we need to identify and characterize the causal genes in these loci. Recent developments in CRISPR-Cas9-based mutagenesis, high-throughput imaging, and image-based analyses have highlighted the zebrafish as a promising model system for systematic, large-scale genetic screens.
The overall aim of the proposed project is to identify and characterize causal genes in GWAS-identified loci for diabetes using zebrafish model systems.
Work description:
The position is funded by an international collaborative grant that aims to characterize diabetes candidate genes using human beta-cells (Oxford), drosophila (Stanford) and zebrafish (Uppsala). The zebrafish arm of the project includes: 1) designing and testing of CRISPR-Cas9 gRNAs for efficiency; 2) generating and maintaining mutant lines using a multiplex approach, i.e. studying the effect of multiple genes simultaneously; 3) imaging diabetes-related traits using an automated positioning system and fluorescence microscope; 4) preparing samples for enzymatic assessment of whole-body lipid and glucose levels and paired-end sequencing; 5) objective quantification of image-based data; 6) quality control and analysis of sequencing results; 7) integration of multidisciplinary data and statistical analysis; and 8) dissemination of results into manuscripts for publication in peer-reviewed journals.
Qualifications:
Applications are accepted from highly motivated candidates with a PhD in Molecular Biology, Molecular Epidemiology, or similar. It must have been completed within three years of the application deadline. If you received your PhD earlier but special circumstances apply (i.e. prolonged periods of illness, parental leave, military service, union duties and others of similar character) then you may also be eligible to apply.
Applicants must have a documented and broad competence in basic molecular biology methodology. A successful candidate should be a highly motivated, organized, reliable team player that can also work independently and is proficient in communicating in English, both orally and in writing. Prior knowledge and experience in fluorescence microscopy, epidemiology, bioinformatics, programming and/or image-based analyses are a bonus.
The application should include a cover letter describing yourself, your research interests and your experience relevant to this position; a CV/resume; a verified list of course grades; a list of publications; and contact details for at least two reference persons. If available, letters of recommendation can also be included.
Salary: According to individual qualifications
Start date: as soon as possible
Form of employment: general temporary employment of two years
Extent of employment: 100%
For more information contact:
Marcel den Hoed, marcel.den_hoed@igp.uu.se ,070-4250752
You are welcome to apply no later than March 31 2018. UFV-PA 2018/302.
Apply here:
https://www.uu.se/en/about-uu/join-us/details/?positionId=192981
The Center Human Disease Modeling, a part of Duke University Medical Center, occupies a prominent place of national and international leadership in the fight against inherited diseases. Our mission is to develop and implement biological assays to understand genetic variation discovered in patients by bridging genetics, genomics, cell biology, and clinical investigation. We focus our efforts in the following four major areas:
1.Acceleration of gene discovery.
2.Synthesis with clinical investigation with basic experimental biology to facilitate molecular diagnosis and, when possible, treatment.
3.Generation of experimentally tractable models for entry points into mechanism of disease pathogenesis.
4.Generation of in vitro and in vivo assays that can model human disorders and are suitable for medium- and high throughout drug screening.
We are recruiting a Laboratory Research Analyst that will be responsible for providing aquaculture support in a zebrafish facility and a variety of technical duties in a research laboratory. This individual will be the point person in one of four Duke Z-Core zebrafish facilities and will work with husbandry staff to monitor embryo care, survival, and transition of tanks on/off nursery racks, maintain facility wild-type, transgenic and mutant stocks, and assist in managing new zebrafish lines. Additionally, the candidate will lead projects that employee the use of zebrafish as a model system utilizing techniques such as molecular cloning, DNA/RNA extraction, DNA sequencing, gene expression, zebrafish embryo manipulation, immunostaining and microscopy. This individual will document, analyze, interpret and summarize experimental results, as well as orient, supervise, and train staff in the operations of the zebrafish facility. The ideal candidate will have a Bachelor’s degree in biology, zoology, biochemistry, or related field with a minimum of two to four years of relevant laboratory experience. Master's degree may substitute for experience. Candidates should apply to requisition #401388425 on the Duke employment website at www.hr.duke.edu and submit your CV (PDF, Word or video format), brief statement of interest, and contact information for three references to:
CHDM Human Resources chdmjobs@duke.edu Please reference requisition number in the subject line.
Duke University is an Affirmative Action/Equal Opportunity Employer committed to providing employee opportunity without regard to an individual’s age, color, disability, genetic information, gender, gender identity, gender expression, national origin, race, religion, sex, sexual orientation, or veteran status.
Postdoctoral positions are available in the Waxman lab at Cincinnati Children’s Hospital Medical Center. The Waxman Lab uses zebrafish as a model to elucidate mechanisms underlying vertebrate heart development, with a focus on determinants of cardiac chamber size. Applicants should be motived, creative individuals that have recently obtained a PhD or will obtain a PhD in the near future. Experience using zebrafish or studying cardiovascular development is not a requirement. Interested applicants should email a PDF containing their CV, a statement describing their past research, and the names of at least three references to Joshua Waxman (joshua.waxman@cchmc.org).
Joshua Waxman, PhD
Associate Professor
Molecular Cardiovascular Biology Division
Cincinnati Children’s Hospital Medical Center
www.cincinnatichildrens.org/research/divisions/m/mcb/labs/waxman/default/
Mechanosensory basis of spatial navigation in zebrafish
PhD position with Dr. Hernán López-Schier
Location: Helmholtz Zentrum & LMU, Munich, Germany
Available immediately (open until position is filled)
We are looking for a motivated student to conduct a PhD project in an interdisciplinary team that uses the zebrafish to understand the anatomical and functional basis of directional mechanosensation. The proposed project will study mechanical sensing during oriented swimming (rheotaxis) in larval zebrafish. Our group has access to state-of-the-art 3 dimensional behavioural tracking technology, imaging instrumentation to monitor neuronal activity, genetic tools and light- and electron-microscopic reconstructions of neuronal projections and connectivity. We are immediately interested in developing ethograms, in individuality during behaviour, as well as in identifying the flow of mechnosensory information from the peripheral receptors to the brain. Candidates should have a background, or be willing to acquire working knowledge, in computer programming and machine learning.
The student will be affiliated to, and eventually received a degree from, the LMU (ideally) or the TUM.
The working language of the Centre is English. The Helmholtz Zentrum campus is located on the north side of Munich, with a short bicycle or bus ride from the city centre.
For further information regarding this position please contact Hernán López-Schier at <hernan.lopez-schier@helmholtz-muenchen.de>
To apply please send an email including a statement of interest, a complete curriculum, and the name and contacts of 2 or 3 academic references.