Langenau Laboratory Research Fellowship in Pediatric Cancer and Zebrafish Models
The Langenau Laboratory at Harvard Medical School and the Massachusetts General Hospital, Boston are recruiting research fellows to study mechanisms of progression and relapse in T and B cell leukemia and rhabdomyosarcoma – a tumor of muscle. Research will focus on using the zebrafish genetic model, biochemistry, and cross-species bioinformatics approaches to identify novel pathways that drive progression and relapse. Following discoveries made in the zebrafish model, work will continue in characterizing discoveries in human cell culture, primary patient samples, and mouse xenograft studies.
Dr. Langenau’s research group has become a pioneer in the field and made seminal discoveries using the zebrafish model. The laboratories interests are best summarized in the following manuscripts (Lobbardi et al., Cancer Discovery, 2017; Tang et al., JEM 2017; Moore et al., JEM, 2016; Tang et al., Nature Communications, 2016; Blackburn et al., Cancer Cell 2014; Ignatius et al., Cancer Cell 2012). Additional information about the laboratory is available at langenaulab.com.
Applicants with advanced skills in in vivo microscopy, mouse xenograft transplantation, stem cell biology, muscle development, leukemia, blood development, bioinformatic analysis, and biochemistry (including ChIP seq) are highly desired. Background in zebrafish development and/or cancer is not required.
Candidates must have PhD and/or MD, have made significant scientific contributions through publication of high impact papers, and be enthusiastic about science.
Langenau Research Group
Molecular Pathology Unit
Massachusetts General Hospital
149 Thirteenth Street, Room 6133
Charlestown, MA 02129
A postdoctoral position to develop zebrafish xenograft assays for clinical diagnostics of tumor biopsies is available in the Glasgow Lab, Department of Oncology, Lombardi Comprehensive Cancer Center, at Georgetown University Medical Center in Washington D.C.
Candidates must have experience with zebrafish microinjections and/or cell transplantation, along with associated imaging techniques. Excellent communication skills and experience in clinical studies is a plus. Must be able to interface with clinical partners. This position in ideal for the applicant who is interested in applying zebrafish models to improve cancer diagnostics.
To apply, please send Cover Letter, CV and contact details of three references to Dr. Eric Glasgow (firstname.lastname@example.org).
Postdoc Position in Biology/Biotechnology Available – Department of Pediatrics I - University Children’s Hospital Heidelberg
The University Children’s Hospital invites applications for the position of a Postdoc within the Department of Pediatrics I.
The position is full-time (100%) and limited to 30 months with the option of extension. Salary is according to TV-L.
The project is embedded in a cooperation project between the University Children’s Hospital Heidelberg and BioQuant and the private companies ACQUIFER/DITABIS and Rapp OptoElectronic GmbH, which is funded by the Federal Ministry for Economic Affairs and Energy. Ultimate goal of this project is the development of a novel in vivo high-content screening platform that permits automated laser-assisted photomanipulation and photoablation of target structures in the larval zebrafish. This photoablation pipeline will be used to develop a model for acute kidney injury in zebrafish and, subsequently, to perform a high content screen for nephroprotective and regenerative substances.
You will work within a collaborative research team consisting of biologists, physicians, engineers and software developer.
To fulfil the position as Postdoc, applicants must have obtained a doctorate in biology or biotechnology. Previous experience working with zebrafish and high-content microscopy is preferred but not mandatory. Proficiency in the german language is not required.
Your duties and responsibilities
Development and validation of a laser-assisted and high-content compatible photoablation workflow for the induction of acute kidney injury in the larval zebrafish
Perform experimental work using zebrafish embryos and larvae (<5 dpf), e.g. microinjection, micromanipulation, compound treatment
Perform zebrafish husbandry tasks including handling of adults, crossings and embryo culture
Utilize automated high-content screening microscopes for chemical screening applications
Computer based analysis of large-scale image data by use of image processing tools
Immunohistochemistry of larval zebrafish, assays for cell death and proliferation, RNA in situ hybridization
Provide help and support in data analysis
Perform routine lab tasks and help with general laboratory management.
We offer to work within a highly motivated and interdisciplinary environment consisting of researchers of several scientific disciplines (biology, engineering, medicine). We expect a self-motivated and team oriented work attitude.
For applications and further details please contact
University of Heidelberg
Center for Pediatrics and Adolescent Medicine
Department of Pediatrics I
Dr. Jens H. Westhoff
Im Neuenheimer Feld 430
69120 Heidelberg, Germany
This application closes November 30th 2017.
Please find the job announcement on our Career Site (in German): Postdoc (m/w) der Biologie/Biotechnologie
A postdoctoral position to study genetic causes of intracranial aneurysms is available in the laboratory of Dr. Saulius Sumanas in the Division of Developmental Biology, at the Cincinnati Children's Hospital Medical Center.
The laboratory uses the zebrafish model to validate and study mutant variants associated with intracranial aneurysms which were previously identified in human individuals. The lab also has a long standing interest in the molecular mechanisms of vascular development. We are performing screens for novel potential regulators of vasculature formation followed by their characterization and functional studies. Candidates with a strong background in developmental and molecular biology are invited to apply. Prior experience in zebrafish is helpful but not a requirement.
To apply, please send a CV and contact details of three referees to email@example.com
Research Technician: The Mokalled lab at Washington University School of Medicine
The Mokalled laboratory is seeking applicants for a Research Technician position in the Department of Developmental Biology at Washington University School of Medicine (http://devbio.wustl.edu/faculty/faculty-members/mayssa-mokalled-ph.d). Our lab uses the zebrafish model system to study neural regeneration after spinal cord injury or disease. We welcome ambitious applicants with enthusiasm for neuroscience, regenerative biology, and zebrafish research. This position offers opportunities for co-authorships on published manuscripts.
- Assists with research studies, experiments and assays including collection of data, preparation of solutions and set-up and maintenance of equipment. Main experimental duties include molecular biology, zebrafish husbandry and microinjection.
- Performs data entry and maintains data files on research.
- Complies with established safety procedures and maintains required documentation on laboratory and specimen conditions.
- Ensures lab conditions and equipment are properly cleaned and maintained in accordance with established procedures.
- Bachelor’s degree and a minimum of 2 years of research experience.
- Experience with zebrafish husbandry or spinal cord research are encouraged.
Please send a cover letter, CV, and list of 3 or more references to firstname.lastname@example.org.
We are seeking 1-2 people to manage a zebrafish colony that serves laboratories studying central nervous system regeneration and blood clotting located in the Taubman Biological Sciences Research Building at the University of Michigan Medical School. The facility uses multiple recirculating systems to maintain fish in 4 rooms. The primary tasks in the position are animal care and husbandry, equipment and water testing, facility maintenance, record keeping and technical support as outlined below:
Animal care and husbandry of zebrafish:
• Feeding (Fish food preparation, brine shrimp hatching, and deciding feeding regime according to fish size and age). Occasional weekend feedings will be required.
• Daily monitoring of animal health and if necessary initiate appropriate actions.
• Breeding and stock maintenance. Includes screening fish for fluorescent reporter expression.
Equipment and water testing:
• Daily and weekly checks of water parameters.
• Maintenance of the water treatment system. Must be able to lift water-filled tanks, filter bags and carbon containers in range of 1-50 lbs.
• Cleaning, washing and disinfection of tanks and other equipment.
• Maintain accurate, detailed records utilizing written log sheets and computer databases tracking colony health, water quality parameters, animal breeding, and housing or support areas as required.
Responsibilities in support and communication:
• Timely communication (facility orientations/training, meetings, e-mails) with researchers about their animals and experiments.
• Assisting the researchers with different tasks (breeding, embryo care, fin clipping etc.).
• Maintain fish facility supplies.
• Maintain and update Standard Operation Procedures.
• Train and manage temporary employees.
• High school diploma or equivalent.
• Able to function independently and show initiative in keeping up-to-date on the latest developments in zebrafish husbandry.
• Strong data management, managerial, and organizational skills.
• Ability to lift/move up to 50 pounds.
• Computer skills appropriate for the position, including the ability to utilize common applications (e.g., email, Word, Excel).
• Excellent communication skills.
• Interest and experience in biological research.
• Bachelor's degree
• 2 or more years of experience working with zebrafish in a research environment.
• Able to work well with a variety of students and faculty.
• Experience with fluorescence microscopy.
To Apply: Send cover letter, CV and contact information for 2-3 references to Dan Goldman (email@example.com)
Neurodegeneration, aging, stem cells and wound healing in zebrafish
The German Center for Neurodegenerative Diseases (DZNE) investigates the causes of diseases of the nervous system and develops strategies for prevention, treatment and care. It is an institution within the Helmholtz Association of German Research Centers with nine sites across Germany (Berlin, Bonn, Dresden, Göttingen, Magdeburg, Munich, Rostock/Greifswald, Tübingen and Witten). The DZNE cooperates closely with universities, their clinics and other research facilities.
We offer an exciting international research environment at the DZNE in Munich to study the effects of zebrafish loss of function mutants in the progranulin genes in zebrafish. In close collaboration with the Laboratory of Dr. Jovica Ninkovic at the Helmholtz Zentrum Munich we will further determine the effect of the mutations on wound healing and neuronal stem cells. This project will enable us to identify molecular mechanisms of the neurodegenerative diseases FTD and NCL as well as the regenerative processes in the brain.
Employment, payment and social benefits are determined by the Publ Sector Collective Agreement of Germany (Tarifvertrag für den öffentlichen Dienst – TVöD Bund). The DZNE is an equal opportunity employer. It especially welcomes and encourages disabled individuals to apply.
We are looking for highly motivated candidates with a solid background in genetics, biochemistry and neurobiology. Experience working with zebrafish is desired but not mandatory. Fluency in spoken and written English is required.
Please send a single PDF file with a CV and motivation letter to:
Dr. Bettina Schmid
German Center for Neuro-
degenerative Diseases (DZNE)
D- 81377 München
A post-doctoral position is available at the Hoffman Lab at the Child Study Center and Department of Neuroscience, Yale University, New Haven, CT USA.
The goal of our laboratory is to understand the function of genes involved in autism spectrum disorders at the cellular, molecular, circuit, and behavioral levels using zebrafish as a model system. Our laboratory has generated zebrafish mutants in multiple autism risk genes using CRISPRs, which we are studying to understand how loss of autism risk genes alters fundamental processes of vertebrate brain development (Hoffman et al. 2016 Neuron).
The post-doctoral candidate will perform phenotypic analyses of multiple zebrafish mutants of autism risk genes using a combination of molecular, cellular, and imaging approaches.
1. Recent PhD or MD/PhD with strong experience in molecular biology and microscopy.
2. The ideal candidate will have a strong background in genetics and experience working with genetic models of human disease.
3. We are seeking a highly motivated, enthusiastic, and creative candidate with excellent interpersonal skills and a strong publication record.
4. Prior experience with in vivo functional imaging and computational skills are preferred but not required.
Candidates should send the following to Dr. Ellen Hoffman (firstname.lastname@example.org):
1. A cover letter stating a description of your accomplishments and interest in the lab’s research projects.
3. Contact information for three references.
More information about the laboratory can be found at: www.hoffmanlab.net.
PhD Positions in Fish Evolutionary Developmental Genomics at Michigan State University
The Fish Evo Devo Geno Lab (PI Ingo Braasch) at Michigan State University is recruiting highly motivated PhD students interested in working on the genomic basis of vertebrate evolution and development to start in Fall 2018.
The Braasch Lab focuses on genomic and developmental changes that contribute to major transitions during the course of vertebrate evolution and studies evolutionary novelties at the levels of genome structure, gene family evolution, and gene regulation. We combine comparative genomics with analyses of molecular evolution and functional genetic and developmental approaches using a variety of fishes as our model systems (spotted gar, zebrafish, medaka and others).
Possible projects fall within the following broader research areas of the group:
1. Genomic and morphological evolution of fishes:
How do changes in gene regulation contribute to evolutionary novelties and key innovations? How do morphological differences among fish lineages arise from diversification of gene repertoires? What is the role of gen(om)e duplications and gene losses in generating phenotypic diversity?
2. Conquest of land and ‘fish-out-of-water’:
We are studying genomic changes and their functional consequences leading to the evolution of tetrapods from fishes and other ‘fish-out-of-water’ scenarios, including the evolutionary loss of genes at the water-to-land transition and the gene regulatory basis of hatching.
3. Evolutionary analyses of biomedical fish genomes:
Combining genomic sequence comparisons, gene expression analyses and epigenomic profiling, we aim to improve connectivity of teleost biomedical fish models such as zebrafish and medaka to human biology and disease. This work is support by the NIH (http://bit.ly/2o8R8c1).
For additional information on our research, see also Braasch et al. 2016, Nature Genetics (doi:10.1038/ng.3526) and Braasch et al. 2015, JEZB (doi:10.1002/jez.b.22589).
Our group is part of the Department of Integrative Biology (IBIO; https://integrativebiology.natsci.msu.edu/), the Ecology, Evolutionary, and Behavior Program (EEBB; https://eebb.natsci.msu.edu/), and the Genetics Graduate Program (https://genetics.natsci.msu.edu/) at Michigan State.
MSU IBIO has a strong research commitment to vertebrate biology with a highly collaborative community of groups working on fish evolution, genomics, development, population genetics, neuroscience, behavior, ecology, and conservation, allowing for vibrant exchange among fields, methods and model systems.
Qualifications: Applicants should hold a bachelor’s degree in biology, genetics, molecular biology, bioinformatics, zoology or related fields. Suitable candidates should be enthusiastic about fish biology and excited about working in an interdisciplinary manner. Previous research experience in a relevant area is desired.
Admission: Students will be admitted through the MSU IBIO Graduate Program (https://integrativebiology.natsci.msu.edu/graduate-program/) and the MSU Ecology, Evolutionary Biology, and Behavior Group (https://eebb.natsci.msu.edu/). Another possible route of admission is through the MSU Genetics Graduate Program within the MSU BioMolecular Science Gateway (https://biomolecular.natsci.msu.edu/applicants/how-to-apply/).
Application deadlines for both the MSU IBIO and Genetics Graduate Programs are December 1, 2017.
Funding: Financial support is provided through research and teaching assistantships and the PI’s external funding. Competitive applicants will be eligible for university fellowships and supported in applying for graduate fellowships from NSF, NIH, and other agencies.
Interested candidates should email Ingo Braasch (email@example.com) in advance of the application deadlines on December 1.
Please include the following in your email:
1. Description of your research interests and how they align with pursuing a PhD in vertebrate Evo-Devo and genomics
2. Curriculum Vitae
3. Names and email contacts of three references
We are looking forward to your application!
Research Associate 1, MS - 44210BR
Yale University offers exciting opportunities for achievement and growth in New Haven, Connecticut. Conveniently located between Boston and New York, New Haven is the creative capital of Connecticut with cultural resources that include three major museums, a critically-acclaimed repertory theater, state-of-the-art concert hall, and world-renowned schools of Architecture, Art, Drama, and Music.
General Purpose: Function as a Lab Manager overseeing a Molecular Biology Lab using a zebrafish model system. Work on an independent research project, master generation of zebrafish mutants using CRISPR/Cas9 technique, maintain wild-type and mutant fish lines, characterize new mutant fish lines by genotypic and phenotypic analysis, supervise undergraduates and post-graduate associates in the laboratory and fish facility.
Required Education and Experience: Master’s Degree in a scientific discipline and one year experience or an equivalent combination of education and experience.
- Demonstrated knowledge and ability with zebrafish husbandry and maintenance.
- Demonstrated proficiency in molecular biology, including cloning, PCR, in vitro transcription, in situ hybridization, western blotting, and immunohistochemistry.
- Demonstrated ability in zebrafish analysis and mRNA injection.
- Demonstrated excellent interpersonal skills.
- Demonstrated strong work ethic.
- Preferred Education, Experience and Skills: Master’s Degree in the Biological Sciences, Neuroscience, Genetics or a related discipline, and one year experience or an equivalent combination of education and experience. 5 or more years of experience. Ability to lead and provide oversight in a lab setting.
Application: For more information and immediate consideration, please apply online at http://bit.ly/2txRpIY. Please be sure to reference this website when applying for this position.
We invite you to discover the excitement, diversity, rewards and excellence of a career at Yale University. One of the country's great workplaces, Yale University offers exciting opportunities for meaningful accomplishment and true growth. Our benefits package is among the best anywhere, with a wide variety of insurance choices, liberal paid time off, fantastic family and educational benefits, a variety of retirement benefits, extensive recreational facilities, and much more.
Yale University considers applicants for employment without regard to, and does not discriminate on the basis of an individual’s sex, race, color, religion, age, disability, status as a veteran, or national or ethnic origin; nor does Yale discriminate on the basis of sexual orientation or gender identity or expression.
A new position is available within a research group (Panula group) which works on basic mechanisms in neurologic and psychiatric diseases. This particular project involves production and phenotyping new zebrafish mutants generated using the CRISPR/Cas9 method.
The group manages a very well equipped zebrafish facility, including microinjection systems, confocal and 2-photon imaging systems, systems for quantitative behavioural analysis, good facilities for molecular cloning and quantitative expression analysis (e.g. quantitative PCR, MCID4 imaging system), and well-established international collaborations. The laboratories are located in the Department of Anatomy and Neuroscience Center (HiLIFE unit) on the Meilahti medical campus of the University of Helsinki. The campus houses about 450 PIs within the areas of life sciences.
Salary will be negotiated according to qualifications and the university salary system. An initial appointment will be made for one year, with possible extension. An ideal candidate has a recent PhD in relevant area, and thorough training and experience in imaging and genome modification methods, preferably within zebrafish field. Experience in programming, molecular biology, behavioural analysis, and gene expression analysis (transgenic animals, PCR, ish, immunocytochemistry, protein chemistry), high motivation and good communication skills are needed in the multidisciplinary group. Responsibilities include research on zebrafish disease models, molecular biology, zebrafish breeding and maintenance and participation in guidance of junior group members.
Send a CV, list of publications, and an application with names and contact information of 3 references to Professor Pertti Panula, Neuroscience Center, Department of Anatomy, POB 63 (Haartmaninkatu 8), 00014 University of Helsinki, by December 1, 2017 for immediate attention. Late applications may be considered for this or related positions, but attention is not guaranteed.
Puttonen HAJ, Semenova S, Sundvik M, Panula P. Storage of neural histamine and histaminergic neurotransmission is VMAT2 dependent in the zebrafish. Sci Rep. 2017 7:3060. doi: 10.1038/s41598-017-02981-w.
Chen YC, Semenova S, Rozov S, Sundvik M, Bonkowsky JL, Panula P. A novel developmental role for dopaminergic signaling to specify hypothalamic neurotransmitter identity. J Biol Chem. 2016 Aug 18. pii: jbc.M115.697466.
Sundvik, M., Chen, Y.-C., Panula, P.: Dynamic regulation of zebrafish histaminergic neuron population and behavior by presenilin 1. J. Neuroscience 33: 1589-1597, 2013.
Chen YC, Harrison PW, Kotrschal A, Kolm N, Mank JE, Panula P. Expression change in Angiopoietin-1 underlies change in relative brain size in fish. Proc Biol Sci. 2015 Jul 7;282. doi: 10.1098/rspb.2015.0872.
Panula, P., Chazot, P.D., Cowart, M., Gutzmer, R., Leurs, L., Liu, W.L.S., Stark, H., Thurmond, R., Haas, H.L.: International Union of Pharmacology. XCVIII. Histamine receptors. Pharmacol. Rev. 67: 601-655, 2015.
A full-time Research Associate/Lab Manager position is available at the Hoffman Lab, Child Study Center and Department of Neuroscience, Yale University, New Haven, CT USA
Our laboratory uses zebrafish as a model system to study the function of genes involved in autism spectrum disorders. The research associate will participate in daily management of the laboratory and contribute to research projects aimed at understanding how autism risk genes affect vertebrate brain development at the cellular and molecular levels. This position provides opportunities for co-authorship on published manuscripts.
1. Conduct research, working with the principal investigator to plan, design, and perform experiments. Experimental duties include molecular biology, immunohistochemistry, in situ hybridization, and zebrafish microinjection. Maintain organized data files of research.
2. General lab maintenance, including organizing databases, placing orders, maintaining stock solutions, and ensuring lab equipment is functioning properly. Maintain updated safety and animal regulatory protocols.
3. Assist in maintenance of zebrafish facility. Monitor water quality and fish health.
4. Breed and genotype zebrafish to maintain genetic lines. Maintain detailed record of zebrafish lines.
1. Master’s Degree in a relevant scientific discipline and one year of experience or an equivalent combination of education and experience. 5 or more years of experience is preferred.
2. Proficiency in molecular biology techniques, including PCR, cloning, western blotting, immunohistochemistry, and in situ hybridization.
3. Practical experience in zebrafish husbandry. The ideal candidate will have experience in zebrafish microinjection and phenotypic analysis.
4. Enthusiastic, highly motivated, strong work ethic, and excellent organizational and interpersonal skills. Prior experience in laboratory management is preferred.
To apply: Candidates should send the following to Dr. Ellen Hoffman (firstname.lastname@example.org):
1. A cover letter describing how your qualifications and interests match this position.
3. Contact information for three references.
More information about the laboratory and its research projects can be found at: www.hoffmanlab.net.
A POSTDOCTORAL FELLOW position is available in the laboratory of Dr. Jane Zhu in the Department of Biochemistry and Molecular Biology at the Mayo Clinic in Rochester MN (http://www.janezhulab.org/). Our interest is to understand the genetic and cellular basis as well as molecular mechanism of neuroblastoma initiation, maintenance and metastasis using zebrafish as a model system (Cancer Cell. 2017 Sep 11;32(3):310-323.e5). We will take the advantage of zebrafish model of neuroblastoma to understand the pathogenic role of new genetic findings emerging from integrative genomic studies of human neuroblastomas, to uncover the key signaling pathways that are altered in high-risk tumors, and to identify novel molecular targets for effective targeted therapies.
We are looking for creative and motivated applicants. Experiences with cell and molecular biology as well as animal model are essential. Please send a cover letter, CV and a list of three individuals as references to email@example.com
The Center for Comparative Medicine at Baylor College of Medicine is looking to hire a Lab Animal Technician III to support the zebrafish program
- Provide routine care and husbandry including feeding, water exchanges, tank cleaning, and health monitoring for zebrafish.
- Under general supervision, provides skilled care and husbandry for zebrafish.
- Maintain accurate, detailed records utilizing written log sheets and computer databases tracking colony health, water quality parameters, animal breeding, and housing or support areas as required.
- Responsible for limited supervisory duties including direction, new employee training and oversight of work, but excluding discipline.
- High school diploma or equivalent.
- Four years of directly related experience.
- LAT certification within one year of hire.
Preferred: Minimum of 6 months experience working with zebrafish in a research environment.
Skills: Basic computer skills and a working knowledge of computer programs such as Microsoft Outlook, Excel, and Word.
Lab Animal Technician III
Center for Comparative Medicine
BCM Main Campus, Houston
Hiring up to $41,000
Staff Job Category:
Hot Job, Research - Laboratory
ERC-funded Postdoctoral Scientist fellowship in
Laboratory of Angiogenesis and Redox Metabolism
Dept. of Biology, University of Padua, Italy
The laboratory on Prof. Massimo Santoro is seeking post-doctoral fellows to study redox homeostasis and metabolism in cancer (Panieri and Santoro, 2016). Our team has recently identified novel metabolic pathways that are crucial for tumor survival and cancer progression (more information available upon request). Such studies offer unique opprotunities for designing new therapeutic strategies against metastasis and cancer spreading.
The candidate must be a highly motivated researcher with a PhD in a relevant discipline as well as having some experience in genetic, molecular, and/or cellular biology methods. The applicant must have a good publication record in peer-reviewed international journals (including at least one paper as a first author in top-journal) and must be capable of working in a team as well as independently. Excellent communication skills in spoken and written English are required.
We offer a dynamic work environment in a stimulating scientific surrounding with a young, enthusiastic, motivated team along the opportunity to contribute to high-impact projects. We offer a strong competitive salary (European Marie Curie Fellow range) as well as contributions to health and retirement funds.
Please send your CV, letter of motivation, and the contact information of at least three references to Prof. Santoro at firstname.lastname@example.org.