Blog from July, 2017

A postdoctoral position is available in the laboratory of Dr Caroline Hill at the Francis Crick Institute (http://www.crick.ac.uk/), which is a new world-class interdisciplinary biomedical Institute situated in central London.

Work in the Hill lab is focused on understanding how TGF-β superfamily signalling pathways function normally in early vertebrate development and in adult untransformed tissue culture cells, and how these signalling pathways are perturbed in cancer. We have been exploiting the very powerful combination of early vertebrate developmental systems (primarily zebrafish embryos), together with a variety of model tissue culture systems, and we use methodologies ranging from developmental and cell biology to computational modelling. 

 

For recent publications see:

Agricola et al., 2011 Mol. Cell  43, 85–96

Wu et al., 2011, PLoS Biology 9, e1000593.

Reichert et al., 2013, Development 140, 4435–4444

Vizán et al., 2013, Sci Signal 6 ra106.

van Boxtel et al., 2015, Dev Cell 35, 175–185

Coda et al., 2017, eLife 6, 720

 

I am looking for a highly motivated postdoc with proven research abilities and an excellent publication record.

The project will focus on dissecting the regulation and function of Nodal signalling in early zebrafish embryos and determining how the Nodal pathway interacts with other signalling pathways to specify different cell fates.

 

Person Specification

 The post holder should embody and demonstrate the core Crick values: Bold, Imaginative, Open, Dynamic and Collegiate, in addition to the following:

Essential

  • PhD in a relevant area or in the final stages of completion
  •   Knowledge and experience of working with zebrafish as a developmental system.
  • Excellent track record as evidenced by publications or submitted manuscripts in refereed journals
  • A highly creative and motivated individual, able to work independently and also capable of interacting well within a group, keeping detailed records of experiments, and communicating well.

Desirable

  • Experience in signal transduction
  • Experience in imaging

 

For more details, please contact Dr Caroline Hill (caroline.hill@crick.ac.uk)

To apply please send a CV and a cover letter describing research accomplishments and interests, with the names of two to three scientists who are familiar with your research, to Dr Caroline Hill at the above email address.

The Paridaen lab at ERIBA is currently looking for a highly motivated PhD student and post-doctoral researcher.

ERIBA (The European Research Institute for the Biology of Ageing) is a newly established institute at the University Medical Centre Groningen (UMCG). The mission of ERIBA is to better understand the molecular mechanisms that result in age-related decline and age-related diseases. We aim to develop novel strategies to prevent or combat age-related diseases and to provide evidence-based recommendations for healthy ageing. The research teams are working with different model systems and technology platforms and share their knowledge and expertise to accelerate discoveries. The ERIBA is housed in a new building and is part of the central UMCG campus in the vibrant University City Groningen in the north of the Netherlands. Groningen is a small (200,000 inhabitants), but vibrant city hosting over 25,000 students.

Stem cells act in developing and adult organisms to produce the proper number of specialized cells in the body. It is important that stem cells select an appropriate division mode to ensure proper development, maintenance and repair of tissues. In the Paridaen lab, we aim to understand the mechanisms underlying stem cell decisions in developing and ageing tissues. We employ single cell analyses techniques to study stem cell behaviour in the developing brain, using zebrafish and mouse as our model systems. In particular, we are interested in the role of fate-determining factors such as signalling pathways and subcellular structures in symmetric and asymmetric stem cell divisions.

For a more extensive description and application (before September 3 2017), please visit:

http://ow.ly/NDeh30dKAxq

and: http://eriba.umcg.nl/groups/asymmetric-cell-division-and-ageing/

For more information about this vacancy you may contact:
Dr. J. Paridaen, Team leader, ERIBA; email: j.t.m.l.paridaen@umcg.nl, (please do not use emailadress for applications)

EMPLOYMENT OPPORTUNITY AT ARKANA LABORATORIES

(https://www.arkanalabs.com/research/zebrafish/)

 

Arkana Laboratories is a center for diagnostic pathology and research focused on one thing: improving care for patients.

We are seeking a senior scientist to conduct research in our state of the art zebrafish facility in Little Rock, AR.  The laboratory is developing zebrafish models of kidney disease and also performs contract research including toxicological testing.

To apply, please send your resume and letter of interest to clint.abner@arkanalabs.com

 

Job Title: Senior Scientist

Reports To: Director, Zebrafish labs

Status: Salary; Full-Time

 

Job Summary

The Zebrafish Core Program is involved in designing zebrafish disease models, provide preclinical toxicity/efficacy evaluation and provide other experimental services to industry and academic institutions, as well as internal clinical staff investigators.  The scientist should be able to provide expertise in conducting, designing and troubleshooting experiments involving zebrafish. The person should have basic knowledge of the pathophysiology of diseases.

Job Duties and Responsibilities

·         Design and conduct research.

·         Evaluate facility capabilities and work with administration to identify and develop new services.

·         Performing molecular tests

·         Design Zebrafish disease models (i.e. CRISPR, gene knockouts, etc…).

·         Act as a liaison between facility scientific staff and administration.

·         Write grants (e.g. NIH, SBIR)

·         Set and track milestones of ongoing projects as well as ensure timely delivery of the projects.

 

Performance Requirements

Overview list

·         Must be authorized to work in the United States

·         Prior experience and capability with molecular techniques in zebrafish, including,

  • Molecular techniques involving DNA, RNA and protein
  • Targeted genome editing with Cas9
  • Techniques for visualizing nucleic acids and proteins in whole mounts and tissue sections
  • Use of bioinformatic tools for primer and probe design, and sequencing data interpretation

      

Detailed list

·         Prior experience and capability with molecular techniques in zebrafish, including.

  • Nucleic acid (RNA and DNA) extraction, purification, and quantification
  • PCR techniques, including primer design, rtPCR and qPCR
  • Plasmid construction, transformation, and transfection
  • Sequencing data interpretation (NGS and direct/Sanger), including bioinformatics tools
  • Genetic manipulation

  Random gene disruption via chemical mutagenesis

  Targeted genome editing with Cas9

  • Techniques for visualizing RNA and DNA in-situ, whole mount and tissue sections
  • Protein extraction, purification, quantitation, and visualization
  • Techniques for visualizing proteins in-situ, whole mount and tissue sections
  •  Immunohistochemistry and immunofluorescence

  FISH, RNA-FISH, ISH

  • Protein extraction, purification, quantitation, and visualization

  Western blot

  • Techniques for visualizing proteins in-situ, whole mount and tissue sections   
  • Immunohistochemistry and immunofluorescence

·         Ability to troubleshoot experiments.

·         Ability to write grants.

·         Ability to lead and motivate.

·         Ability to communicate clearly, professionally, and effectively, both verbally and in writing.

·         Understanding of the legal and regulatory requirements germane to animal research.

 

Education and Experience

·         PhD in Biotechnology, Developmental Biology or Molecular Biology preferred.

·         Minimum of two years postdoctoral experience working in an academic institution or industry will be preferred.

 

Work Site Conditions

·         Working in close proximity to water and wet surfaces.

·         Moderate level of noise.

·         Potential contact with infectious agents requiring the use of Personal Protective Equipment.

 

Arkana Laboratories is an Equal Opportunity Employer

 

 

 

Follow this link to submit your application: http://uu.se/en/about-uu/join-us/details/?positionId=159868

Postdoctoral fellow in developmental biology/glycobiology

Published: 2017-07-05

Uppsala University is an international research university focused on the development of science and education. Our most important assets are all the individuals who with their curiosity and their dedication make Uppsala University one of Sweden’s most exciting work places. Uppsala University has 40,000 students, 7,000 employees and a turnover of SEK 6,5 billion.

SciLifeLab (www.scilifelab.se) is a Swedish national center for molecular biosciences with focus on health and environmental research. The center combines frontline technical expertise with advanced knowledge of translational medicine and molecular bioscience. SciLifeLab is hosted by four Swedish universities (Karolinska Institutet, KTH Royal Institute of Technology, Stockholm University and Uppsala University) and collaborates with several other universities.

SciLifeLab invites applications for Postdoc positions in the groups of our SciLifeLab Faculty.

Project description: The postdoc employed will take part in a collaborative project between Lena Kjellén at the Department of Medical Biochemistry and Microbiology and Johan Ledin at the Genome Engineering Zebrafish Facility at SciLifeLab and Uppsala University. In the project, we will apply a multiplex CRISPR/Cas9 strategy to generate zebrafish mutants where heparan sulfate sulfation varies both with regard to stage of development and localization. In addition to evaluation of the strategy, the aim is to find out how structural changes in heparan sulfate affect embryonic development. The effect of low molecular weight inhibitors of biosynthesis enzymes will also be evaluated.

Duties: Generate CRISPR/Cas9 zebrafish mutants and characterize these with regard to phenotypic changes both at organismal and molecular level. In addition to molecular biology techniques, methods for structural characterization of heparan sulfate, enzyme assays as well as advanced microscopy will be applied.

Requirements: PhD in adequate life science subject. To be eligible to apply you must have a PhD completed within three years of the application deadline. If you received your PhD earlier but special circumstances exist you are also eligible to apply. Excellent spoken and written English is required. You must be able to work both independently as well as a part of a team. Personal characteristics, such as high degree of responsibility and ability to work with others will be important selection factors.

Additional qualifications: Previous experience of working with zebrafish as a model system. Good experimental and theoretical knowledge of molecular biology techniques. Research in the glycobiology area.

Uppsala University strives to be an inclusive workplace that promotes equal opportunities and attracts qualified candidates who can contribute to the University’s excellence and diversity. We welcome applications from all sections of the community and from people of all backgrounds.

Pay: Individual salaries

Starting: October 1, 2017 or as othewise agreed.

Type of employment: Temporary position for a period of two years.

Working hours: 100 %

For further information about the position please contact: Lena Kjellén, +46 70 5833768, lena.kjellen@imbim.uu.se or Johan Ledin, +46 70 2132329, johan.ledin@scilifelab.uu.se

You are welcome to submit your application no later than August 21, 2017, UFV-PA 2017/2641.

Are you considering moving to Sweden to work at Uppsala University? If so, you will find much information about working and living in Sweden at www.uu.se/joinus. You are also welcome to contact the International Faculty and Staff Services at ifss@uadm.uu.se.

We decline offers of recruitment and advertising help. We only accept the application the way described in the advertisement.

Placement: Department of Medical Biochemistry and Microbiology
Type of employment: Full time , Temporary position longer than 6 months Pay: Fixed pay
Number of positions: 1
Working hours: 100%
Town: Uppsala
County: Uppsala län

Union representative: Per Sundman, Saco-rådet 018-471 1485 Suzanne Borén Andersson, TCO/ST 018-471 6251
Ellena Papaioannou, Seko 018-471 3315

Number of reference: UFV-PA 2017/2641 Last application date: 2017-08-21 

Postdoctoral Research Fellow in retinal regeneration – University of Texas Health Science Center at Houston

 

The O'Brien lab is recruiting a motivated postdoctoral researcher to study the molecular basis of replacement of retinal neurons from progenitor cells in the degenerating retina.  The successful applicant will join an interdisciplinary research team employing unique animal models, genetic techniques, and modern molecular techniques.  The University of Texas Health Science Center at Houston hosts a vibrant research community with strengths in neuroscience and regenerative medicine.  The rich intellectual environment is supplemented by formal career development programs and training opportunities for post-docs.

Candidates should have a recent Ph.D. or M.D./Ph.D. degree, a published record of research productivity, and an ability to design and troubleshoot experiments.  Experience with zebrafish is required and experience with retinal progenitor cells or development is preferred.  Competitive salary and benefits are provided.  Please send CV, a cover letter describing research accomplishments and interests, and have two to three scientists who are familiar with your research send letters of recommendation to: 

 

John O'Brien, Ph.D.

Professor

Department of Ophthalmology & Visual Science

McGovern Medical School of the University of Texas, Houston

6431 Fannin St., MSB 7.024,

Houston, TX  77030

(713) 500-5983

 

E-mail:  John.OBrien@uth.tmc.edu

 

UTHealth does not discriminate on the basis of race, color, religion, sex, sexual orientation, national origin, age, disability, genetic information, gender identity or expression, veteran status, or any other basis prohibited by law or university policy.

EOE/M/F/Disabled/Vet

 

PhD position in the mechanics of neuronal development

 

A 3-year funded PhD position is available at the Institut de Biologie Paris Seine to investigate the role of mechanical forces in the construction of a neuronal circuit in vivo.

During neuronal circuit formation, neurons move towards their final location while growing axons towards their target. While the biochemical guidance cues involved in neuronal migration and axon elongation are extensively studied, the contribution of mechanical forces in these processes remains largely unexplored in vivo

In the lab we address this question using the zebrafish olfactory circuit as a model system. Its location underneath the skin of the embryo makes it amenable to live imaging and mechanical perturbation. We already obtained imaging and functional data suggesting an important function for mechanical cues in the formation of the circuit: olfactory axons extend through the effect of extrinsic mechanical forces that drive the passive displacement of neuronal cell bodies away from their axon tips (Breau et al., Nat Comm, in press).  

The purpose of the PhD project is to further identify the origin and contribution of mechanical forces in the construction of the circuit, and the molecular mechanisms involved in force propagation and sensing. To achieve this goal, the student will use a pluridisciplinary strategy combining multiscale live imaging, genetic/optogenetic tools and physical approaches to measure and perturb forces in vivo. 

We are looking for a highly motivated student willing to join an interdisciplinary environment involving strong interactions between biologists and physicists.

 

Requirements

- Master degree in cell/developmental biology or in biophysics 

- Strong interest towards interdisciplinary work

 

Additional beneficial skills:

- Experience with zebrafish 

- Skills in confocal, biphoton or light sheet microscopy

- Experience in image analysis (Image J, Matlab)

 

Starting date: between October 2017 and January 2018. To apply, please send your CV and references to Marie Breau, Institut de Biologie Paris Seine:  

marie.breau@upmc.fr

A PhD student position is available in the laboratory of Prof. Matthias Hammerschmidt at the Biocenter of the University of Cologne, Germany, to contribute to our ongoing research on bone development and homeostasis in the zebrafish. The project will focus on the vertebral column. One part will deal with the roles of different growth factors (retinoic acid, BMP, FGF, Wnt etc.) and their interconnection during spine development at larval stages, the other with mutants isolated during our in-house forward genetics displaying scoliosis in juvenile and adult fish / post metamorphosis. The causative recessive mutation of one of the scoliosis mutants has been resolved, requiring confirmation via CRISPR/Cas genetics, as well as further in-depth phenotypic analyses, including state of the art imaging technologies including multiphoton confocal microscopy and microCT.

For further information, please visit our website (http://www.ag-hammerschmidt.zoologie.unikoeln.de/3.html) or contact us directly via email or telephone (see below).

The Cologne Biocenter is located in the center of Cologne, a very international and lively city, and offers an excellent scientific environment for the conducted research. The University of Cologne has been awarded the status of a “University of Excellence”, with our research group as part of the funded Cluster of Excellence CECAD. In addition, we are part of the Center for Molecular Medicine Cologne (CMMC).

 

Previous experience with the zebrafish is preferred, but not essential. Proficiency in English is required. To apply, please email your detailed CV and names of at least two referees to:

 

Hans-Martin Pogoda (senior scientist and Co-PI in Hammerschmidt lab)

pogodah@uni-koeln.de; phone: ++49-221-470 2738

Cologne University, Biocenter - Room 3-502, Zuelpicher Strasse 47b,

D-50674 Koeln, Germany

PhD student position on zebrafish skin development, regeneration or carcinogenesis in Cologne, Germany

A second PhD student position is available in the laboratory of Prof. Matthias Hammerschmidt at the Biocenter of the University of Cologne, Germany, to contribute to our ongoing research on skin development, regeneration or carcinogenesis in the zebrafish. Using a combination of forward and reverse (CRISPR) genetics, transgenesis, pharmacogenetics, and in vivo imaging, we study different aspects of skin development, including cornification-like processes in epidermal appendages named breeding tubercles, as well as epithelial carcinogenesis and wound healing in the adult skin, including the formation and regression of scar tissue (see for instance, Richardson et al., Development 2016; Hatzold et al., eLife 2016). Our research is of high biomedical relevance, as several of our investigated genes are also implicated in human skin development, homeostasis or disease.

For further information, please visit our website (http://www.ag-hammerschmidt.zoologie.unikoeln. de/3.html) or contact us directly via email or telephone (see below).

The Cologne Biocenter is located in the center of Cologne, a very international and lively city, and offers an excellent scientific environment for the conducted research. The University of Cologne has been awarded the status of a “University of Excellence”, with our research group as part of the funded Cluster of Excellence CECAD. In addition, we are part of the Center for Molecular Medicine Cologne (CMMC) and the Collaborative Research Center (SFB829) entitled “Molecular Mechanisms regulating skin homeostasis”, with over 20 participating research groups working on different aspects of non-mammalian, mouse or human skin development and disease.

Previous experience with the zebrafish is preferred, but not absolutely essential. Proficiency in English is required. To apply, please email your detailed CV and names of at least two referees to:

 

Matthias Hammerschmidt

mhammers@uni-koeln.de; phone: ++49-221-470 5665

Cologne University, Biocenter - Room 3-502, Zuelpicher Strasse 47b,

D-50674 Koeln, Germany

A postdoctoral fellow position is available for a creative and highly motivated scientist in the research group of Dr. Chris Hall in the Zebrafish Development and Disease Models Laboratory at the University of Auckland, to study macrophage activation during acute gouty inflammation.

 

Project description:

Acute gouty inflammation is the result of monosodium urate (MSU) crystal deposition in and around peripheral joints leading to activation of tissue-resident macrophages, neutrophil recruitment and acute joint inflammation. There is a clinical need for new anti-inflammatory treatments for gout as current therapies are non-specific and limited by potential drug toxicities, and gaps in therapeutic efficacy. The primary aim of this project is to leverage off a unique larval zebrafish model of MSU crystal-driven inflammation we have developed, to provide new insights into how macrophages are activated by MSU crystals and uncover new drugs to alleviate acute gouty inflammation.

Dr Hall’s research group has an established track record of exploiting the zebrafish model system to provide unique insights into innate immune cell function and as a drug discovery platform (see Astin et al., Methods Cell Biol 2017, Hall et al., Nature Communications 2014, Hall et al., Dis Model Mech 2014, Hall et al., Cell Metabolism 2013, Hall et al., Cell Stem Cell 2012, Hall et al., BMC Dev Biol 2007).

 

Position availability:

From 1st November 2017 (funded by a 3 year grant from the Health Research Council of New Zealand).

 

Applicant qualifications:

Candidates must have a PhD (ideally within the last 4 years) and show evidence of exceptional scientific contributions through publication in high impact journals, ideally in the fields of blood development and/or immunity. Candidates with experience in live cell imaging, standard and advanced cellular and molecular biology approaches, genetics, biochemistry, innate immunity and blood development will be viewed favourably. Although not essential, experience with the zebrafish model system and generating CRISPR mutants is desirable.

 

Please send an academic CV and names and contact information of 3 referees to (closing date, 31st August 2017):

 

Chris Hall (PhD)

Senior Lecturer in Immunology

School of Medical Sciences

Faculty of Medical and Health Sciences

University of Auckland

Private bag 92019

Auckland 1142

New Zealand

Email: c.hall@auckland.ac.nz

Phone: +64 9 923 2910

 

For more information about the Hall group visit:

https://www.fmhs.auckland.ac.nz/en/sms/about/our-departments/mmp/our-research/zebrafish-development-and-disease-models.html#aedd42755db6d048ce2066e44f4ef294

 

 

Post Title: Postdoctoral Research Fellow in Sensory-Motor Interactions

School/department: School of Life Sciences and the School of Engineering and Informatics

           

Hours: full time

Contract: fixed term for 3 years

Reference
Salary: £35,609 per annum

Closing date:  31st August. 

Expected Interview date: Mid September

Expected start date: November 1st

  

A post-doctoral position is available jointly in the groups of Prof. Leon Lagnado (School of Life Sciences) and Dr Christopher L Buckley (School of Engineering and Informatics).  This collaborative, BBSRC funded, project aims to investigate how self-generated visual input is processed in the vertebrate brain. Light-sheet microscopy will be used to image neural activity across the brain of live zebrafish behaving in a virtual reality environment in order to characterise the neural circuits involved in the interactions between the sensory and motor systems (an example of the imaging technique to be used can be found in Nature. 2013;493: 466–468).  The position would suit an experimental neuroscientist who has strong interests in collaborating with computational and systems neuroscientists.

Sussex Neuroscience (http://www.sussex.ac.uk/sussexneuroscience) is one of the foremost centres for Neuroscience research in the UK and the University of Sussex has a beautiful campus on the outskirts of the lively South Coast town of Brighton.

Applicants must have a Ph.D and research experience in neuroscience. A quantitative approach is essential and experience in zebrafish neurobiology would be a distinct advantage. An overview of the project is available at https://tinyurl.com/kjtxoq9. Informal enquiries are encouraged and should be made to Leon Lagnado (l.lagnado@sussex.ac.uk) or Chris Buckley (c.l.buckley@sussex.ac.uk).

 

 

The University of Mississippi School of Pharmacy, Department of BioMolecular Sciences is searching for a Postdoctoral Research Associate in the field of Molecular Toxicology for a position that is available immediately. The University of Mississippi is located in Oxford, MS, which is commonly rated among the top college towns in the country. The School of Pharmacy is ranked in the top ten nationally for research funding, and is home to the National Center for Natural Products Research and the Center of Research Excellence in Natural Products Neuroscience. Applications must be submitted online at jobs.olemiss.edu. Applicants should provide a cover letter outlining qualifications for the position, a curriculum vitae, and the name and contact information of three references. Review of applications will begin immediately, and continue until the position is filled.

 
The successful candidate will join an active NIH­ funded research program coupling developmental and molecular toxicology. The goal of the project is to compare the relative morphological, behavioral, reproductive and multigenerational phenotypes that result after developmental exposures to cannabidiol (CBD) and Δ9-tetrahydrocannabinol (THC). The candidate will use zebrafish as the animal model and assess phenotypic alterations caused by exposures and mechanistically link the adverse outcomes with targeted and global transcriptomic analyses.

 
A Ph.D. in Toxicology or a related field is required by the time of the appointment. A strong research background with multiple quality publications is required. Candidates should have experience in transcriptomic analysis and conducting dose-dependent toxicity assays.

 
The University of Mississippi is an EOE/AA/Minorities/Females/Vet/Disability/Sexual Orientation/Gender Identity/Title VI/Title VII/Title IX/504/ADA/ADEA employer.

The Torres-Vázquez lab studies the formation, remodeling and function of the vascular system at the cellular and molecular levels using zebrafish and cultured cells. 

We are seeking Postdoctoral researchers interested in studying these (and other) topics:

1. Patterning of angiogenic development

2. Vascularization of the central nervous system (CNS) and blood-brain barrier (BBB) differentiation

3. Mechanisms of vessel assembly and lumenization

 

Position availability: late 2017, early 2018.

Links of interest: https://scholar.google.com/citations?hl=en&user=eTBw3VIAAAAJ&view_op=list_works&sortby=pubdate

 

Applicant qualifications:

Exceptional publication record

Experience in standard and advanced molecular biology approaches

Experience with model organisms is preferred.

Practical knowledge of genetics, cell biology, cell culture, biochemistry, bioinformatics, genomics, cell-type specific profiling of gene expression and imaging is a plus.

 

Please send or email CV and reprints of relevant publications to the contact information below using the subject line: Postdoctoral applicant: Your name.

We encourage applicants to outline specific research projects. Applicants accepted for initial consideration will be contacted for letters of reference. 

 

Jesús Torres-Vázquez, Ph.D.

jtorresv@med.nyu.edu