Postdoctoral position to study Wnt signaling and embryonic cell polarity.
A postdoctoral position is available in the Sergei Sokol laboratory in the Department of Cell, Developmental and Regenerative Biology, Icahn School of Medicine at Mount Sinai, New York, to study Wnt signaling and cell polarity during early vertebrate development using Xenopus and zebrafish embryos and mammalian progenitor cells. We are especially interested in cell behaviors during neural tissue closure and the mechanisms of skin and neural crest specification. See our recent papers and the description of our interests at http://research.mssm.edu/sokol/Sokol_lab/ .
Preference will be given to highly motivated applicants with strong background in molecular and cellular biology, who published a first-author paper as a result of their graduate work. Interested candidates may send their CV, list of publications and three references to Dr. Sergei Sokol (sergei.sokol@mssm.edu).
Laboratory Technician (m/f)
Fixed-term contract 2 years, 40h/week, with possibility of becoming permanent
Employee status (start date: 15th February 2017)
Reference: R-AGR-0356-12-C
Your Role
Area: the applicant will be a member of the Chemical Biology group (Head: Dr. Alexander Crawford). The main objective of the group is to use small molecules to give insights into the aetiology of disorders of the human central nervous system and to find possible future therapies. The group’s research is centred on the use of zebrafish as an in vivo disease model.
The laboratory technician (m/f) will contribute to ongoing research projects and assist in the maintenance of the aquatic platform at LCSB.
Your tasks will include:
- Maintenance of the aquatic facilities, e.g. daily duties (breeding, feeding, control of water parameters)
- Performance of experiments using zebrafish embryos/larvae, e.g. microinjections, behavioral tests
- Performance of zebrafish-based small molecule screens
- Use and maintenance of various automated systems and equipment (robotic feeding system, tank washer, automated microinjection system, automated behavioral tracking systems)
- Extraction of DNA, RNA, proteins, metabolites for subsequent analyses (e.g. -omics analysis)
- Performance of molecular biology experiments such as Tol2 transgenesis, CRISPR mutagenesis, PCR-based genotyping
- Contribution to general laboratory organization, e.g. preparing and maintain stock/working chemical solutions; ordering, making inventories and keeping stock supplies for laboratory use; etc.
- Maintenance of a clean laboratory setting
- Keeping records of the projects in a well-organized laboratory notebook
- Use and maintenance of a laboratory information management system (LIMS)
- Participation in technical meetings with other technicians at LCSB
Your profile
- A technical degree or equivalent in biology, molecular biology, biochemistry, bioengineering, or similar
- Experience with molecular biology and microscopy is essential
- Excellent organizational skills
- Experience with biochemical assays will all be considered as an advantage
- Experience with working with animals, particularly fish species, will be considered as an advantage
- Good computer skills for handling common software (Excel, Word, PowerPoint, LIMS, etc.)
- Flexible regarding research stays in collaborators’ research laboratories
- Excellent working knowledge of English is essential, knowledge of German and/or French is an asset
We offer
- Full contract for 2 years with the possibility to become a permanent staff at the LCSB
- Opportunity to work in an interdisciplinary, international environment
- Opportunity to participate in the development of the group’s research
- Opportunity for professional trainings
- Opportunity to work in a new, modern aquatic facility
Further Information
Applications (in English) should contain the following documents:
- A detailed Curriculum vitae
- A motivation letter that includes a description of previous professional experiences
- Please ask at least two references to email their confidential letters directly to Maria Lorena Cordero Maldonado (marialorena.corderomaldonado@uni.lu) within two weeks of submitting the application.
Review of applicants will begin immediately and will continue until the position is filled.
The University of Luxembourg is an equal opportunity employer. All applications will be treated in the strictest confidence.
Please apply online until 15th January 2017 via this link: http://emea3.mrted.ly/198p7
Only applications submitted via this link will be considered.
Postdoctoral Scientist in Fish Heart Regeneration
Department of Physiology, Anatomy and Genetics, Oxford
Grade 7: Salary in the range £31,076 - £32,004 p.a.
Applications are invited for a Postdoctoral Research Scientist to join Professor Mathilda Mommersteeg’s laboratory to work on a project focussed on identifying the mechanisms underlying fish heart regeneration using the Mexican cavefish, Astyanax mexicanus. The post would be ideally suited to a postdoctoral scientist with experience in working with fish models and background expertise in heart development or regeneration.
The successful applicant will be specifically charged with performing QTL analyses to link the ability for heart regeneration to the genome, followed by functional validation of the identified loci. The main techniques in this project will include fish cardiac injury surgery, the generation of constructs for injection to generate transgenic zebrafish and Astyanax mexicanus lines using CRISPR, oocyte injections, western blotting, qRT-PCR, immunohistochemistry and in situ hybridisation.
You must hold, or be near completion of a PhD (or equivalent) in molecular biology, biomedical sciences, genetics or a related science and have experience of working with in vivo models of development and/or cardiovascular disease.
You will be based in the Sherrington Building in the Department of Physiology, Anatomy and Genetics at the University of Oxford.
The position is funded by an ERC starting grant for up to 5 years. The post is due to start on 1 March 2017.
The closing date for applications is 12.00 noon on Monday 19 December 2016. Interviews are likely to be held early January 2017.
More information on the website: https://www.recruit.ox.ac.uk/pls/hrisliverecruit/erq_jobspec_version_4.jobspec?p_id=126188
Position Title: Biologist post doctoral fellow in tumor microenvironment
Position Description:
A fully funded postdoctoral position is available to study tumor microenvironment in at the National Cancer Institute on the NIH main campus in Bethesda, MD. Specifically, this opening is for an ongoing project examining the role of tissue architecture and mechanotransduction in the establishment of metastatic lesions, using zebrafish as a model system.
Techniques to be used include tumor cell culture, orthotopic Danio rerio or murine tumor modeling, microscopy, standard molecular biology protocols, and bioinformatics. The NIH will provide Funding and benefits, though extramural fellowship applications will be strongly encouraged and supported. PhD in a relevant subject area, Ability to work in a team, and communicate ideas and results, demonstration of motivation and technical accomplishments by high quality, first author publication(s), Proven independent working style, problem solving, data analysis and generation of novel ideas, advanced knowledge of molecular biology and biochemistry, experience with next generation sequence data generation and analysis or advanced knowledge of molecular biology and genetic engineering tools. Desired Skills include previous experience with genetic analysis/engineering in a model organism, in vivo imaging and image analysis
Requirements: Applicants should possess a Ph.D. and have a strong background in molecular biology and/or cell biology and a minimum of one first-authorship manuscript in a peer-reviewed journal. Experience in cancer biology or cell biology is preferred. Applicants should have no more than one year of postdoctoral training. Appointment duration is up to 5 years.
To Apply: Interested individuals should e-mail a cover letter, curriculum vitae, bibliography, and contact information for 3 references to:
Dr. Kandice Tanner
Investigator, Laboratory of Cell Biology
Center for Cancer Research
National Cancer Institute
37 Convent Drive
Bldg. 37, Rm. 2132
Bethesda, MD 20892
U.S.A.
E-mail: kandice.tanner@nih.gov
Web page: https://ccr.cancer.gov/Laboratory-of-Cell-Biology/kandice-tanner
This position is subject to a background investigation. The NIH is dedicated to building a diverse community in its training and employment programs.
Employer Name: National Cancer Institute
Position Location: Bethesda, MD
The Langenau Laboratory at the Massachusetts General Hospital, Boston is recruiting a research fellow to study the role of a novel HMG box protein in regulating DNA repair and leukemogenesis. Research will focus on using cutting edge proteomics, biochemistry, cell biological assays, and zebrafish animal modeling.
Dr. Langenau’s research group has become a pioneer in the field pediatric cancer research. The laboratories interests are best summarized in the following manuscripts (Moore et al., JEM, 2016; Tang et al., Nature Communications, 2016; Blackburn et al., Cancer Cell 2014; Tang et al., Nature Methods, 2014; Chen et al., PNAS 2014; Ignatius et al., Cancer Cell 2012). Additional information about the laboratory is available at langenaulab.com.
Applicants with advanced skills in zebrafish genetics, DNA repair, proteomics, biochemistry, and cell biological assays (including ChIP seq) are highly desired.
Candidates must have PhD and/or MD, have made significant scientific contributions through publication of high impact papers, and be enthusiastic about science.
A curriculum vitae, list of publications, and three references should be provided as a single PDF by December 20th, 2016 to:
David Langenau
Molecular Pathology Unit
Massachusetts General Hospital
149 Thirteenth Street, Room 6133
Charlestown, MA 02129
The Langenau laboratory research focus is to uncover the mechanisms driving progression and relapse in pediatric tumors. Using novel, transgenic zebrafish models of pediatric sarcoma and leukemia that mimic human malignancy, we have undertaken studies to discover novel therapies by addition of drugs to the water and imaging tumor growth in live zebrafish. Moreover, we have utilized detailed imaging studies to visualize tumor cells in live animals and assess how cellular heterogeneity drives continued tumor growth. Capitalizing on insights gained from our zebrafish models of cancer, we are now extending our findings to human T-cell acute lymphoblastic leukemia and rhabdomyosarcoma.
Massachusetts General Hospital, a leading medical center located in the heart of Boston, is consistently ranked among the top hospitals in America. The original and largest teaching hospital of Harvard Medical School, Mass General conducts the largest hospital-based research program in the United States and is the top recipient of research funding from the National Institutes of Health (NIH). Mass General has long been a leader in successfully bridging innovative science with state-of-the-art clinical medicine and topped the Nature Index list of health care organizations for publishing more articles in high-impact journals than any other hospital in America from February 2015 to January 2016.
Postdoctoral position with competitive salary including all social benefits available at BioMediTech, an independent research institute with brand new building and top end infrastructure at the University of Tampere, Finland. Extra financial aid to facilitate moving can be discussed. Deadline 15.12.2016.
Scope of Research:
(1) Transfer novel CRISPR-Cas9 based system which allows elevation of strictly endogenous gene expression recently set up by the lab in mouse into zebra fish system.
(2) Generate novel Parkinson’s disease and dopamine system regeneration models using the above novel and conventional approaches.
(3) Analyze “hits” from omics screens in mouse dopamine system function and regeneration generated by other lab members in zebrafish system.
We are recruiting an outstanding candidate with expertise in molecular biology, strong drive and an ability to conduct independent research.
The post-doc will have his/her own technician (50% time allocation) with expertise in fish genetics, analysis and zygote injections. After settling in he/she will supervise 1-2 MSc students who will work 100% on the project(s).
Experience in fish or mouse development, embryo or cell imaging tools, bioinformatics and/or Parkinsons’s disease models would be desirable but not required. The required key expertize is molecular biology and a strong ambition and drive.
The Andressoo group investigates how ability to change endogenous morphogen levels – neurotrophic factors, mitochondrial quality control genes, chaperones, etc impact the outcome in development and in models of Parkinson’s disease with a long-term goal to extend the concept into other neurodegenerative conditions and CNS traumas. The main model organism has been mouse, but the lab seeks to set up research in zebrafish. The main lab is currently situated at the University of Helsinki, Institute of Biotechnology, the new line of research in zebra fish is set up at BioMediTech, University of Tampere, where JO Andressoo was recently nominated as Associate Professor in Cell and Tissue Engineering. The plan is to gradually transfer the lab from Helsinki to Tampere.
Recent publications
1. Kumar A, Kopra J, Varendi K, Porokuokka LL, Panhelainen A, Kuure S, Marshall, P, Nevalainen N, Härma MA, Vilenius C, Lilleväli K, Tekko T, Mijatovic M, Pulkkinen N, Jakobson Mad, Jakobson Mai, Ola R, Palm E, Lindahl M, Strömberg I, Võikar V, Piepponen TP, Saarma M, Andressoo JO . GDNF overexpression from the native locus reveals its role in the nigrostriatal dopaminergic system function. PLOS Genetics, 2015 Dec 17;11(12).
2. Kopra J, Vilenius C, Grealish S, Varendi K, Lindholm J, Castren E, Voikar V, Björklund A, Piepponen P, Saarma M, Andressoo JO. GDNF is not required for catecholaminergic neuron survival in vivo. Nature Neuroscience, 2015 Mar;18(3):319-22.
The lab has several manuscripts under revision or under submission.
Please provide a CV, statement of interest, and the names of 2-3 references.
Jaan-Olle Andressoo
Cell and Tissue Engineering
Associate Professor
University of Tampere
Principal Investigator
Institute of Biotechnology
P.O. Box 56, Viikinkaari 5D
FI-00014 University of Helsinki, Finland
Tel. +358-2941 59394
GSM +358 503581213
jaan-olle.andressoo@helsinki.fi
http://www.biocenter.helsinki.fi/bi/andressoo/