Research Specialist - Johns Hopkins University
OVERVIEW: We are seeking individuals interested in a Research Specialist position in a biological laboratory focused on Regenerative Biology. Appropriate candidates should be proficient in modern molecular biology techniques. In addition, individuals with prior experience with the zebrafish model system are highly sought.
RESEARCH SUMMARY: In the Mumm laboratory (Wilmer Eye Institute, Johns Hopkins University), the central goal of our research is to understand how specific retinal neuron subtypes can be regenerated. We utilize zebrafish for our studies due to significant advantages afforded by this model system. The most important advantage being that zebrafish have a robust capacity for regeneration. We combine cell-specific ablation and labeling methods with high-resolution 4D imaging of the living retina to make movies of how cell types of interest (e.g., stem cells, immune cells) respond to the loss of retinal neurons. In addition, we use large-scale genetic and chemical screening platforms to identify genes and signaling systems that control the regenerative potential of a retinal stem cell, called Müller glia. Our ultimate goal is to reveal therapeutic strategies for harnessing the regenerative capacities of human Muller glia cells; thus, promoting the replacement of degenerated cell types as a curative approach for patients with blinding retinal diseases.
RESPONSIBILITIES: This position requires a thorough understanding and vigilant implementation of the scientific method. The Specialist will be expected to fully comprehend the theory behind experiments and apply critical analytical skills during assigned projects in order to identify key findings and trouble-shoot problems. Meticulous attention to detail is a critical skill that the ideal candidate must have. More specifically, the primary responsibility of this position will be to assist postdoctoral fellows in conducting experiments analyzing chemical compounds and genetic mutations for the ability to alter the kinetics of retinal regeneration. Examples of responsibilities include: 1) preparation of recombinant DNA plasmids, 2) creation of transgenic zebrafish lines, and 3) operate and maintain a robotic high-throughput screening system. Additional duties include: 1) assist in the training of graduate/undergraduate students, 2) help to maintain and monitor a large Zebrafish facility, and 3) maintain and monitor shared resources.
QUALIFICATIONS: Bachelor’s degree in Biological Sciences or related scientific field. Two years of previous research related work experience is required, in particular expertise in the following techniques/experiences is sought: Molecular Biology, in particular plasmid sub-cloning; Transgenesis; High-resolution imaging (confocal microscopy); Histological and immunological tissue processing. The ideal candidate will have had prior experience with zebrafish (e.g. general care, breeding, genotyping, phenotyping, chemical biology) and zebrafish-related techniques (e.g. egg microinjection, CRISPR/Cas9 design). The candidate must be able to work both independently and in a collaborative manner with a diverse group of researchers.
LOCATION: Rm. 4001-G, Smith Building; 400 North Broadway; Johns Hopkins University; Baltimore, MD; 21231
CONTACT: Meera Saxena (msaxena1@jhmi.edu), subject line “Research Specialist”. Please provide current resume / CV.