Research Assistant 1 - Yale University

Yale University offers exciting opportunities for achievement and growth in New Haven, Connecticut. Conveniently located between Boston and New York, New Haven is the creative capital of Connecticut with cultural resources that include three major museums, a critically-acclaimed repertory theater, state-of-the-art concert hall, and world-renowned schools of Architecture, Art, Drama, and Music.

General Purpose: Generates and maintains lines of zebrafish mutants of autism risk genes and transgenic lines carrying these mutations; screens and genotypes zebrafish using PCR. Identifies mutations by cloning and sequencing. Confirms protein loss in mutants by western blotting. Analyzes CNS development and brain structure in zebrafish mutants. Dissects zebrafish brains; performs whole mount immunocytochemistry and in situ hybridization; performs confocal imaging. Analyzes locomotor activity of zebrafish mutants using automated tracking software. Develops and codes MatLab programs to analyze zebrafish behavior. Conducts and analyzes high-throughput drug screening experiments in zebrafish mutants. Analyzes gene expression in the brains of zebrafish mutants and in specific cell types using FACS sorting and RNA-Seq. May assist in designing and developing activity-based brain imaging assays using two-photon imaging. Orders and maintains inventory of supplies. May assist research and support staff.

Required Education and Experience: Six years of related work experience, four of them in the same job family at the next lower level, and high school level education; or four years of related work experience and an Associate's degree; or little or no work experience and a Bachelor's degree in a related field; or an equivalent combination of experience and education.

Qualifications:
Proficiency with molecular biology techniques, PCR, cloning, and genotyping.
Demonstrated experience with immunohistochemistry, whole mount in situ hybridization, micro-dissections. Proven experience with fluorescence microscopy and confocal imaging.
Proven work with animal models of human disease and knowledge of human genetics.
Demonstrated experience with automated behavior tracking assays, analysis, and drug screens.
Proven experience with western blotting and RNA-Seq.
Preferred: Five years of related work experience and a Bachelor's degree in a related field; or an equivalent combination of experience and education. Preferred skills include: prior work with zebrafish; knowledge of MatLab coding; experience with FACS sorting and two-photon imaging.

Application: For more information and immediate consideration, please apply online at www.yale.edu/jobs - the STARS req ID for this position is 28804BR. Please be sure to reference this website when applying for this position.

We invite you to discover the excitement, diversity, rewards and excellence of a career at Yale University. One of the country's great workplaces, Yale University offers exciting opportunities for meaningful accomplishment and true growth. Our benefits package is among the best anywhere, with a wide variety of insurance choices, liberal paid time off, fantastic family and educational benefits, a variety of retirement benefits, extensive recreational facilities, and much more.

Yale University considers applicants for employment without regard to, and does not discriminate on the basis of an individual’s sex, race, color, religion, age, disability, status as a veteran, or national or ethnic origin; nor does Yale discriminate on the basis of sexual orientation or gender identity or expression.