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Assays Tested

Assay

Prep

Worked

Notes

immunohistochemistry

yes

from ZFIN curation

Notes

  • Imported from ZFIN Antibody page zpr-1
  • labels green/red double cones (Zou, J., Lathrop, K.L., Sun, M., and Wei, X.)
  • Hybridomas were produced by immunization of male Balb/c mice at multiple intraperitoneal and subcutaneous sites with a preparation of whole adult retinal cells that had been fixed in 2 % paraformaldehyde for 30 minutes at room temperature and resuspended in PBS and Freund's adjuvant. Each immunization consisted of retinal cells obtained from approximately fifteen adult wild-type zebrafish. Each mouse received at least nine immunizations over the course of approximately one year. The cell fusion and cloning of hybridomas were completed by the methods described by Ciment and Weston (1982) and modified by Trevarrow et al. (1990). (Larison, K.D. and BreMiller, R.)
  • Citations for zpr-1 at ZFIN