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Assays Tested

Assay

Prep

Worked

Notes

immunohistochemistry

yes

from ZFIN curation

Notes

  1. zm4 was made by Monte Westerfield against a synthetic peptide from a transcription factor expressed in the brain (dlx2?). zm4 does NOT work on paraformaldehyde fixed tissue, but works very reliably on alcohol-fixed tissue (we use Carnoy's Fix).  Labeling does not seem to be myofibrillar.