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Assays Tested

Assay

Prep

Worked

Notes

immunohistochemistry

yes

from ZFIN curation

western blot

yes

from ZFIN curation

Notes

  • Imported from ZFIN Antibody page Ab3-h2afx
  • Immunogen: Peptide sequence around phosphorylation site of serine 139 (Q-A-S(p)-Q-E) derived from Human Histone H2A.X. (ZFIN Staff)
  • The antibody detects endogenous levels of histone H2A.X only when phosphorylated at serine 139. (ZFIN Staff)
  • Citations for Ab3-h2afx at ZFIN