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Assays Tested

Assay

Prep

Worked

Notes

Notes

  • Imported from ZFIN Antibody page Ab1-pum1
  • N terminus fragment of Xenopus Pum1 (amino acids 1–410) was expressed in Escherichia coli, gel-purified, and injected into two rabbits to produce antibodies. A GST-fused N terminus fragment of zebrafish Pum1 (amino acids 1–383) was expressed in E. coli, electroblotted onto a membrane (Immobilon; EMD Millipore), and used to affinity purify the rabbit antisera. (Kotani, T., Yasuda, K., Ota, R., and Yamashita, M.)
  • Citations for Ab1-pum1 at ZFIN