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Antibody Name:

Ab1-pbx from the ZFIN antibody database.

Other names, clone ids, catalog ids etc.

anti-pan-PBX , pbx

Does it work on zebrafish?

yes

Host organism

Rabbit

Immunogen organism

Zebrafish

Antibody isotype

Antibody type

polyclonal

Anatomical structures recognized
(use terms from the ZFIN Anatomical Ontology)

whole organism

Recognized target molecules (gene names, domains, epitopes ...)

pbx1b  pbx2  pbx3b  pbx4  

Suppliers

Assays Tested

Assay

Prep

Worked

Notes

western blot

yes

from ZFIN curation

Notes

  • Imported from ZFIN Antibody page Ab1-pbx
  • Pbx1b, Pbx3b, and Pbx4 proteins containing a 6xHis affinity tag at their N terminus were expressed in E. coli SG13009 [pREP4] induced with 0.5 mM IPTG for 3 hr at 30 C. Soluble His-tagged Pbx proteins were purified under native conditions using Ni-NTA agarose (Qiagen) and were used to immunize one rabbit (150 ug of recombinant protein per injection, initial immunization with Pbx3b, first boost with Pbx4, second boost with Pbx1b, 3rd boost with Pbx3b and Pbx4). The pan-Pbx rabbit antiserum was further affinity purified using GST-Pbx3b coupled to cyanogen bromide-activated Sephrose 4B(Sigma). The affinity-purified pan-Pbx antibodies detect four zebrafish Pbx proteins (Pbx1, Pbx3, Pbx4, and Pbxy [now named Pbx2] ) equally well on a Western blot (data not shown). (Pöpperl, H., Rikhof, H., Chang, H., Haffter, P., Kimmel, C.B., and Moens, C.B.)
  • Citations for Ab1-pbx at ZFIN