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Assays Tested

Assay

Prep

Worked

Notes

immunohistochemistry

yes

from ZFIN curation

western blot

yes

from ZFIN curation

Notes

  • Imported from ZFIN Antibody page Ab1-fscn2
  • Lin-Jones and Burnside (2007) generated fascin 2 antibodies using maltose-binding protein (MBP) fusions to Xenopus Fscn2, Danio rerio Fscn2A, and Danio rerio Fscn2B were expressed in BL21 cells and purified with amylose resin. A mixture of the three purified proteins was injected into rabbits to produce polyclonal antisera (Bethyl Laboratories, Montgomery, TX). (Lin-Jones, J., and Burnside, B.)
  • Citations for Ab1-fscn2 at ZFIN