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Assays Tested

Assay

Prep

Worked

Notes

immunohistochemistry

yes

from ZFIN curation

Notes

  1. Hello,

    I just bought ab 14370, an anti Hu antibody from Abcam.  Immunostianing tissues fixed with 4% PF didn't work- whole mounted tissues is what I used, adults.  I have had success in the past.  Does anyone know if there are issues with particular lot numbers? 

    I'll try this again, using larvae I suppose...

    thanks for your help,

    Adam

    1. Hi Adam,

      You may have solved your problem by this time -- but if you haven't you should know that that anti-Hu can be sensitive to over-fixation.  For this antibody we fix embryos/larvae for just 2 hours at RT in 4% PFA (or overnight at 4C) -- if you go too long you'll lose the signal.

      - Jim

  2. HI,

    The anti-Hu  16A11 monoclonal   was  originally  designed by our company( http://www.clonegene.com/tumorexpressionreagents/humonoclonals.html) We have found it to be very reliable , feel free to order it from us ! We are happy to offer a discount on bulk orders .

    Dawn

    VP Development

    CLONEGENE