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Assays Tested

Assay

Prep

Worked

Notes

immunohistochemistry

yes

from ZFIN curation

Notes

  • Imported from ZFIN Antibody page Ab1-casr
  • Herberger et al., 2013 : anti-CaSR polyclonal antibody (VA612) that was earlier developed against Mozambique tilapia (Oreochromis mossambicus) CaSR (Loretz et al., 2004). The polypeptide epitope for the VA612 antiserum (tilapia CaSR amino acid residues 46–60; SKDQDLAARPESTQC) differed from the corresponding 15-amino acid sequence in zebrafish (residues 50–64; SKDQDLAARPESTEC) by only a single amino acid residue (Gln59 in tilapia CaSR corresponding in position to Glu63 in zebrafish CaSR). (Herberger, A.L., and Loretz, C.A.)
  • Citations for Ab1-casr at ZFIN