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Antibody Name:

Ab1-bmb from the ZFIN antibody database.

Other names, clone ids, catalog ids etc.

anti-Bmb , bmb

Antibody Registry ID.

Does it work on zebrafish?

yes

Host organism

Rat

Immunogen organism

Zebrafish

Antibody isotype

Antibody type

Anatomical structures recognized
(use terms from the ZFIN Anatomical Ontology)

blastomere condensed chromosome; blastomere karyomere; blastomere nuclear membrane; cell pronucleus

Recognized target molecules (gene names, domains, epitopes ...)

bmb  

Suppliers

Mullins Laboratory

Assays Tested

Assay

Prep

Worked

Notes

immunohistochemistry

yes

from ZFIN curation

Notes

  • Imported from ZFIN Antibody page Ab1-bmb
  • The Bmb ORF corresponding to amino acid residues 43 to 353 was amplified from ovary cDNA using the primer set: 50-CGGAATTCTCCTCCATG GAGAGGC-30 and 50-GCTCGAGTTATCCCTGGATCATGTGC-30. The PCR product was directionally cloned into pET23c (Novagen) using EcoRI and XhoI restriction sites. Recombinant protein was expressed in BL-21 bacteria and purified using standard methods. Purified protein was injected into rats (Covance, PA) using a standard protocol schedule. (Abrams, E.W., Zhang, H., Marlow, F.L., Kapp, L., Lu, S., and Mullins, M.C.)
  • Citations for Ab1-bmb at ZFIN