Child pages
  • Ab1-bmb
Skip to end of metadata
Go to start of metadata

The license could not be verified: There is no license certificate installed for CustomWare Linking Plugin for Confluence. Generate a Free license now.

Antibody Name:

Ab1-bmb from the ZFIN antibody database.

Other names, clone ids, catalog ids etc.

anti-Bmb , bmb

Does it work on zebrafish?

yes

Host organism

Rat

Immunogen organism

Zebrafish

Antibody isotype

Antibody type

Anatomical structures recognized
(use terms from the ZFIN Anatomical Ontology)

blastomere condensed chromosome; blastomere karyomere; blastomere nuclear membrane; cell pronucleus

Recognized target molecules (gene names, domains, epitopes ...)

bmb  

Suppliers

Mullins Laboratory

Assays Tested

Assay

Prep

Worked

Notes

immunohistochemistry

yes

from ZFIN curation

Notes

  • Imported from ZFIN Antibody page Ab1-bmb
  • The Bmb ORF corresponding to amino acid residues 43 to 353 was amplified from ovary cDNA using the primer set: 50-CGGAATTCTCCTCCATG GAGAGGC-30 and 50-GCTCGAGTTATCCCTGGATCATGTGC-30. The PCR product was directionally cloned into pET23c (Novagen) using EcoRI and XhoI restriction sites. Recombinant protein was expressed in BL-21 bacteria and purified using standard methods. Purified protein was injected into rats (Covance, PA) using a standard protocol schedule. (Abrams, E.W., Zhang, H., Marlow, F.L., Kapp, L., Lu, S., and Mullins, M.C.)
  • Citations for Ab1-bmb at ZFIN