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Antibody Name:

Ab1-JAS96 from the ZFIN antibody database.

Other names, clone ids, catalog ids etc.

JAS96

Does it work on zebrafish?

yes

Host organism

Rat

Immunogen organism

Antibody isotype

Antibody type

Anatomical structures recognized
(use terms from the ZFIN Anatomical Ontology)

actinotrichium

Recognized target molecules (gene names, domains, epitopes ...)

Suppliers

Assays Tested

Assay

Prep

Worked

Notes

immunohistochemistry

yes

from ZFIN curation

Notes

  • Imported from ZFIN Antibody page Ab1-JAS96
  • Dogfish (Scyliorhinus canicula) ceratotrichia were used for raising antisera in rats. 0.05 mg of ceratotrichia in 1 ml of PBS 0.1 M at pH 7.3 containing Freund coadyuvant was injected subcutaneously. After a second dose,the third dose was injected intraperitoneally together with incomplete Freund coadyuvant. After a week animals were bled. Immunocytochemistry on tissue sections showed that the antiserum (1 :1000 dilution) selectively bound to actinotrichia. Detects ~22KDa band on Western blots.[Santamaria, et al. 1996,Int J Dev Biol. 1996;Suppl 1:175S-176S]. (ZFIN Staff)
  • Citations for Ab1-JAS96 at ZFIN