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Antibody Name:

Ab1-5D4 from the ZFIN antibody database.

Other names, clone ids, catalog ids etc.

1/20/5-D-4 , anti-keratin sulfate 5D4 epitope , 5D4

Does it work on zebrafish?

yes

Host organism

Mouse

Immunogen organism

Human

Antibody isotype

IgG1 , k

Antibody type

monoclonal

Anatomical structures recognized
(use terms from the ZFIN Anatomical Ontology)

branchiostegal ray; ceratohyal cartilage; cleithrum; hemal arch; hyosymplectic cartilage; Meckel's cartilage; neural arch; notochord; opercular flap; pharyngeal arch 2 skeleton; pharyngeal arch 5 skeleton; quadrate-anguloarticular joint; somite border; vertebra

Recognized target molecules (gene names, domains, epitopes ...)

Suppliers

Assays Tested

Assay

Prep

Worked

Notes

immunohistochemistry

yes

from ZFIN curation

Notes

  • Imported from ZFIN Antibody page Ab1-5D4
  • Monoclonal antibodies were raised against proteoglycan core protein isolated after chondroitinase ABC digestion of human articular cartilage proteoglycan monomer. 5D4 specifically\nrecognizes an antigenic determinant in polysaccharide structure of both corneal and skeletal keratan sulfate. PMID: 6223038 (ZFIN Staff)
  • Citations for Ab1-5D4 at ZFIN