Child pages
  • Ab-A4.1025
Skip to end of metadata
Go to start of metadata

Error rendering macro 'table-plus' : Notify your Confluence administrator that "Bob Swift Atlassian Add-ons - Advanced Tables" requires a valid license. Reason: VERSION_MISMATCH

Assays Tested

Assay

Prep

Worked

Notes

immunohistochemistry

yes

from ZFIN curation

Notes

  • Imported from ZFIN Antibody page Ab-A4.1025
  • Labels both fast and slow sarcomeric MyHCs in an evolutionarily-conserved epitope in the myosin head domain near to the ATP binding site, as shown by Dan-Goor et al. J Muscle Res Cell Motil. 1990 Jun;11(3):216-26.. PMID: 1698201 (ZFIN Staff)
  • Citations for Ab-A4.1025 at ZFIN
  1. The A4.1025 epitope is located near the ATP binding site of the MyHC head, as reported in mammals by Dan Goor et al 1990.  We have found this epitope to be highly conserved in sarcomeric MyHCs from all genes tested in a wide variety of vertebrates.  The epitope is exposed in native myosin, and after 4% PFA and a variety alcoholic fixations.  It is the most reliable general sarcomeric MyHC antibody I know.  It does not detect zebrafish smooth muscle, for example in the early gut, or non-muscle myosins.